Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant

Shin, Dong Ho; Choi, Myoung-Goo; Chen, Kang; Park, Chul‐Soo; Choi, Sang‐Bong; Park, Youn‐Il

doi:10.1007/s13258-015-0373-3

Cited by 20 publications

(9 citation statements)

References 38 publications

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“…The different gene expression and enzyme activity responses might be related to the different cultivar properties, as ‘Golden Delicious’ is a non-red cultivar and ‘Fuji’ is a red cultivar. UFGT catalyzes the glycosylation of both cyanidin and quercetin in apple peel [27,40], while DFR is only involved in the synthesis of cyanidin [30,31,32]. Therefore, under relatively high sunlight conditions, the lower concentrations of quercetin-3-glycoside in DPI-treated ‘Fuji’ peels suggest that DPI may inhibit the synthesis of anthocyanin mainly by affecting the activity of UFGT (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies demonstrated that chalcone isomerase (CHI) and flavanone 3-hydroxylase (F3H) were not key enzymes controlling the synthesis of anthocyanins and flavonols in apple [29]. Hydroflavonol 4-reductase (DFR) is a key enzyme in the biosynthesis of anthocyanins and has been extensively studied in many plants [30,31,32]. In apple peels, CHS , F3H , DFR , ANS, and UFGT achieved higher expression levels in UV-B and low-temperature conditions [33].…”

Section: Introductionmentioning

confidence: 99%

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Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

Chen

Zhang

et al. 2019

IJMS

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Sunlight radiation is a main environmental factor which affects anthocyanin synthesis. To clarify the regulatory mechanism of sunlight on the synthesis of anthocyanin in apple peel, bagged apples were exposed to diverse intensities of sunlight through different shading treatments. Under an increased solar ultraviolet-B (UV-B) light intensity, the concentration of anthocyanin in apple peels was consistent with the Michaelis–Menten equation. Under lower sunlight intensities, diphenyleneiodonium chloride (DPI, an inhibitor of plasma membrane NAD(P)H oxidase) treatment increased both the concentration of cyanidin-3-glycoside and the activity of dihydroflavonol 4-reductase (DFR). However, under higher sunlight intensities, DPI treatment decreased the concentrations of cyanidin-3-glycoside and quercetin-3-glycoside, as well as the activities of DFR and UDP-glycose: flavonoid 3-O-glycosyltransferase (UFGT). These results indicate that, under low sunlight intensity, anthocyanin synthesis in apple peel was limited by the supply of the substrate cyanidin, which was regulated by the DFR activity. Nevertheless, after exposure to high sunlight intensity, the anthocyanin produced in the apple peel was dependent on UFGT activity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

Chen

Zhang

et al. 2019

IJMS

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“…In particular, the role of the phytochrome-mediated signal transduction pathway, associated with temperature and light perception, has been abundantly explored [ 34 , 35 , 36 , 37 ]. In this sense, various phytochrome signalling components in A. thaliana , such as the PHYTOCROME INTERACTING FACTORS (PIFs) and PROTEIN LONG HYPOCOTYL 5 (HY5), have been shown to act as regulators of anthocyanin biosynthesis [ 38 , 39 , 40 , 41 ]. In other plants, this relationship between light perception and anthocyanin accumulation has also been addressed [ 42 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

The Regulation of Floral Colour Change in Pleroma raddianum (DC.) Gardner

et al. 2020

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Floral colour change is a widespread phenomenon in angiosperms, but poorly understood from the genetic and chemical point of view. This article investigates this phenomenon in Pleroma raddianum, a Brazilian endemic species whose flowers change from white to purple. To this end, flavonoid compounds and their biosynthetic gene expression were profiled. By using accurate techniques (Ultra Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UPLC-HRMS)), thirty phenolic compounds were quantified. Five key genes of the flavonoid biosynthetic pathway were partially cloned, sequenced, and the mRNA levels were analysed (RT-qPCR) during flower development. Primary metabolism was also investigated by gas chromatography coupled to mass spectrometry (GC-EIMS), where carbohydrates and organic acids were identified. Collectively, the obtained results suggest that the flower colour change in P. raddianum is determined by petunidin and malvidin whose accumulation coincides with the transcriptional upregulation of early and late biosynthetic genes of the flavonoid pathway, mainly CHS and ANS, respectively. An alteration in sugars, organic acids and phenolic co-pigments is observed together with the colour change. Additionally, an increment in the content of Fe3+ ions in the petals, from the pink to purple stage, seemed to influence the saturation of the colour.

