Overexpression of a Cytosolic 6-Phosphogluconate Dehydrogenase Gene Enhances the Resistance of Rice to Nilaparvata lugens

Abstract

The pentose phosphate pathway (PPP) plays an important role in plant growth and development, and plant responses to biotic and abiotic stresses. Yet, whether the PPP regulates plant defenses against herbivorous insects remains unclear. In this study, we cloned a rice cytosolic 6-phosphogluconate dehydrogenase gene, Os6PGDH1, which encodes the key enzyme catalyzing the third step in the reaction involving the oxidative phase of the PPP, and explored its role in rice defenses induced by brown planthopper (BPH) Nilaparvata lugens. Levels of Os6PGDH1 transcripts were detected in all five examined tissues, with the highest in outer leaf sheaths and lowest in inner leaf sheaths. Os6PGDH1 expression was strongly induced by mechanical wounding, infestation of gravid BPH females, and jasmonic acid (JA) treatment. Overexpressing Os6PGDH1 (oe6PGDH) decreased the height of rice plants and the mass of the aboveground part of plants, but slightly increased the length of plant roots. In addition, the overexpression of Os6PGDH1 enhanced levels of BPH-induced JA, jasmonoyl-isoleucine (JA-Ile), and H2O2, but decreased BPH-induced levels of ethylene. Bioassays revealed that gravid BPH females preferred to feed and lay eggs on wild-type (WT) plants over oe6PGDH plants; moreover, the hatching rate of BPH eggs raised on oe6PGDH plants and the fecundity of BPH females fed on these were significantly lower than the eggs and the females raised and fed on WT plants. Taken together, these results indicate that Os6PGDH1 plays a pivotal role not only in rice growth but also in the resistance of rice to BPH by modulating JA, ethylene, and H2O2 pathways.

Transcriptome integrated metabolic modeling of carbon assimilation underlying storage root development in cassava

“…Deletion of the G6PDH gene results in the overproduction of reaction oxygen species (ROS), reductions in root elongation and germination rate of plants under stress, and a decrease in plant productivity 57 , 59 . Overexpression of the G6PDH gene induced drought tolerance in transgenic tobacco 58 and increased the length of roots during early development in rice 60 . Regulation of the G6PDH enzyme in the chloroplast of photosynthetic tissues is governed by the ferredoxin/thioredoxin system under darkness, while high sugar levels in cytosol of non-photosynthetic tissue enhance the transcription of the G6PDH gene 61 .…”

OsG6PGH1 affects various grain quality traits and participates in the salt stress response of rice

“…G6PGH plays an important role in the response of plants to abiotic stress ( Chen et al., 2020 ). Transgenic G6PGH in maize can significantly improve heat tolerance, thereby increasing maize yield under high temperature stress ( Ribeiro et al., 2020 ).…”

Fatty acyl-CoA reductase influences wax biosynthesis in the cotton mealybug, Phenacoccus solenopsis Tinsley

“…The sense and antisense strands of the purified PsFAR fragment were respectively double digested by Sal I + Apa I and Sac I + Bam HI. Two digested strands were inserted into corresponding restriction sites of the plant expression vector pCAMBIA1301 63 one at a time. The resulting RNAi transformation vector pCAMBIA1301-ds PsFAR was validated by sequencing (Tsingke Biotech, Beijing, China).…”