Overexpression of a Rice TIFY Gene Increases Grain Size through Enhanced Accumulation of Carbohydrates in the Stem

Hakata, Makoto; Kuroda, Masaharu; Ohsumi, Akihiro; Hirose, Tatsuro; Nakamura, Hidemitsu; Muramatsu, M; Ichikawa, Hiroaki; Yamakawa, Hiromoto

doi:10.1271/bbb.120545

Cited by 44 publications

(36 citation statements)

References 40 publications

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“…In addition, cytokinin antagonizes ABA-mediated inhibition of cotyledon greening by promoting the degradation of ABI5 protein in Arabidopsis (Guan et al, 2014). Jasmonate (JA) is another important phytohormone (Hakata et al, 2012;Ishiga et al, 2013;Toda et al, 2013a,b), which regulates diverse aspects of plant developmental and environmental responses, including stamen development, root growth, anthocyanin accumulation, trichome initiation and plant defense against pathogens and insects (Franceschi & Grimes, 1991;Staswick et al, 1992;McConn & Browse, 1996;Sanders et al, 2000;Stintzi & Browse, 2000;Wasternack, 2007;Howe & Jander, 2008;Kazan & Manners, 2008;Browse, 2009;Cheng et al, 2009;Chung & Howe, 2009;Shan et al, 2009Shan et al, , 2011Pauwels et al, 2010). In recent decades, studies in Arabidopsis have revealed a JA signaling framework.…”

Section: Introductionmentioning

confidence: 99%

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

et al. 2019

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Summary Appropriate regulation of crop seed germination is of significance for agriculture production. In this study, we show that TaJAZ1, most closely related to Arabidopsis JAZ3, negatively modulates abscisic acid (ABA)‐inhibited seed germination and ABA‐responsive gene expression in bread wheat. Biochemical interaction assays demonstrate that the C‐terminal part containing the Jas domain of TaJAZ1 physically interacts with TaABI5. Similarly, Arabidopsis jasmonate‐ZIM domain (JAZ) proteins also negatively modulate ABA responses. Further we find that a subset of JAZ proteins could interact with ABI5 using the luciferase complementation imaging assays. Choosing JAZ3 as a representative, we demonstrate that JAZ3 interacts with ABI5 in vivo and represses the transcriptional activation activity of ABI5. ABA application could abolish the enrichment of JAZ proteins in the ABA‐responsive gene promoter. Furthermore, we find that ABA application could induce the expression of jasmonate (JA) biosynthetic genes and then increase the JA concentrations partially dependent on the function of ABI5, consequently leading to the degradation of JAZ proteins. This study sheds new light on the crosstalk between JA and ABA in modulating seed germination in bread wheat and Arabidopsis.

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Section: Introductionmentioning

confidence: 99%

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

et al. 2019

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“…OsTIFY3/OsJAZ1 is involved in spikelet development through interacting with OsCOI1b and OsMYC2 (Cai et al, 2014). Another rice JAZ gene, OsTIFY11b/OsJAZ10 could increase grain-size by enhancing accumulation of carbohydrates in the stem (Hakata et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of TIFY family genes in Moso Bamboo (Phyllostachys edulis) and expression profiling analysis under dehydration and cold stresses

