Overexpression of a pathway specific negative regulator enhances production of daunorubicin in bldA deficient Streptomyces peucetius ATCC 27952

Pokhrel, Anaya Raj; Chaudhary, Amit; Nguyen, Hue Thi; Dhakal, Dipesh; Le, Tuoi Thi; Shrestha, Anil; Liou, Kwangkyoung; Sohng, Jae Kyung

doi:10.1016/j.micres.2016.06.009

Cited by 19 publications

(11 citation statements)

References 40 publications

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“…Some examples of polyketides produced by Streptomyces are rapamycin (produced by Streptomyces hygroscopicus ), oleandomycin (produced by Streptomyces antibioticus ), actinorhodin (produced by Streptomyces coelicolor A3(2)), daunorubicin (produced by Streptomyces peucetius ) and caprazamycin (produced by Streptomyces sp. MK730-62F2) [19,20,21,22,23].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Polyketides in Streptomyces

2019

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Polyketides are a large group of secondary metabolites that have notable variety in their structure and function. Polyketides exhibit a wide range of bioactivities such as antibacterial, antifungal, anticancer, antiviral, immune-suppressing, anti-cholesterol, and anti-inflammatory activity. Naturally, they are found in bacteria, fungi, plants, protists, insects, mollusks, and sponges. Streptomyces is a genus of Gram-positive bacteria that has a filamentous form like fungi. This genus is best known as one of the polyketides producers. Some examples of polyketides produced by Streptomyces are rapamycin, oleandomycin, actinorhodin, daunorubicin, and caprazamycin. Biosynthesis of polyketides involves a group of enzyme activities called polyketide synthases (PKSs). There are three types of PKSs (type I, type II, and type III) in Streptomyces responsible for producing polyketides. This paper focuses on the biosynthesis of polyketides in Streptomyces with three structurally-different types of PKSs.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of Polyketides in Streptomyces

2019

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“…As expected, most of the gene clusters contain genes for transcriptional regulation, and interestingly 22 out of 42 gene clusters contain TTA codons. Among the six codons that encode leucine, UUA is very rare in Streptomyces as only 2–3% of the protein-coding genes contain TTA codons, which depend of the master regulator BldA, the only tRNA that read the UUA codon efficiently in Streptomyces genomes and responds to the secondary metabolisms 84,85 . Therefore, the presence of UUA codon suggests a highly‒regulated expression as part of the secondary metabolism.…”

Section: Resultsmentioning

confidence: 99%

Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery

Núñez-Montero

Lamilla

Abanto

et al. 2019

Sci Rep

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Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this study, the antimicrobial activity of organic extracts from Antarctic Streptomyces strains was evaluated by disk diffusion assays and minimum inhibitory concentration. The strain Streptomyces sp. So13.3 showed the greatest antibiotic activity (MIC = 15.6 μg/mL) against Gram-positive bacteria and growth reduction of Gram‒negative pathogens. The bioactive fraction in the crude extract was revealed by TLC‒bioautography at R f = 0.78 with molecular weight between 148 and 624 m/z detected by LC-ESI-MS/MS. The strain So13.3 was taxonomically affiliated as Streptomyces fildesensis . Whole genome sequencing and analysis suggested a 9.47 Mb genome size with 42 predicted biosynthetic gene clusters (BGCs) and 56 putative clusters representing a 22% of total genome content. Interestingly, a large number of them (11 of 42 BGCs and 40 of 56 putative BGCs), did not show similarities with other known BGCs. Our results highlight the potential of the Antarctic Streptomyces strains as a promising source of novel antimicrobials, particularly the strain Streptomyces fildesensis So13.3, which first draft genome is reported in this work.

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“…It can control the expression of phenotypic carB gene in S. coelicolor [130] and the heterologous expression of pur BGC from Streptomyces alboniger in S. lividans [131]. The interaction between bldA-tRNA and DnrO led to increasing the daunorubicin production in S. peucetius [8]. Some natural products were produced by the heterologous expression of BGCs involving tRNA synthases genes; for instance, the valanimycin BGC consists of the gene vlmL encoding Ser-tRNA Ser that produces seryl-tRNA, which may take part in the biosynthetic pathway of valanimycin [132].…”

Section: Trna Engineeringmentioning

confidence: 99%

“…Some natural secondary metabolites from Streptomyces are shown in Figure 1. [2,7], doxorubicin and geosmin from Streptomyces peucetius ATCC 27952 [8,9], oxazolomycin from Streptomyces albus JA3453 [10], germicidin from Streptomyces lividans TK24 [11], isoafricanol from Streptomyces malaysiensis [4], echinomycin from Streptomyces lasaliensis ATCC 35851 [12], bosamycin from Streptomyces sp. 120454 [3], cyclizidine from Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Heterologous Biosynthesis of Natural Products from Streptomyces

et al. 2021

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Streptomyces is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mining approaches demonstrate that many silent and cryptic biosynthetic gene clusters (BGCs) and many secondary metabolites are produced in very low amounts under laboratory conditions. One strain many compounds (OSMAC), overexpression/deletion of regulatory genes, ribosome engineering, and promoter replacement have been utilized to activate or enhance the production titer of target compounds. Hence, the heterologous expression of BGCs by transferring to a suitable production platform has been successfully employed for the detection, characterization, and yield quantity production of many secondary metabolites. In this review, we introduce the systematic approach for the heterologous production of secondary metabolites from Streptomyces in Streptomyces and other hosts, the genome analysis tools, the host selection, and the development of genetic control elements for heterologous expression and the production of secondary metabolites.

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Overexpression of a pathway specific negative regulator enhances production of daunorubicin in bldA deficient Streptomyces peucetius ATCC 27952

Cited by 19 publications

References 40 publications

Biosynthesis of Polyketides in Streptomyces

Biosynthesis of Polyketides in Streptomyces

Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery

Recent Advances in the Heterologous Biosynthesis of Natural Products from Streptomyces

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