Overexpression of

Hu, Qing; Chen, Yanhang; Zhao, Yunfeng; Gu, Jun; Ma, Muqing; Li, Hua; Liu, Cong; Wang, Z.‐Y.

doi:10.1071/fp21165

Cited by 9 publications

(4 citation statements)

References 54 publications

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“…In fact, previous phenotypic studies of adult Arabidopsis plants from single mutants in individual SCL genes did not report any visible alterations, with only a quintuple mutant of the four SCL members and the SC35 gene ( scl28 scl30 scl30a scl33 sc35 ) exhibiting clear defects in leaf development and flowering (Yan et al, 2017). In a recent report, heterologous overexpression of the cassava ( Manihot esculenta ) MeSCL30a gene in Arabidopsis plants is described as increasing salt stress sensitivity during seed germination (Hu et al, 2021). However, MeSCL30a also shares high identity with the Arabidopsis SCL33 protein and was expressed at extremely high levels in Arabidopsis plants.…”

Section: Discussionmentioning

confidence: 99%

The SCL30a SR protein regulates ABA‐dependent seed traits and germination under stress

Laloum

Carvalho

Martín

et al. 2023

Plant Cell & Environment

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SR proteins are conserved RNA‐binding proteins best known as splicing regulators that have also been implicated in other steps of gene expression. Despite mounting evidence for a role in plant development and stress responses, the molecular pathways underlying SR protein regulation of these processes remain poorly understood. Here we show that the plant‐specific SCL30a SR protein negatively regulates ABA signaling to control seed traits and stress responses during germination in Arabidopsis. Transcriptome‐wide analyses revealed that loss of SCL30a function barely affects splicing, but largely induces ABA‐responsive gene expression and genes repressed during germination. Accordingly, scl30a mutant seeds display delayed germination and hypersensitivity to ABA and high salinity, while transgenic plants overexpressing SCL30a exhibit reduced ABA and salt stress sensitivity. An ABA biosynthesis inhibitor rescues the enhanced mutant seed stress sensitivity, and epistatic analyses confirm that this hypersensitivity requires a functional ABA pathway. Finally, seed ABA levels are unchanged by altered SCL30a expression, indicating that the gene promotes seed germination under stress by reducing sensitivity to the phytohormone. Our results reveal a new player in ABA‐mediated control of early development and stress response.

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Section: Discussionmentioning

confidence: 99%

The SCL30a SR protein regulates ABA‐dependent seed traits and germination under stress

Laloum

Carvalho

Martín

et al. 2023

Plant Cell & Environment

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“…It was known that the modulation of SR gene isoforms by salt might destabilize the spliceosome complex to accurately recognize the splice sites ( Albaqami et al, 2019 ; Laloum et al, 2021 ). Our previous studies showed that salt stress promotes the alternative 5′ and 3′ splice-site selection and IR events which led to introduce premature termination (nonsense) codons (PTCs) in the pre-mRNA of SR proteins ( Ding et al, 2014 ; Gu et al, 2020 ; Hu et al, 2021 ). Consistent with these studies, several SR proteins were abnormally spliced under high salinity in date palm ( Figure 6B ).…”

Section: Discussionmentioning

confidence: 99%

“…FIERY2/CTD phosphatase-like 1 (FRY2/CPL1) interacted with AtRS40 and AtRS41 proteins, and the loss-of-function mutations showed the hypersensitive phenotype in response to salt and ABA ( Chen et al, 2013 ). Ectopic overexpression of cassava SCL30A and SR34 proteins could improve the salt tolerance in plants ( Gu et al, 2020 ; Hu et al, 2021 ). Therefore, extensive analysis of SR proteins would provide novel insights on their essential role for proper gene expression underlying salt stress in date palms.…”

Section: Discussionmentioning

confidence: 99%

Salt Stress Modulates the Landscape of Transcriptome and Alternative Splicing in Date Palm (Phoenix dactylifera L.)

Zhang²,

Fu³

et al. 2022

Front. Plant Sci.