Huang

Jin

Guo

et al. 2016

PeerJ

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The proteins containing the TIFY domain belong to a plant-specific family of putative transcription factors and could be divided into four subfamilies: ZML, TIFY, PPD and JAZ. They not only function as key regulators of jasmonate hormonal response, but are also involved in responding to abiotic stress. In this study, we identified 24 TIFY genes (PeTIFYs) in Moso bamboo (Phyllostachys edulis) of Poaceae by analyzing the whole genome sequence. One PeTIFY belongs to TIFY subfamily, 18 and five belong to JAZ and ZML subfamilies, respectively. Two equivocal gene models were re-predicted and a putative retrotransposition event was found in a ZML protein. The distribution and conservation of domain or motif, and gene structure were also analyzed. Phylogenetic analysis with TIFY proteins of Arabidopsis and Oryza sativa indicated that JAZ subfamily could be further divided to four groups. Evolutionary analysis revealed intragenomic duplication and orthologous relationship between P. edulis, O. sativa, and B. distachyon. Calculation of the non-synonymous (Ka) and synonymous (Ks) substitution rates and their ratios indicated that the duplication of PeTIFY may have occurred around 16.7 million years ago (MYA), the divergence time of TIFY family among the P. edulis-O. sativa, P. edulis-B. distachyon, and O. sativa-B. distachyon was approximately 39 MYA, 39 MYA, and 45 MYA, respectively. They appear to have undergone extensive purifying selection during evolution. Transcriptome sequencing revealed that more than 50% of PeTIFY genes could be up-regulated by cold and dehydration stresses, and some PeTIFYs also share homology to know TIFYs involved in abiotic stress tolerance. Our results made insights into TIFY family of Moso bamboo, an economically important non-timber forest resource, and provided candidates for further identification of genes involved in regulating responses to abiotic stress.

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“…It has been well suggested that TIFY genes play important roles in the jasmonate (JA) signaling pathway [16], [17], plant growth and development[18]–[21], and pathogen responses [22]–[24]. For example, in Arabidopsis , TIFY genes are suggested to negatively regulate the key transcriptional activator of JA responses [25], such as MYC2 [26], MYC3, MYC4 [26], [27], MYB21, MYB24 [28], bHLH017 and bHLH003 [29].…”

Section: Introductionmentioning

confidence: 99%

The Positive Regulatory Roles of the TIFY10 Proteins in Plant Responses to Alkaline Stress

Zhu

Liu

et al. 2014

PLoS ONE

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The TIFY family is a novel plant-specific protein family, and is characterized by a conserved TIFY motif (TIFF/YXG). Our previous studies indicated the potential roles of TIFY10/11 proteins in plant responses to alkaline stress. In the current study, we focused on the regulatory roles and possible physiological and molecular basis of the TIFY10 proteins in plant responses to alkaline stress. We demonstrated the positive function of TIFY10s in alkaline responses by using the AtTIFY10a and AtTIFY10b knockout Arabidopsis, as evidenced by the relatively lower germination rates of attify10a and attify10b mutant seeds under alkaline stress. We also revealed that ectopic expression of GsTIFY10a in Medicago sativa promoted plant growth, and increased the NADP-ME activity, citric acid content and free proline content but decreased the MDA content of transgenic plants under alkaline stress. Furthermore, expression levels of the stress responsive genes including NADP-ME, CS, H+-ppase and P5CS were also up-regulated in GsTIFY10a transgenic plants under alkaline stress. Interestingly, GsTIFY10a overexpression increased the jasmonate content of the transgenic alfalfa. In addition, we showed that neither GsTIFY10a nor GsTIFY10e exhibited transcriptional activity in yeast cells. However, through Y2H and BiFc assays, we demonstrated that GsTIFY10a, not GsTIFY10e, could form homodimers in yeast cells and in living plant cells. As expected, we also demonstrated that GsTIFY10a and GsTIFY10e could heterodimerize with each other in both yeast and plant cells. Taken together, our results provided direct evidence supporting the positive regulatory roles of the TIFY10 proteins in plant responses to alkaline stress.

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Overexpression of a Rice TIFY Gene Increases Grain Size through Enhanced Accumulation of Carbohydrates in the Stem

Cited by 44 publications

References 40 publications

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

JAZ proteins modulate seed germination through interaction with ABI5 in bread wheat and Arabidopsis

Genome-wide identification and characterization of TIFY family genes in Moso Bamboo (Phyllostachys edulis) and expression profiling analysis under dehydration and cold stresses

The Positive Regulatory Roles of the TIFY10 Proteins in Plant Responses to Alkaline Stress

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