Overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from Gossypium hirsutum enhances Arabidopsis resistance to Verticillium wilt

Abstract

Expression of DHS1 in cotton is induced upon infection by Verticillium dahliae , and overexpression of GhDHS1 endows transgenic Arabidopsis plants excellent Verticillium resistance. Verticillium wilt is caused by a soil-borne fungus Verticillium dahliae. Resistance in most cotton cultivars is either scarce or unavailable, making Verticillium wilt a major obstacle in cotton production. Here, we identified a 3-deoxy-7-phosphoheptulonate synthase (DHS, EC 4.1.2.15) gene from Gossypium hirsutum, named GhDHS1. Its 1620 bp open reading frame encodes a putative 59.4 kDa protein. Phylogenetic analysis indicated that GhDHS1 is clustered in a clade with potato and tomato DHSs that can be induced by wounding and elicitors, respectively. Expression analysis demonstrated that GhDHS1 is constitutively expressed in cotton roots and stems, but transcripts are rare or non-existent in the leaves. Subcellular localization showed that GhDHS1 occurs in the plastids. When plants of three cultivars were inoculated with V. dahliae, DHS1 expression was more significantly up-regulated in the roots of resistant G. barbadense cv. Pima90-53 and G. hirsutum cv. Jimian20 than in the susceptible G. hirsutum cv. Han208. This suggested that DHS1 is involved in the cotton resistance to Verticillium wilt. Furthermore, GhDHS1 overexpressing transgenic lines of Arabidopsis were developed via Agrobacterium tumefaciens-mediated transformation. Compared with the untransformed WT (wild type), these transgenic plants showed excellent Verticillium wilt resistance with a significantly lower disease index. The overexpressing transgenic lines also had significantly longer primary roots and greatly increased xylem areas under V. dahliae infection. Overall, our results indicate that GhDHS1 performs a role in the cotton resistance to V. dahliae and would be potential to breeding cottons of Verticillium wilt resistance.

Genome-wide identification of cyclophilin genes in Gossypium hirsutum and functional characterization of a CYP with antifungal activity against Verticillium dahliae

“…The conidial suspension was prepared according to previous description [44] and adjusted to 10 7 spores per milliliter and 10 6 spores per milliliter with distilled water was used to the inoculation of cotton and Arabidopsis , respectively. The plant was infected with V. dahliae using soil drench method [44]. 10 mL of the conidial suspension was directly injected with a needle without piercing into the bottom of each pot.…”

HyPRP1 performs a role in negatively regulating cotton resistance to V. dahliae via the thickening of cell walls and ROS accumulation

“…Localization of fluorescent proteins was monitored 3 days after infiltration using a confocal laser scanning microscope (FluoView FV1000; Olympus). A pCAMBIA derivative (pCamE) carrying a cauliflower mosaic virus 35S–driven GFP was used as the control [ 55 ].…”

Insights into the control of metabolism and biomass accumulation in a staple C4 grass

“…The reported developmental defects in the by1 mutant are interesting given that previous works on DAHPS orthologues have also mainly proposed roles in secondary metabolism. In Arabidopsis and cotton, DAHPS expression is induced upon wounding or pathogen infection ( Keith et al , 1991 ; Yang et al , 2015 ). RNAi silencing of PhDAHP1 in Petunia, one of the two DAHPS isoforms, leads to a reduction of floral volatile benzoid/phenylpropanoids levels ( Langer et al , 2014 ).…”