Overexpression of a multifunctional β-glucosidase gene from thermophilic archaeon Sulfolobus solfataricus in transgenic tobacco could facilitate glucose release and its use as a reporter

Huang, Chia-Hui; Huang, Tzu Ling; Liu, Yu-Chang; Chen, Ting Chieh; Lin, Shih Ming; Shaw, Sunil K.; Chang, Chung‐Liang

doi:10.1007/s11248-020-00212-z

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…There have been numerous reviews summarized in the field [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. Many types of recombinant proteins, including industrial proteins (e.g., cellulase) [ 42 , 43 ], biopolymers (e.g., spidroin) [ 44 ], and pharmaceutical proteins (e.g., EGF) [ 25 ] have been reported to be successfully expressed in plants.…”

Section: Introductionmentioning

confidence: 99%

Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts

et al. 2023

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Background Chloroplast transformation is a robust technology for the expression of recombinant proteins. Various types of pharmaceutical proteins including growth factors have been reported in chloroplasts via chloroplast transformation approach at high expression levels. However, high expression of epidermal growth factor (EGF) in chloroplasts with the technology is still unavailable. Results The present work explored the high-level expression of recombinant EGF, a protein widely applied in many clinical therapies, in tobacco chloroplasts. In this work, homoplastic transgenic plants expressing fusion protein GFP-EGF, which was composed of GFP and EGF via a linker, were generated. The expression of GFP-EGF was confirmed by the combination of green fluorescent observation and Western blotting. The achieved accumulation of the recombinant fusion GFP-EGF was 10.21 ± 0.27% of total soluble proteins (1.57 ± 0.05 g kg− 1 of fresh leaf). The chloroplast-derived GFP-EGF was capable of increasing the cell viability of the NSLC cell line A549 and enhancing the phosphorylation level of the EGF receptor in the A549 cells. Conclusion The expression of recombinant EGF in tobacco chloroplasts via chloroplast transformation method was achieved at considerable accumulation level. The attempt gives a good example for the application of chloroplast transformation technology in recombinant pharmaceutical protein production.

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Section: Introductionmentioning

confidence: 99%

Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts

et al. 2023

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Dietary supplementation of Synechococcus elongatus PCC 7942 expressing a heterologous β-glucosidase on the expression of genes related to digestion, immune system, and antioxidant defenses of the shrimp Litopenaeus vannamei

Medeiros

Azevedo²,

Riet³

et al. 2022

J Appl Phycol

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Label-free biological sample detection and non-contact separation system based on microfluidic chip

Zhang

Yang

et al. 2022

Review of Scientific Instruments

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The detection and separation of biological samples are of great significance for achieving accurate diagnoses and state assessments. Currently, the detection and separation of cells mostly adopt labeling methods, which will undoubtedly affect the original physiological state and functions of cells. Therefore, in this study, a label-free cell detection method based on microfluidic chips is proposed. By measuring the scattering of cells to identify cells and then using optical tweezers to separate the target cells, the whole process without any labeling and physical contact could realize automatic cell identification and separation. Different concentrations of 15 µm polystyrene microspheres and yeast mixed solution are used as samples for detection and separation. The detection accuracy is over 90%, and the separation accuracy is over 73%.

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Overexpression of a multifunctional β-glucosidase gene from thermophilic archaeon Sulfolobus solfataricus in transgenic tobacco could facilitate glucose release and its use as a reporter

Cited by 3 publications

References 47 publications

Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts

Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts

Dietary supplementation of Synechococcus elongatus PCC 7942 expressing a heterologous β-glucosidase on the expression of genes related to digestion, immune system, and antioxidant defenses of the shrimp Litopenaeus vannamei

Label-free biological sample detection and non-contact separation system based on microfluidic chip

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