Overexpression of a novel NAC-type tomato transcription factor, SlNAM1 , enhances the chilling stress tolerance of transgenic tobacco

Li, Xiaodong; Zhuang, Kunyang; Li, Zhong‐Ming; Yang, Dong-Yue; Ma, Nana; Meng, Qingwei

doi:10.1016/j.jplph.2016.06.024

Cited by 55 publications

(36 citation statements)

References 45 publications

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“…Chlorophyll content was measured by ethanol-acetone extraction [30]. Malondialdehyde (MDA) content was measured by two-component spectrophotometry [31]. Free proline content was measured by acidic ninhydrin colorimetric assay [32].…”

Section: Measurement Of Physiological Parameters and Biomassmentioning

confidence: 99%

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Han

Wang

Wan

et al. 2020

IJERPH

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This study investigated the beneficial effect of molybdenum (Mo) application on rape plants (Brassica napus L.) grown in a soil polluted by cadmium (Cd). A pot experiment was conducted to determine how different concentrations of exogenous Mo (0, 50, 100, and 200 mg/kg) affect plant physiology, biomass, photosynthesis, cation uptake, and Cd translocation and enrichment in rape plants under Cd stress (0.5 and 6.0 mg/kg). Under single Cd treatment, plant physiological and biochemical parameters, biomass parameters, leaf chlorophyll fluorescence parameters, and macroelement uptake of rape plants decreased, while their malonaldehyde content, proline content, non-photochemical quenching coefficient, and Cd uptake significantly increased, compared to those of the control group (p-values < 0.05). High-Cd treatment resulted in much larger changes in these parameters than low-Cd treatment. Following Mo application, the accumulation of malondialdehyde and proline decreased in the leaves of Cd-stressed plants; reversely, the contents of soluble protein, soluble sugar, and chlorophyll, and the activities of superoxide dismutase and glutathione peroxidase, all increased compared to those of single Cd treatment (p-values < 0.05). Exogenous Mo application promoted shoot and root growth of Cd-stressed plants in terms of their length, fresh weight, and dry weight. The negative effect of Cd stress on leaf chlorophyll fluorescence was substantially mitigated by applying Mo. Exogenous Mo also improved the uptake of inorganic cations, especially potassium (K+), in Cd-stressed plants. After Mo application, Cd uptake and accumulation were inhibited and Cd tolerance was enhanced, but Cd translocation was less affected in Cd-stressed plants. The mitigation effect of Mo on Cd stress in rape was achieved through the immobilization of soil Cd to reduce plant uptake, and improvement of plant physiological properties to enhance Cd tolerance. In conclusion, exogenous Mo can effectively reduce Cd toxicity to rape and the optimal Mo concentration was 100 mg/kg under the experimental conditions.

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Section: Measurement Of Physiological Parameters and Biomassmentioning

confidence: 99%

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Han

Wang

Wan

et al. 2020

IJERPH

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“…It is a close homologue of Arabidopsis ATAF1 (Arabidopsis thaliana ACTIVATING FACTOR 1, also called Arabidopsis NAC002, ANAC002; 68% identity and 72% similarity at the amino acid level) whose expression is induced during leaf senescence and by various abiotic stresses, such as H 2 O 2 treatment, drought, salinity, prolonged darkness and that mediates multiple functions in the adaptation to abiotic and biotic stresses (Jensen et al, 2007;Lu et al, 2007;Jensen et al, 2008;Wang et al, 2009;Garapati et al, 2015;Li et al, 2016) (PLAZA 3.0; http://bioinformatics. It is a close homologue of Arabidopsis ATAF1 (Arabidopsis thaliana ACTIVATING FACTOR 1, also called Arabidopsis NAC002, ANAC002; 68% identity and 72% similarity at the amino acid level) whose expression is induced during leaf senescence and by various abiotic stresses, such as H 2 O 2 treatment, drought, salinity, prolonged darkness and that mediates multiple functions in the adaptation to abiotic and biotic stresses (Jensen et al, 2007;Lu et al, 2007;Jensen et al, 2008;Wang et al, 2009;Garapati et al, 2015;Li et al, 2016) (PLAZA 3.0; http://bioinformatics.…”

