Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth

Wang, Yanpeng; Feng, Jiancan; Qin, Qiaoping; Huang, Jianqin

doi:10.1007/s11240-015-0817-0

Cited by 22 publications

(7 citation statements)

References 41 publications

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“…In transgenic cotton overexpressing a potato Sus gene, expansion of young leaves is accelerated with significantly increased fructose concentrations and a slight decline for sucrose ( Xu et al, 2012 ). Similar results are observed in transgenic tobacco overexpressing a loquat VIN ( Wang et al, 2015 ). Existing knowledge on sucrose-metabolizing enzymes during leaf development has mainly been drawn from studies on temperate species ( Huber, 1989 ; Foyer and Paul, 2001 ; Roitsch and González, 2004 ; Ruan, 2014 ).…”

Section: Introductionsupporting

confidence: 82%

Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis

Zhu

Fang

et al. 2018

Front. Plant Sci.

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Sucrose-metabolizing enzymes in plant leaves have hitherto been investigated mainly in temperate plants, and rarely conducted in tandem with gene expression and sugar analysis. Here, we investigated the sugar content, gene expression, and the activity of sucrose-metabolizing enzymes in the leaves of Hevea brasiliensis, a tropical tree widely cultivated for natural rubber. Sucrose, fructose and glucose were the major sugars detected in Hevea leaves at four developmental stages (I to IV), with starch and quebrachitol as minor saccharides. Fructose and glucose contents increased until stage III, but decreased strongly at stage IV (mature leaves). On the other hand, sucrose increased continuously throughout leaf development. Activities of all sucrose-cleaving enzymes decreased markedly at maturation, consistent with transcript decline for most of their encoding genes. Activity of sucrose phosphate synthase (SPS) was low in spite of its high transcript levels at maturation. Hence, the high sucrose content in mature leaves was not due to increased sucrose-synthesizing activity, but more to the decline in sucrose cleavage. Gene expression and activities of sucrose-metabolizing enzymes in Hevea leaves showed striking differences compared with other plants. Unlike in most other species where vacuolar invertase predominates in sucrose cleavage in developing leaves, cytoplasmic invertase and sucrose synthase (cleavage direction) also featured prominently in Hevea. Whereas SPS is normally responsible for sucrose synthesis in plant leaves, sucrose synthase (synthesis direction) was comparable or higher than that of SPS in Hevea leaves. Mature Hevea leaves had an unusually high sucrose:starch ratio of about 11, the highest reported to date in plants.

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Section: Introductionsupporting

confidence: 82%

Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis

Zhu

Fang

et al. 2018

Front. Plant Sci.

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“…This was also the case in the neutral invertase gene family of grapes and some alkaline/neutral invertase genes of cassava [ 11 , 49 ]. The differential expression patterns of INV s in various tissues reveals that these genes play an important role in their respective organs in terms of providing carbohydrates for growth and development [ 49 , 50 ]. In carrots and tomatoes, some cell wall or vacuolar invertase genes showed markedly different organ- and development-stage-specific expression patterns, and both contain a flower-specific acid invertase gene [ 51 – 53 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the Invertase Gene Family in Populus

Chen¹,

Gao²,

et al. 2015

PLoS ONE

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Invertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which are targeted to the cell wall and vacuole; and neutral/alkaline invertases, which function in the cytosol. In this study, 5 cell wall invertase genes (PtCWINV1-5), 3 vacuolar invertase genes (PtVINV1-3) and 16 neutral/alkaline invertase genes (PtNINV1-16) were identified in the Populus genome and found to be distributed on 14 chromosomes. A comprehensive analysis of poplar invertase genes was performed, including structures, chromosome location, phylogeny, evolutionary pattern and expression profiles. Phylogenetic analysis indicated that the two sub-families were both divided into two clades. Segmental duplication is contributed to neutral/alkaline sub-family expansion. Furthermore, the Populus invertase genes displayed differential expression in roots, stems, leaves, leaf buds and in response to salt/cold stress and pathogen infection. In addition, the analysis of enzyme activity and sugar content revealed that invertase genes play key roles in the sucrose metabolism of various tissues and organs in poplar. This work lays the foundation for future functional analysis of the invertase genes in Populus and other woody perennials.

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“…Exogenous sucrose supplies increase invertase activity in sugarcane [14,15]. The overexpression of invertases accelerate sucrose hydrolysis and enhance plant growth in cotton, Arabidopsis, and loquat [16,17]. On the other hand, the downregulation of SAI by foliar chemical treatment or the inhibition of SAI activity increases sucrose content in sugarcane [18,19].…”

mentioning

confidence: 99%

Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane

Anur

Mufithah

Sawitri

et al. 2020

Plants

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Sucrose phosphate synthase (SPS) is a key enzyme in sucrose synthesis, which controls sucrose content in plants. This study was designed to examine the efficacy of the overexpression of SoSPS1 gene on sucrose accumulation and carbon partitioning in transgenic sugarcane. The overexpression of SoSPS1 gene increased SPS activity and sucrose content in transgenic sugarcane leaves. More importantly, the overexpression enhanced soluble acid invertase (SAI) activity concomitant with the increase of glucose and fructose levels in the leaves, whereas sucrose synthase activity exhibited almost no change. In the stalk, a similar correlation was observed, but a higher correlation was noted between SPS activity and sugar content. These results suggest that SPS overexpression has both direct and indirect effects on sugar concentration and SAI activity in sugarcane. In addition, SPS overexpression resulted in a significant increase in plant height and stalk number in some transgenic lines compared to those in non-transgenic control. Taken together, these results strongly suggest that enhancing SPS activity is a useful strategy for improving sugarcane yield.

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Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth

Cited by 22 publications

References 41 publications

Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis

Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis

Genome-Wide Identification of the Invertase Gene Family in Populus

Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane

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