Overexpression of a specific soybean GmGSTU4 isoenzyme improves diphenyl ether and chloroacetanilide herbicide tolerance of transgenic tobacco plants

Benekos, Kostantinos; Kissoudis, Christos; Nianiou-Obeidat, Irini; Labrou, Nikolaos E.; Madesis, Panagiotis; Kalamaki, Mary S.; Makris, Antonis; Tsaftaris, Athanasios

doi:10.1016/j.jbiotec.2010.07.011

Cited by 102 publications

(60 citation statements)

References 58 publications

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“…In a like manner, the GST activity in vitro of maize treated by R-isomer or R-28725 also showed a significant increase. These findings are in agreement with earlier reports (Benekos et al, 2010). Table 2 Kinetic parameters of GST.…”

Section: Glutathione S-transferases (Gst) Activitysupporting

confidence: 93%

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Fei

Cao

et al. 2016

Zemdirbyste-Agriculture

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Herbicide safener, a diverse group of chemicals, is an important tool used to protect plants from herbicidal injury. With the aim of decreasing drift injury of haloxyfop-P-methyl to sensitive plants, the protective effect of four safeners (R-28725, 3-dichloroacetyl oxazolidine and its two optical isomers) was evaluated. Physiological and biochemical tests were conducted under laboratory conditions in Northeast Agricultural University, China, by using seed treatment with safener and soil treatment with haloxyfop-P-methyl, respectively. The maize seeds treated with these safeners were safe from haloxyfop-P-methyl treatment. A positive correlation between growth level and endogenous glutathione (GSH) content, glutathione S-transferases (GST) activity was observed in this research. Enhancement of GSH content, GST activity and affinity of GST to 1-chloro-2,4-dinitrobenzene (CDNB) in maize treated by R-28725 was maximum. However, the detoxification of herbicide was not accompanied by the increase of acetyl-CoA-carboxylase (ACC) activity in maize.

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Section: Glutathione S-transferases (Gst) Activitysupporting

confidence: 93%

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Fei

Cao

et al. 2016

Zemdirbyste-Agriculture

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“…In particular, the application of R-28725 and imazethapyr increased GST activity in vivo by 144.20% and the application of R-stereoisomer and imazethapyr increased GST activity in vitro by 40.22% (Table 3). These findings are in agreement with an earlier report (Benekos et al, 2010).…”

Section: Gst Activitysupporting

confidence: 94%

3-Dichloroacetyl oxazolidine protect maize from imazethapyr herbicide injury

Zhao

et al. 2016

Chilean J. Agric. Res.

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Safeners are an important tool used to ensure the safe using of herbicide. The objective of this paper was to investigate the protective effect of four 3-dichloroacetyl oxazolidine safeners (3-dichloroacetyl-2,2-dimethyl-1,3-oxazolidine [R-28725], racemate of 3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine, and its two chiral stereoisomers) in reducing the injury caused by imazethapyr. Physiological and biochemical tests were conducted under laboratory condition, by using seed treatment with safeners and soil treatment with imazethapyr, respectively. The interaction of two safeners (R-28725 and R-stereoisomer) and imazethapyr reduced the injury of maize significantly, and also increased glutathione content, activity of glutathione S-transferases, and activity of acetolactate synthase in maize. When induced by R-stereoisomer, the GSH content in root and in shoot increased 100.7% and 73.6%, respectively. When induced by R-28725, the GST activity in vivo increased threefold and the GST activity in vitro more than doubled. The kinetic parameter Vmax of GST in the maize treated with R-28725 and R-stereoisomer increased by 102.2% and 81.9%, respectively, compared with the control. The results also showed that R-28725 and R-stereoisomer induced glutathione S-transferases affinity for the substrate of conjugation reaction significantly.

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“…These characteristics allow vascular plants to adapt well to land living. Tau GSTs play important roles in plant detoxification metabolism, stress tolerance, and light signaling (Loyall et al, 2000;Thom et al, 2002;Benekos et al, 2010;Jha et al, 2011). The origin and expansion of tau GSTs in vascular plants might be correlated with their broad adaptations to land living.…”

Section: Discussionmentioning

confidence: 99%

“…Genome-wide analysis of biochemical characteristics of Arabidopsis (Arabidopsis thaliana) and poplar (Populus trichocarpa) tau and phi GSTs found that these two classes of GSTs have broad substrate specificities (Dixon et al, 2009;Lan et al, 2009), which may be related to the high tolerance to abiotic stresses, especially to a broad spectrum of xenobiotics. Overexpression of tau or phi GSTs increases tolerance to herbicides, salt, and UV stressors in transgenic plants (Karavangeli et al, 2005;Benekos et al, 2010;Jha et al, 2011), indicating a protective role against abiotic stress. Recently, Arabidopsis GSTF2 was found to selectively bind the indole-derived phytoalexin camalexin and the flavonol quercetin-3-O-rhamnoside, suggesting a role in regulating the binding and transport of defense-related compounds in plants (Dixon et al, 2011).…”

mentioning

confidence: 99%

Functional Divergence of the Glutathione S-Transferase Supergene Family in Physcomitrella patens Reveals Complex Patterns of Large Gene Family Evolution in Land Plants

et al. 2012

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Plant glutathione S-transferases (GSTs) are multifunctional proteins encoded by a large gene family that play major roles in the detoxification of xenobiotics and oxidative stress metabolism. To date, studies on the GST gene family have focused mainly on vascular plants (particularly agricultural plants). In contrast, little information is available on the molecular characteristics of this large gene family in nonvascular plants. In addition, the evolutionary patterns of this family in land plants remain unclear. In this study, we identified 37 GST genes from the whole genome of the moss Physcomitrella patens, a nonvascular representative of early land plants. The 37 P. patens GSTs were divided into 10 classes, including two new classes (hemerythrin and iota). However, no tau GSTs were identified, which represent the largest class among vascular plants. P. patens GST gene family members showed extensive functional divergence in their gene structures, gene expression responses to abiotic stressors, enzymatic characteristics, and the subcellular locations of the encoded proteins. A joint phylogenetic analysis of GSTs from P. patens and other higher vascular plants showed that different class GSTs had distinct duplication patterns during the evolution of land plants. By examining multiple characteristics, this study revealed complex patterns of evolutionary divergence among the GST gene family in land plants.

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Overexpression of a specific soybean GmGSTU4 isoenzyme improves diphenyl ether and chloroacetanilide herbicide tolerance of transgenic tobacco plants

Cited by 102 publications

References 58 publications

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

3-Dichloroacetyl oxazolidine protect maize from imazethapyr herbicide injury

Functional Divergence of the Glutathione S-Transferase Supergene Family in Physcomitrella patens Reveals Complex Patterns of Large Gene Family Evolution in Land Plants

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