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Date palm regards as a valuable genomic resource for exploring the tolerance genes due to its ability to survive under the sever condition. Although a large number of differentiated genes were identified in date palm responding to salt stress, the genome-wide study of alternative splicing (AS) landscape under salt stress conditions remains unknown. In the current study, we identified the stress-related genes through transcriptomic analysis to characterize their function under salt. A total of 17,169 genes were differentially expressed under salt stress conditions. Gene expression analysis confirmed that the salt overly sensitive (SOS) pathway genes, such as PdSOS2;1, PdSOS2;2, PdSOS4, PdSOS5, and PdCIPK11 were involved in the regulation of salt response in date palm, which is consistent with the physiological analysis that high salinity affected the Na+/K+ homeostasis and amino acid profile of date palm resulted in the inhibition of plant growth. Interestingly, the pathway of “spliceosome” was enriched in the category of upregulation, indicating their potential role of AS in date palm response to salt stress. Expectedly, many differentially alternative splicing (DAS) events were found under salt stress conditions, and some splicing factors, such as PdRS40, PdRSZ21, PdSR45a, and PdU2Af genes were abnormally spliced under salt, suggesting that AS-related proteins might participated in regulating the salt stress pathway. Moreover, the number of differentially DAS-specific genes was gradually decreased, while the number of differentially expressed gene (DEG)-specific genes was increased with prolonged salt stress treatment, suggesting that AS and gene expression could be distinctively regulated in response to salt stress. Therefore, our study highlighted the pivotal role of AS in the regulation of salt stress and provided novel insights for enhancing the resistance to salt in date palm.

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“…Numerous studies have revealed that the splicing of stress-responsive genes and spliceosome components can be altered by salt stress, which influences the salt tolerance of plants by adjusting the homeostasis of ROS and osmosis. For example, serine/arginine-rich (SR)-like protein, as a component of spliceosome, have been reported to negatively regulate salt stress [ 53 , 54 ]. Among the twelve serine/arginine-rich splicing factors among the selected DEGs in this study, nine genes ( BnaA03g00590D , BnaA06g21030D , BnaA07g06170D , BnaC02g27300D , BnaC07g07660D , BnaC08g16960D , BnaC08g38300D , BnaCnng19170D , Brassica_napus_newGene_614 ) were from the set “down-R. in T.” ( Table S6 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Alkaline Salt Tolerance Genes in Brassica napus L. by Transcriptome Analysis

Tao

Zhu

et al. 2022

Genes

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Soil salt alkalization is one major abiotic factor reducing the productivity of crops, including rapeseed, an indispensable oil crop and vegetable. The mechanism studies of alkali salt tolerance can help breed highly resistant varieties. In the current study, rapeseed (B. napus) line 2205 exhibited more tolerance to alkaline salt than line 1423 did. In line 2205, the lesser plasma membrane damage index, the accumulated osmotic solute, and higher antioxidant enzyme activities contributed to alkaline tolerance. A more integrated mesophyll-cell structure was revealed under alkali salt stress by ultrastructure observation in line 2205, which also implied a lesser injury. Transcriptome analysis showed that more genes responded to alkaline salt in line 2205. The expression of specific-response genes in line 1423 was lower than in line 2205. However, most of the specific-response genes in line 2205 had higher expression, which was mainly enriched in carbohydrate metabolism, photosynthetic processes, ROS regulating, and response to salt stress. It can be seen that the tolerance to alkaline salt is attributed to the high expression of some genes in these pathways. Based on these, twelve cross-differentially expressed genes were proposed as candidates. They provide clues for further analysis of the resistance mechanism of rapeseed.

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Overexpression of

Cited by 9 publications

References 54 publications

The SCL30a SR protein regulates ABA‐dependent seed traits and germination under stress

The SCL30a SR protein regulates ABA‐dependent seed traits and germination under stress

Salt Stress Modulates the Landscape of Transcriptome and Alternative Splicing in Date Palm (Phoenix dactylifera L.)

Identification of Alkaline Salt Tolerance Genes in Brassica napus L. by Transcriptome Analysis

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