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“…The transgenic flax plants overexpressing genes encoding CHS, CHI, and DFR have been crossed with transgenic flax line(s) that overexpressed the glucosyltransferase (GT) gene to improve protective ability against pathogen infection (Kostyn et al 2012;Mierziak et al 2014). The genes encoding DFR and ANR proteins were chosen at the point of redirection based upon their key regulatory roles in flavan-3-ol/anthocyanin biosynthetic pathway (Singh et al 2009a, b, Kumar et al 2013Kumar and Yadav 2013a, b;Shin et al 2016;Lim et al 2016). The overexpression of CsDFR and CsANR has been documented with improved flavan-3-ol content in transgenic tobacco plants (Kumar et al 2013;Kumar and Yadav 2013a, b).…”

Section: Pyramided Transgenic Tobacco Plants Showed Better Seed Germimentioning

confidence: 99%

“…The overexpression of DFR genes (DFR1 and DFR2) from Populus trichocarpa in tobacco and P. tomentosa has resulted in the accumulation of anthocyanins and monomeric and polymeric flavan-3-ol metabolites (Huang et al 2012). Similarly, overexpression of wheat DFR gene increases anthocyanin accumulation in an Arabidopsis dfr mutant (Shin et al 2016). The ANR from Malus domestica has also been investigated for flavan-3-ol content in transgenic tobacco (Han et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Pyramiding of tea Dihydroflavonol reductase and Anthocyanidin reductase increases flavan-3-ols and improves protective ability under stress conditions in tobacco

Kumar

Yadav

2017

3 Biotech

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Tea (Camellia sinensis) is one of the richest sources of flavan-3-ols, an important class of flavonoids. The expression level of gene-encoded key regulatory enzymes of flavan-3-ol/anthocyanin biosynthetic pathway, dihydroflavonol 4-reductase (DFR) and anthocyanidin reductase (ANR), has been highly correlated with the flavan-3-ol contents and antioxidant activity in tea plant. In the present study, pyramiding of CsDFR and CsANR in tobacco was achieved. However, single transgenic tobacco overexpressing either CsDFR or CsANR was documented earlier. In continuation, pyramided transgenic lines were evaluated for the possible, either same or beyond, effect on flavan-3-ol accumulation and protective ability against biotic and abiotic stresses. The pyramided transgenic lines showed early flowering and improved seed yield. The transcript levels of flavan-3-ol/anthocyanin biosynthetic pathway and related genes in pyramided transgenic lines were upregulated as compared to control tobacco plants. The accumulations of flavan-3-ols were also found to be higher in pyramided transgenic lines than control tobacco plants. In contrast, anthocyanin content was observed to be decreased in pyramided transgenic lines, while DPPH activity was higher in pyramided transgenic lines. In pyramided transgenic lines, strong protective ability against feeding by Spodoptera litura was documented. The seeds of pyramided transgenic lines were also found to have better germination rate under aluminum toxicity as compared to control tobacco plants. Interestingly, the synergistic effect of these two selected genes are not beyond from transgenic lines expressing either CsDFR and CsANR alone as published earlier in terms of flavan-3-ols accumulation. However, the unique flower color and better seed germination rate are some interestingly comparable differences that were reported in pyramided lines in relation to individual transgenic plants. In conclusion, the present results reveal an interesting dynamic between CsDFR and CsANR in modulating flavan-3-ol/anthocyanin levels and functional analysis of stacked CsDFR and CsANR transgenic tobacco lines.

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Overexpressing the wheat dihydroflavonol 4-reductase gene TaDFR increases anthocyanin accumulation in an Arabidopsis dfr mutant

Cited by 20 publications

References 38 publications

Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

The Regulation of Floral Colour Change in Pleroma raddianum (DC.) Gardner

Pyramiding of tea Dihydroflavonol reductase and Anthocyanidin reductase increases flavan-3-ols and improves protective ability under stress conditions in tobacco

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