Section: Sltaf1 Expression Is Induced By Abiotic Stressesmentioning

confidence: 99%

Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress

et al. 2019

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Salinity stress limits plant growth and has a major impact on agricultural productivity. Here, we identify NAC transcription factor SlTAF1 as a regulator of salt tolerance in cultivated tomato (Solanum lycopersicum).While overexpression of SlTAF1 improves salinity tolerance compared with wild-type, lowering SlTAF1 expression causes stronger salinity-induced damage. Under salt stress, shoots of SlTAF1 knockdown plants accumulate more toxic Na + ions, while SlTAF1 overexpressors accumulate less ions, in accordance with an altered expression of the Na + transporter genes SlHKT1;1 and SlHKT1;2. Furthermore, stomatal conductance and pore area are increased in SlTAF1 knockdown plants during salinity stress, but decreased in SlTAF1 overexpressors.We identified stress-related transcription factor, abscisic acid metabolism and defence-related genes as potential direct targets of SlTAF1, correlating it with reactive oxygen species scavenging capacity and changes in hormonal response. Salinity-induced changes in tricarboxylic acid cycle intermediates and amino acids are more pronounced in SlTAF1 knockdown than wild-type plants, but less so in SlTAF1 overexpressors. The osmoprotectant proline accumulates more in SlTAF1 overexpressors than knockdown plants.In summary, SlTAF1 controls the tomato's response to salinity stress by combating both osmotic stress and ion toxicity, highlighting this gene as a promising candidate for the future breeding of stress-tolerant crops.

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“…SlNAM1, SlSRN1, SlNAC3&4 belong to the NAM subfamily. SlNAM1, mostly induced by chilling stress (4°C), treatment of polyethylene glycol (PEG), salt (NaCl), ABA and MeJA, confers chilling stress tolerance to both growing tomato plants as well as germinating seeds (Yang et al 2011;Li et al 2016). SlSRN1 transcription factor defends the plant against Botrytis cinerea and Pst DC3000 infection ).…”

Section: Roles Of Nac Transcription Factors In Tomato (Solanum Lycopementioning

confidence: 99%

“…Among plant transcription factors are the families of AP2/EREBP (APETALA2/Ethylene-Responsive Element Binding Protein), bZIP (Basic Leucine Zipper), zinc finger, NAC (NAM, ATAF1&2, CUC2), MYB (myeloblastosis) (Ulm et al 2004;Hirota et al 2007), and WRKY (Rushton et al 2010). Among these, plant-specific NAC transcription factors constitute one of the largest families of transcription factors in plants (Nuruzzaman et al 2013;Li et al 2016) and they are known for their roles in many plant growth and developmental processes as well as abiotic and abiotic stress responses (Nakashima et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

Tweneboah

2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Evolutionary studies conducted on NAC (NAM, ATAF1&2, and CUC2) genes for all major groups of land plants, indicate the presence of the NAC subfamilies, even in the early land plants. The varied roles played by NAC proteins in plant growth and development range from the formation of shoot apical meristem, floral organ development, reproduction, lateral shoot development, and defense responses to biotic and abiotic stresses. Considering the value and importance of solanaceous crops, the study of NAC proteins in these plants needs to be intensified. This will help to identify and functionally characterize their promoters, which will subsequently aid in engineering plants with improved performance under stressful conditions. In this review, the functionally characterized NAC transcription factors specific to tomato, potato, tobacco, chili pepper and eggplant (aubergine) are summarized, clearly indicating their biological functions in the defense mechanism of the plants, against biotic and abiotic stresses.

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Overexpression of a novel NAC-type tomato transcription factor, SlNAM1 , enhances the chilling stress tolerance of transgenic tobacco

Cited by 55 publications

References 45 publications

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress

Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

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