Overexpression of a 40-kDa Protein in Human Multidrug Resistant Cells

Wang, Ying; Pan, X. Q.; Lheureux, F; Georges, Elias

doi:10.1006/bbrc.1997.6991

Cited by 7 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lung resistance protein (LRP) and breast cancer resistance protein (BCRP) have also been observed in tumor cells , . More recently, the overexpression of a 40 kDa protein (P-40 or Annexin I) was reported in tumor cell lines without MDR1 Pgp , . Since MDR is a major obstacle in chemotherapy of cancer patients, there has been a continuing demand for radiotracers that are able to monitor the tumor MDR transport function in a noninvasive fashion − .…”

Section: Introductionmentioning

confidence: 99%

“…MDA-MB-231 is also an estrogen-independent human breast cancer cell line and is often considered “drug-sensitive” because it has little MDR1, MRP1, and BCRP overexpression , . However, recent studies have clearly demonstrated that the MDA-MB-231 breast tumor cell line has a high level expression of P-40, a 40 kDa protein , . The athymic nude mice bearing KB-3-1 and KB-v-1 tumor xenografts have been successfully used to evaluate cationic 99m Tc and 68 Ga radiotracers − , as well as luminescent probes .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of ⁶⁴Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

et al. 2009

View full text Add to dashboard Cite

In this study, we evaluated the potential of 64 Cu(DO3A-xy-TPEP) (DO3A-xy-TPEP = (2-(diphenylphosphoryl)ethyl)diphenyl(4-((4,7,10-tris(carboxymethyl)-1,4,7,10-tetraazacyclododecan-1-yl)methyl)benzyl)phosphonium) as a PET (positron emission tomography) radiotracer for noninvasive monitoring of multidrug resistance (MDR) transport function in several xenografted tumor models (MDR-negative: U87MG; MDR-positive: MDA-MB-435, MDA-MB-231, KB-3-1 and KB-v-1). It was found that 64 Cu(DO3A-xy-TPEP) has a high initial tumor uptake (5.27 ± 1.2 %ID/g at 5 min p.i.) and show a steady uptake increase between 30 and 120 min p.i. (2.09 ± 0.53 and 3.35 ± 1.27 %ID/g at 30 and 120 min p.i., respectively) in the MDR-negative U87MG glioma tumors. 64 Cu(DO3A-xy-TPEP) has a greater uptake difference between U87MG glioma and MDR-positive tumors (MDA-MB-231: 1.57 ± 0.04, 1.00 ± 0.17, and 0.93 ± 0.15; MDA-MB-435: 1.15 ± 0.19, 1.12 ± 0.20, and 0.81 ± 0.11; KB-3-1: 1.45 ± 0.31, 1.43 ± 0.16, and 1.08 ± 0.19; and KB-v-1: 1.63 ± 0.47, 1.81 ± 0.31, and 1.14 ± 0.22 %ID/g at 30, 60 and 120 min p.i., respectively) than 99m Tc-Sestamibi. Regardless of the source of MDR, the overall net effect is the rapid efflux of 64 Cu(DO3A-xy-TPEP) from tumor cells, which leads to a significant reduction of its tumor uptake. It was concluded that 64 Cu(DO3A-xy-TPEP) is more efficient than 99m Tc-Sestamibi as the substrate for MDR P-glycoproteins (MDR Pgps) and multidrug resistance-associated proteins (MRPs), and might be a more efficient radiotracer for noninvasive monitoring of the tumor MDR transport function. 64 Cu(DO3A-xy-TPEP) and 99m Tc-Sestamibi share almost identical subcellular distribution patterns in U87MG glioma tumors. Thus, it is reasonable to believe that 64 Cu(DO3A-xy-TPEP), like 99m Tc-Sestamibi, is able to localize in mitochondria due to the increased plasma and mitochondrial transmembrane potentials in tumor cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of ⁶⁴Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

et al. 2009

View full text Add to dashboard Cite

show abstract

Annexin-I expression modulates drug resistance in tumor cells

Wang

Serfass²,

Roy³

et al. 2004

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

The role of annexin A1 in expression of matrix metalloproteinase-9 and invasion of breast cancer cells

Kang

Jang

2012

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Overexpression of a 40-kDa Protein in Human Multidrug Resistant Cells

Cited by 7 publications

References 34 publications

Evaluation of ⁶⁴Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

Evaluation of ⁶⁴Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

Annexin-I expression modulates drug resistance in tumor cells

The role of annexin A1 in expression of matrix metalloproteinase-9 and invasion of breast cancer cells

Contact Info

Product

Resources

About

Overexpression of a 40-kDa Protein in Human Multidrug Resistant Cells

Cited by 7 publications

References 34 publications

Evaluation of 64Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

Evaluation of 64Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

Annexin-I expression modulates drug resistance in tumor cells

The role of annexin A1 in expression of matrix metalloproteinase-9 and invasion of breast cancer cells

Contact Info

Product

Resources

About

Evaluation of ⁶⁴Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance

Evaluation of ⁶⁴Cu(DO3A-xy-TPEP) as a Potential PET Radiotracer for Monitoring Tumor Multidrug Resistance