Overexpression of a Monocot Acyl‐CoA‐Binding Protein Confers Broad‐Spectrum Pathogen Protection in a Dicot

Abstract

Plants are continuously infected by various pathogens throughout their lifecycle. Previous studies have reported that the expression of Class III acyl-CoA-binding proteins (ACBPs) such as the Arabidopsis ACBP3 and rice ACBP5 were induced by pathogen infection. Transgenic Arabidopsis AtACBP3-overexpressors (AtACBP3-OEs) displayed enhanced protection against the bacterial biotroph, Pseudomonas syringae, although they became susceptible to the fungal necrotroph Botrytis cinerea. A Class III ACBP from a monocot, rice (Oryza sativa) OsACBP5 was overexpressed in the dicot Arabidopsis. The resultant transgenic Arabidopsis lines conferred resistance not only to the bacterial biotroph P. syringae but to fungal necrotrophs (Rhizoctonia solani, B. cinerea, Alternaria brassicicola) and a hemibiotroph (Colletotrichum siamense). Changes in protein expression in R. solani-infected Arabidopsis OsACBP5-overexpressors (OsACBP5-OEs) were demonstrated using proteomic analysis. Biotic stress-related proteins including cell wallrelated proteins such as FASCILIN-LIKE ARABINOGALACTAN-PROTEIN10, LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEINS, XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN4, and PECTINESTERASE INHIBITOR18; proteins associated with glucosinolate degradation including GDSL-LIKE LIPASE23, EPITHIOSPECIFIER MODIFIER1, MYROSINASE1, MYROSINASE2, and NITRILASE1; as well as a protein involved in jasmonate biosynthesis, ALLENE OXIDE CYCLASE2, were induced in OsACBP5-OEs upon R. solani infection. These results indicated that upregulation of these proteins in OsACBP5-OEs conferred protection against various plant pathogens.

The Resistance of Narrow-Leafed Lupin to Diaporthe toxica Is Based on the Rapid Activation of Defense Response Genes

“…In contrast, during the setup of an arbuscular mycorrhizal symbiosis between Medicago truncatula and Glomus versiforme , the induction of an XTH gene was observed [ 124 ]. XTH genes are involved in plant defense responses to inoculation by pathogenic fungi, as evidenced by interactions between Arabidopsis and the necrotrophic Rhizoctonia solani (the causal agent of root rot disease), between wild jute species and the hemibiotrophic Macrophomina phaseolina (causing stem rot disease), and between sugarcane and a biotrophic Sporisorium scitamineum (responsible for culmicolous disease) [ 125 , 126 , 127 ]. XTH genes were also revealed to be involved in the phloem response to aphid infestation of celery ( Apium graveolens ) and Arabidopsis [ 128 , 129 ].…”

The overexpression of OsACBP5 protects transgenic rice against necrotrophic, hemibiotrophic and biotrophic pathogens

“…A recent study has shown that transgenic Arabidopsis overexpressing OsACBP5 were conferred resistance to necrotrophic ( R. solani, B. cinerea, A. brassicicola ), hemibiotrophic ( C. siamense ) and biotrophic ( P. syringae ) phytopathogens 59 , 60 . Proteomic analysis on the R. solani- infected transgenic Arabidopsis OsACBP-OEs showed upregulation of biotic stress-related proteins including cell wall-related proteins such as FASCILIN-LIKE ARABINOGALACTAN-PROTEIN10, LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEINS, XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN4 and PECTINESTERASE INHIBITOR18; proteins associated with glucosinolate degradation including GDSL-LIKE LIPASE23, EPITHIOSPECIFIER MODIFIER1, MYROSINASE1, MYROSINASE2 and NITRILASE1; as well as a protein involved in jasmonate biosynthesis, ALLENE OXIDE CYCLASE2 59 , 60 . These results from proteomic analysis indicated that the defence responses arising from OsACBP5 overexpression in transgenic Arabidopsis involved cell wall-mediated defence as well as salicylic acid (SA)- and jasmonic acid (JA)-mediated defence pathways 59 , 60 .…”

“…Rice blast is a destructive fungal disease causing a 30% decline in rice production globally 58 . Our recent study demonstrated that overexpression of OsACBP5 in transgenic Arabidopsis conferred resistance to representative necrotrophic ( R. solani, B. cinerea, Alternaria brassicicola ), hemibiotrophic ( Colletotrichum siamense ) and biotrophic ( P. syringae ) phytopathogens through cell wall-mediated defence as well as SA- and JA-mediated defence pathways 59 , 60 . Given the need for crops to be protected against necrotrophic fungal pathogens, the present study follows up on our initial investigations on OsACBP5 31 .…”

Overexpression of HMG-CoA synthase promotes Arabidopsis root growth and adversely affects glucosinolate biosynthesis

“…Preparation of protein extracts from Arabidopsis roots and mass spectrometry analysis of peptides were performed as previously described (Zhu et al , 2016; Panthapulakkal Narayanan et al , 2019). A global false discovery rate (FDR) (<1%) was used as the criterion to accept peptide assignments and protein identifications (Zhu et al , 2016; Panthapulakkal Narayanan et al , 2019). Four biological repeats of 7-day-old Arabidopsis roots treated with F-244 (2 µM) or DMSO (control) for 4 d were used.…”