Overexpression and Inactivation of UGT73B2 Modulate Tolerance to Oxidative Stress in Arabidopsis

Kim, In A; Heo, Jung-Ok; Chang, Kwang Suk; Lee, Shin Ae; Lee, Mi-Hyun; Lim, Chae Eun; Lim, Jun

doi:10.1007/s12374-010-9110-2

Cited by 13 publications

(9 citation statements)

References 29 publications

(30 reference statements)

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“…In particular, glycosylation catalyzed by a superfamily of GTs plays significant roles in modulating the solubility, stability, bioavailability and bioactivity of various small molecules [18], 19,41. Recent studies have demonstrated that GTs play important roles in modulating plant tolerance to abiotic stresses [31], [32]. In this study, for the first time, we demonstrated that ectopic expression of the Arabidopsis GT gene UGT85A5 enhanced the adaptation of the transgenic tobacco plants under salt stress.…”

Section: Discussionsupporting

confidence: 53%

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Ectopic Expression of Arabidopsis Glycosyltransferase UGT85A5 Enhances Salt Stress Tolerance in Tobacco

et al. 2013

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Abiotic stresses greatly influence plant growth and productivity. While glycosyltransferases are widely distributed in plant kingdom, their biological roles in response to abiotic stresses are largely unknown. In this study, a novel Arabidopsis glycosyltransferase gene UGT85A5 was identified as significantly induced by salt stress. Ectopic expression of UGT85A5 in tobacco enhanced the salt stress tolerance in the transgenic plants. There were higher seed germination rates, better plant growth and less chlorophyll loss in transgenic lines compared to wild type plants under salt stress. This enhanced tolerance of salt stress was correlated with increased accumulations of proline and soluble sugars, but with decreases in malondialdehyde accumulation and Na+/K+ ratio in UGT85A5-expressing tobacco. Furthermore, during salt stress, expression of several carbohydrate metabolism-related genes including those for sucrose synthase, sucrose-phosphate synthase, hexose transporter and a group2 LEA protein were obviously upregulated in UGT85A5-expressing transgenic plants compared with wild type controls. Thus, these findings suggest a specific protective role of this glycosyltransferase against salt stress and provide a genetic engineering strategy to improve salt tolerance of crops.

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Section: Discussionsupporting

confidence: 53%

“…DIN2 , P450 , RD20 , ORG1 and PCC1 ) leading to increased tolerance to salinity and drought stress in Arabidopsis thaliana [31]. UGT73B2 loss-of-function endowed the mutant with oxidative stress tolerance [32]. Family 1 are the largest GT family that has various potential substrates from plant secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

Ectopic Expression of Arabidopsis Glycosyltransferase UGT85A5 Enhances Salt Stress Tolerance in Tobacco

et al. 2013

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“…Kim et al . () reported that loss of function of UGT73B2 alone or in conjunction with its closest homologs, UGT73B1 and UGT73B3 , confers greater tolerance of Arabidopsis roots to oxidative stress induced by paraquat. These authors suggested that these UGT genes might play a physiological role in the glycosylation of flavonoids in roots.…”

Section: Discussionmentioning

confidence: 99%

“…; Kim et al . ). However, except for UGT73C6 , which encodes a flavonol‐3‐ O ‐rhamnoside‐7‐ O ‐glucosyltransferase (Jones et al .…”

Section: Introductionmentioning

confidence: 97%

The secondary metabolism glycosyltransferases UGT73B3 and UGT73B5 are components of redox status in resistance of Arabidopsis to Pseudomonas syringae pv. tomato

Simon

Langlois‐Meurinne

Didierlaurent

et al. 2013

Plant Cell & Environment

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Secondary metabolism plant glycosyltransferases (UGTs) ensure conjugation of sugar moieties to secondary metabolites (SMs) and glycosylation contributes to the great diversity, reactivity and regulation of SMs. UGT73B3 and UGT73B5, two UGTs of Arabidopsis thaliana (Arabidopsis), are involved in the hypersensitive response (HR) to the avirulent bacteria Pseudomonas syringae pv. tomato (Pst-AvrRpm1), but their function in planta is unknown. Here, we report that ugt73b3, ugt73b5 and ugt73b3 ugt73b5 T-DNA insertion mutants exhibited an accumulation of reactive oxygen species (ROS), an enhanced cell death during the HR to Pst-AvrRpm1, whereas glutathione levels increased in the single mutants. In silico analyses indicate that UGT73B3 and UGT73B5 belong to the early salicylic acid (SA)-induced genes whose pathogen-induced expression is co-regulated with genes related to cellular redox homeostasis and general detoxification. Analyses of metabolic alterations in ugt mutants reveal modification of SA and scopoletin contents which correlate with redox perturbation, and indicate quantitative modifications in the pattern of tryptophan-derived SM accumulation after Pst-AvrRpm1 inoculation. Our data suggest that UGT73B3 and UGT73B5 participate in regulation of redox status and general detoxification of ROSreactive SMs during the HR to Pst-AvrRpm1, and that decreased resistance to Pst-AvrRpm1 in ugt mutants is tightly linked to redox perturbation.

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“…For the RNAi construct, the 449-bp fragment of GAL2 that is also conserved for GAZ, GAL1, and GAL3 was amplified with two different sets of primers as listed in Supplemental Table 3. The two fragments were inserted into the pBluescript II SK(+) vector (pBSK-dGUS) to make a hairpin loop after transcription as described previously (Kim et al, 2010). The fragment in pBSK-dGUS was again PCR amplified with a forward primer containing CACC and a reverse primer as listed in Supplemental Table 3.…”

Section: Plasmid Construction and Plant Transformationmentioning

confidence: 99%

Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue

et al. 2016

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In multicellular organisms, controlling the timing and extent of asymmetric cell divisions (ACDs) is crucial for correct patterning. During post-embryonic root development in Arabidopsis thaliana, ground tissue (GT) maturation involves an additional ACD of the endodermis, which generates two different tissues: the endodermis (inner) and the middle cortex (outer). It has been reported that the abscisic acid (ABA) and gibberellin (GA) pathways are involved in middle cortex (MC) formation. However, the molecular mechanisms underlying the interaction between ABA and GA during GT maturation remain largely unknown. Through transcriptome analyses, we identified a previously uncharacterized C2H2-type zinc finger gene, whose expression is regulated by GA and ABA, thus named GAZ (GA- AND ABA-RESPONSIVE ZINC FINGER). Seedlings ectopically overexpressing GAZ (GAZ-OX) were sensitive to ABA and GA during MC formation, whereas GAZ-SRDX and RNAi seedlings displayed opposite phenotypes. In addition, our results indicated that GAZ was involved in the transcriptional regulation of ABA and GA homeostasis. In agreement with previous studies that ABA and GA coordinate to control the timing of MC formation, we also confirmed the unique interplay between ABA and GA and identified factors and regulatory networks bridging the two hormone pathways during GT maturation of the Arabidopsis root.

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Overexpression and Inactivation of UGT73B2 Modulate Tolerance to Oxidative Stress in Arabidopsis

Cited by 13 publications

References 29 publications

Ectopic Expression of Arabidopsis Glycosyltransferase UGT85A5 Enhances Salt Stress Tolerance in Tobacco

Ectopic Expression of Arabidopsis Glycosyltransferase UGT85A5 Enhances Salt Stress Tolerance in Tobacco

The secondary metabolism glycosyltransferases UGT73B3 and UGT73B5 are components of redox status in resistance of Arabidopsis to Pseudomonas syringae pv. tomato

Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue

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