Overexpression of a ItICE1 gene from Isatis tinctoria enhances cold tolerance in rice

Xiang, Dian-Jun; Man, Lili; Yin, Kuide; Qunyan, Song; Wang, Lina; Zhao, Mingzhu; Xu, Zhenbo

doi:10.1007/s11032-013-9894-0

Cited by 21 publications

(5 citation statements)

References 44 publications

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“…For example, in A. thaliana , several lipid catabolism enzymes in rice (in particular, phospholipids A and D) are activated by low temperatures, as manifested by the heightened accumulation of fatty acids ( Wang et al, 2006 ; Usadel et al, 2008 ). Serine phosphorylation or dephosphorylation is involved in cold activation signaling of Arabidopsis ICE1 , and its Ox in Isatis tinctoria confers cold tolerance ( Chinnusamy et al, 2003 ; Xiang et al, 2013 ). Campos et al (2003) have reported that a cold-tolerant genotype of Coffea sp.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Genes, Conserved between japonica and indica Rice Cultivars, that Respond to Low-Temperature Stress at the Vegetative Growth Stage

et al. 2017

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Cold stress is very detrimental to crop production. However, only a few genes in rice have been identified with known functions related to cold tolerance. To meet this agronomic challenge more effectively, researchers must take global approaches to select useful candidate genes and find the major regulatory factors. We used five Gene expression omnibus series data series of Affymetrix array data, produced with cold stress-treated samples from the NCBI Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/), and identified 502 cold-inducible genes common to both japonica and indica rice cultivars. From them, we confirmed that the expression of two randomly chosen genes was increased by cold stress in planta. In addition, overexpression of OsWRKY71 enhanced cold tolerance in ‘Dongjin,’ the tested japonica cultivar. Comparisons between japonica and indica rice, based on calculations of plant survival rates and chlorophyll fluorescence, confirmed that the japonica rice was more cold-tolerant. Gene Ontology enrichment analysis indicate that the ‘L-phenylalanine catabolic process,’ within the Biological Process category, was the most highly overrepresented under cold-stress conditions, implying its significance in that response in rice. MapMan analysis classified ‘Major Metabolic’ processes and ‘Regulatory Gene Modules’ as two other major determinants of the cold-stress response and suggested several key cis-regulatory elements. Based on these results, we proposed a model that includes a pathway for cold stress-responsive signaling. Results from our functional analysis of the main signal transduction and transcription regulation factors identified in that pathway will provide insight into novel regulatory metabolism(s), as well as a foundation by which we can develop crop plants with enhanced cold tolerance.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Genes, Conserved between japonica and indica Rice Cultivars, that Respond to Low-Temperature Stress at the Vegetative Growth Stage

et al. 2017

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“…As master regulators of CBF expression, ICE binds to the promoter of the CBF genes, and positively induces the expression of CBF genes (Chinnusamy et al 2003). Furthermore, overexpression of the ICE1 gene enhanced plant tolerance to cold stress, whereas wild plants were hypersensitive to cold stress (Xiang et al 2013, 2017). The ICE2 gene is highly homologous to ice1 , it has been found to have the ability to trigger the expression of CBF1 (Fursova et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

Cai

Magwanga

et al. 2019

AoB PLANTS

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Low temperature is one of the key environmental stresses that impair plant growth and significantly restricts the productivity and spatial distribution of crop plants. Gossypium thurberi, a wild diploid cotton species, has adapted to a wide range of temperatures and exhibits a better tolerance to chilling stress. Here, we compared phenotypes and physiochemical changes in G. thurberi under cold stress and found this species indeed showed better cold tolerance. Therefore, to understand the molecular mechanisms of the cold tolerance in G. thurberi, we compared transcription changes in leaves of G. thurberi under cold stress by high-throughput transcriptome sequencing. In total, 35 617 unigenes were identified in the whole-genome transcription profile, and 4226 differentially expressed genes (DEGs) were discovered in the leaves upon cold treatment. Gene Ontology (GO) classification analyses showed that the majority of DEGs belonged to categories of signal transduction, transcription factors (TFs) and carbohydrate transport and metabolism. The expression of several cold-responsive genes such as ICE1, CBF4, RAP2-7 and abscisic acid (ABA) biosynthesis genes involved in different signalling pathways were induced after G. thurberi seedlings were exposed to cold stress. Furthermore, cold sensitivity was increased in CBF4 and ICE2 virus-induced gene silencing (VIGS) plants, and high level of malondialdehyde (MDA) showed that the CBF4 and ICE2 silenced plants were under oxidative stress compared to their wild types, which relatively had higher levels of antioxidant enzyme activity, as evident by high levels of proline and superoxide dismutase (SOD) content. In conclusion, our findings reveal a new regulatory network of cold stress response in G. thurberi and broaden our understanding of the cold tolerance mechanism in cotton, which might accelerate functional genomics studies and genetic improvement for cold stress tolerance in cultivated cotton.

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“…The overexpression of ICE1 in plants under normal growth conditions has a small effect on plant growth. Transgenic ICE1expressing rice [14], tomato [15], Arabidopsis [16,17], tobacco [18], and lemon [7] plants show normal growth, with very few instances of mutation or dwarfing, indicating that the introduction of ICE1 has fewer adverse effects on plant growth than the introduction of CBF/DREB1. When CBF/DREB expression increases in a plant, COR expression also increases, which regulates the expression of a series of genes and changes the physiological and biochemical indicators of the plant itself, resulting in the plant having a certain cold tolerance.…”

Section: Discussionmentioning

confidence: 99%

Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

Qu¹,

Liu²,

Jiao³

et al. 2022

Phyton

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To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations. Under low temperature-stress conditions (4°C), the relative conductivity levels decreased by 27.51%-31.44%, the proline concentrations increased by 12.50%-17.50%, the malondialdehyde concentrations decreased by 16.78%-18.37%, and the peroxidase activities increased by 19.60%-22.89% in the T1 lines compared with those of the control. A real-time quantitative PCR analysis showed that the ICE1 gene was ectopically expressed in the roots, stems, and leaves of the T1 lines. ICE1 positively regulates the expression of the CBF genes in response to cold stress. Thus, this study showed the successful transformation of maize with the ICE1 gene, resulting in the generation of a new maize germplasm that had increased tolerance to cold stress.

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Overexpression of a ItICE1 gene from Isatis tinctoria enhances cold tolerance in rice

Cited by 21 publications

References 44 publications

Genome-Wide Identification and Analysis of Genes, Conserved between japonica and indica Rice Cultivars, that Respond to Low-Temperature Stress at the Vegetative Growth Stage

Genome-Wide Identification and Analysis of Genes, Conserved between japonica and indica Rice Cultivars, that Respond to Low-Temperature Stress at the Vegetative Growth Stage

Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

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