Overexpression of a Brassica rapa NGATHA Gene in Arabidopsis thaliana Negatively Affects Cell Proliferation During Lateral Organ and Root Growth

Kwon, So Hyun; Lee, Byung Ha; Kim, Eun Yu; Seo, Young Sam; Lee, Sangman; Kim, Woo Taek; Song, Jong Tae; Kim, Jeong Hoe

doi:10.1093/pcp/pcp150

Cited by 21 publications

(25 citation statements)

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“…Ten micrograms of total RNAs were subjected to Northern blot analysis, as described by Kwon et al (2009). To prepare gene-specific DNA probes, genomic DNA regions corresponding to the 3′ half and untranslated region of each AtNGA cDNA were amplified by PCR (for primer sequence information, see Online Resource 1).…”

Section: Northern Blot Analysismentioning

confidence: 99%

“…Although Alvarez et al (2009) showed that bigger petals of the nga1-1 mutant resulted from an increase in cell numbers, it remains to be resolved in more detail what exact roles the AtNGA genes play in regulating the cell proliferation activity of lateral organs. In the meantime, we previously demonstrated that Arabidopsis plants overexpressing a Brassica rapa NGA gene (BrNGA1) displayed a remarkable reduction in cell numbers and thus small, narrow lateral organs, leading to the hypothesis that BrNGA1 may also act as a negative regulator of cell proliferation in B. rapa (Kwon et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis thaliana NGATHA transcription factors negatively regulate cell proliferation of lateral organs

et al. 2015

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The cell proliferation process of aerial lateral organs, such as leaves and flowers, is coordinated by complex genetic networks that, in general, converge on the cell cycle. The Arabidopsis thaliana NGATHA (AtNGA) family comprises four members that belong to the B3-type transcription factor superfamily, and has been suggested to be involved in growth and development of aerial lateral organs, although its role in the cell proliferation and expansion processes remains to be resolved in more detail. In order to clarify the role of AtNGAs in lateral organ growth, we took a systematic approach using both the loss- and gain-of-functional mutants of all four members. Our results showed that overexpressors of AtNGA1 to AtNGA4 developed small, narrow lateral organs, whereas the nga1 nga2 nga3 nga4 quadruple mutant produced large, wide lateral organs. We found that cell numbers of the lateral organs were significantly affected: a decrease in overexpressors and, inversely, an increase in the quadruple mutant. Kinematic analyses on leaf growth revealed that, compared with the wild type, the overexpressors displayed a lower activity of cell proliferation and yet the mutant a higher activity. Changes in expression of cell cycle-regulating genes were well in accordance with the cell proliferation activities, establishing that the AtNGA transcription factors act as bona fide negative regulators of the cell proliferation of aerial lateral organs.

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Section: Northern Blot Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Arabidopsis thaliana NGATHA transcription factors negatively regulate cell proliferation of lateral organs

et al. 2015

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“…Five-day-old wild-type and transgenic Arabidopsis leaves were cleared in a chloral hydrate solution as described previously (Kwon et al 2009). The leaf cell layers were visualized by light-field microscopy (BX51 fluorescence microscope, Olympus, Japan).…”

Section: Microscopymentioning

confidence: 99%

Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants

Kim

Seo

Lee

et al. 2010

Planta

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Transient and long-term shortages of fresh water are major adverse environmental factors that cause dramatic reductions in crop production and distribution globally. In this study, we isolated a full-length CaSRP1 (Capsicum annuum stress-related protein 1) cDNA, which was rapidly induced by dehydration in hot pepper plants. The predicted CaSRP1 protein sequence exhibited significant amino acid identity to putative stress-related proteins and the small rubber particle protein (SRPP) found in rubber trees (Hevea brasiliensis). To study the cellular functions of CaSRP1, transgenic Arabidopsis plants (35S:CaSRP1) that constitutively expressed the CaSRP1 gene were constructed. Overexpression of CaSRP1 resulted in enhanced root and shoot growth and earlier bolting in the transgenic plants relative to wild-type plants. In addition, 35S:CaSRP1 overexpressors exhibited enhanced tolerance to drought stress as compared to the control plants. These results suggest that CaSRP1 plays dual functions as a positive factor for tissue growth and development and for drought-defensive responses. A possible cellular function of SRPP homologs in non-rubber-producing plants in relation to drought stress tolerance is discussed.

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“…Arabidopsis overexpressors of BrNGA1 (BrNGA1ox) are prepared as described previously (Kwon et al 2009). The CKX2ox seeds were kindly provided by Dr. Thomas Schmülling.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Recently, we have analyzed Arabidopsis overexpressors of a Brassica rapa NGATHA gene, BrNGA1, which encodes a B3-type transcription factor (Kwon et al 2009). Our study demonstrated that the BrNGA1 transcription factor may be involved in negative regulation of cell proliferation during lateral organ and root growth.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Brassica rapa NGATHA1 Gene Confers De-Etiolation Phenotype and Cytokinin Resistance on Arabidopsis thaliana

Kwon

Chang

et al. 2011

J. Plant Biol.

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Brassica rapa NGATHA1 (BrNGA1) encodes a B3-type transcription factor. By analyzing Arabidopsis overexpressors of BrNGA1 (BrNGA1ox), we have previously demonstrated that BrNGA1 may be involved in negative regulation of cell proliferation during lateral organ and root growth. In the present study, we have found that BrNGA1ox seedlings grown in the dark display de-etiolation phenotypes, such as short hypocotyls, open and elongated cotyledons, and developing true leaves. BrNGA1ox seedlings as well as adult plants and calli are also resistant specifically to exogenous cytokinins. These data raise the possibility that the de-etiolation phenotypes of BrNGA1ox seedlings may result from an alteration in cytokinin response. We set out to test whether the de-etiolation phenotype is due to cytokinin overproduction or constitutively activated cytokinin response. First, BrNGA1ox was crossed to the CKX2ox plant, an overexpression line of CYTOKIN OXIDASE 2, which is responsible for degradation of active cytokinins. We found, however, no difference in the de-etiolation and shoot growth phenotypes between BrNGA1ox and BrNGA1ox CKX2ox plants. Next, we measured the transcripts level of ARR5 and ARR7, frequently employed as molecular markers for cytokinin signaling and yet found no difference in their transcripts levels of the wild-type and BrNGA1ox seedlings and shoots. These data indicate that biological role of BrNGA1 involved in de-etiolation seems to be associated with neither cytokinin overproduction nor its altered signaling. Possible molecular mechanisms by which BrNGA1 may interfere with cytokinin responses and etiolation are discussed.

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Overexpression of a Brassica rapa NGATHA Gene in Arabidopsis thaliana Negatively Affects Cell Proliferation During Lateral Organ and Root Growth

Cited by 21 publications

References 50 publications

The Arabidopsis thaliana NGATHA transcription factors negatively regulate cell proliferation of lateral organs

The Arabidopsis thaliana NGATHA transcription factors negatively regulate cell proliferation of lateral organs

Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants

Overexpression of Brassica rapa NGATHA1 Gene Confers De-Etiolation Phenotype and Cytokinin Resistance on Arabidopsis thaliana

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