Overexpression analysis of plant transcription factors

Zhang, James Z.

doi:10.1016/s1369-5266(03)00081-5

Cited by 176 publications

(113 citation statements)

References 94 publications

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“…S5) or at 28°C, a stressful condition for Arabidopsis. This suggests that these RVE genes are primarily clock outputs and that the disruption in rhythmic gene expression seen in RVE1-OX is likely due to neomorphic effects (31). The similar in vitro binding affinities of RVE1 and CCA1 to the EE (Fig.…”

Section: Rve1 Increases Auxin Levelsmentioning

confidence: 83%

REVEILLE1, a Myb-like transcription factor, integrates the circadian clock and auxin pathways

Rawat

Schwartz

Jones

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

241

203

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The circadian clock modulates expression of a large fraction of the Arabidopsis genome and affects many aspects of plant growth and development. We have discovered one way in which the circadian system regulates hormone signaling, identifying a node that links the clock and auxin networks. Auxin plays key roles in development and responses to environmental cues, in part through regulation of plant growth. We have characterized REVEILLE1 (RVE1), a Myb-like, clockregulated transcription factor that is homologous to the central clock genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELON-GATED HYPOCOTYL (LHY). Despite this homology, inactivation of RVE1 does not affect circadian rhythmicity but instead causes a growth phenotype, indicating this factor is a clock output affecting plant development. CCA1 regulates growth via the bHLH transcription factors PHYTOCHROME INTERACTING FACTOR4 (PIF4) and PIF5, but RVE1 acts independently of these genes. RVE1 instead controls auxin levels, promoting free auxin production during the day but having no effect during the night. RVE1 positively regulates the expression of the auxin biosynthetic gene YUCCA8 (YUC8), providing a mechanism for its growth-promoting effects. RVE1 is therefore a node that connects two important signaling networks that coordinate plant growth with rhythmic changes in the environment.growth control ͉ hypocotyl ͉ yucca C ircadian rhythms are approximately 24-h rhythms in physiology or behavior that are generated by an endogenous clock. Circadian rhythms persist in constant environmental conditions; however they can be entrained or set by environmental cues like light/dark or temperature cycles (1). In plants, these rhythms regulate myriad processes including leaf and cotyledon movement, growth, photosynthesis, and timing of the transition to flowering (2). A functional circadian clock provides an adaptive advantage, perhaps by predicting fluctuations in the external environment (3, 4). In all model systems studied, these self-sustained rhythms are generated by a cell-autonomous central oscillator which regulates the expression of many genes involved in metabolic and physiological functions (1). Microarray studies show that nearly one third of Arabidopsis genes are circadian regulated, with peak expression at different times of day (5, 6).In plants, the central oscillator is composed of interlocking feedback loops with both positive and negative transcriptional regulators (1). The central loop is thought to consist of three genes, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), LATE ELON-GATED HYPOCOTYL (LHY), and TIMING OF CAB EXPRES-SION (TOC1). CCA1 and LHY are morning-phased transcription factors with a single Myb-like domain containing a distinctive SHAQKYF motif (7,8). They bind a motif, termed the evening element (EE; AAAATATCT), in the TOC1 promoter to negatively regulate TOC1 expression. TOC1, an evening-phased gene, in turn positively regulates the expression of CCA1 and LHY through an unknown mechanism, thus forming the core clock negative feedback loop (7...

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Section: Rve1 Increases Auxin Levelsmentioning

confidence: 83%

REVEILLE1, a Myb-like transcription factor, integrates the circadian clock and auxin pathways

Rawat

Schwartz

Jones

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

241

203

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“…It has been suggested that disease resistance is associated with fitness costs and that plants have evolved inducible defense mechanism because it is too costly to have defense responses switch on all the time [5,75]. For instance, overexpression of AtWRKY6, AtWRKY18, AtWRKY53 or AtWRKY70 always resulted in small stunted transgenic plants [50,54,76].…”

Section: Discussionmentioning

confidence: 99%

OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1

et al. 2005

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WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways. It has been shown that one Arabidopsis WRKY protein, AtWRKY29/22, is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens. However, little is known about the biological roles of WRKY proteins in rice. In this study, we investigated the expression patterns of rice AtWRKY29/22 homolog, OsWRKY03, under different conditions, and also its possible role involved in plant defense. Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments. Further analysis revealed that the expression of OsWRKY03 was light dependent. Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay. Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein. OsNPR1 as well as several other pathogenesis-related genes, such as OsPR1b, phenylalanine ammonia-lyase (ZB8) and peroxidase (POX22.3), were induced in OsWRKY03-overexpressing transgenic plants. These results indicated that OsWRKY03 is located upstream of OsNPR1 as a transcriptional activator in salicylic acid (SA)-dependent or jasmonic acid (JA)-dependent defense signaling cascades.

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“…Arabidopsis.org/links/insertion.jsp). For transcription factor families, however, such mutants seldom exhibit altered phenotypes, most probably because of partly overlapping functional redundancy among individual members [44]. We have identified more than 40 WRKY knockout lines but rarely observe phenotypic alterations under standard growth conditions (B Ü lker, N Kamphaus, A Zhou, IE Somssich, unpublished).…”

Section: Defining Wrky Functions In Vivomentioning

confidence: 96%

“…Nevertheless, these mutants are a valuable source for the generation of multiple WRKY knockout lines and for extensive phenotypic profiling under defined stress and altered environmental conditions. Ectopic overexpression can also provide information to help define gene function [44]. How useful this approach will be for WRKY factors remains unclear.…”

Section: Defining Wrky Functions In Vivomentioning

confidence: 99%

WRKY transcription factors: from DNA binding towards biological function

Ülker

Somssich

2004

Current Opinion in Plant Biology

861

655

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WRKY proteins comprise a large family of transcription factors. Despite their dramatic diversification in plants, WRKY genes seem to have originated in early eukaryotes. The cognate DNA-binding site of WRKY factors is well defined, but determining the roles of individual family members in regulating specific transcriptional programs during development or in response to environmental signals remains daunting. This review summarises the recent advances made in starting to unravel the various functions controlled by WRKY proteins.Addresses

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Overexpression analysis of plant transcription factors

Cited by 176 publications

References 94 publications

REVEILLE1, a Myb-like transcription factor, integrates the circadian clock and auxin pathways

REVEILLE1, a Myb-like transcription factor, integrates the circadian clock and auxin pathways

OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1

WRKY transcription factors: from DNA binding towards biological function

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