Overexpression of a Trichoderma HSP70 gene increases fungal resistance to heat and other abiotic stresses

Montero-Barrientos, Marta; Hermosa, Rosa; Nicolás, Carlos; Cardoza, Rosa E.; Gutiérrez, Santiago; Monte, Enrique

doi:10.1016/j.fgb.2008.09.003

Cited by 74 publications

(41 citation statements)

References 60 publications

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“…In T. reesei the transcription of HSP70 was strongly induced under anaerobic conditions (911). The overexpression of the HSP70 gene of T. harzianum resulted in a higher tolerance to oxidative, osmotic, and salt stress factors after heat pretreatment of the conidia (912). Furthermore, the product of the same gene under the regulation of a 35S promoter conferred more heat and stress tolerance when transferred to Arabidopsis thaliana (913).…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

166

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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Section: Heat Shock Proteinsmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

166

195

View full text Add to dashboard Cite

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“…The hsp70 gene from T. harzianum T34 was cloned and characterized (ManteroBarrientos et al, 2008) and encoding protein expression in Arabidopsis showed higher tolerance to heat and other abiotic stresses (Mantero-Barrientos, et al, 2008). The encoding protein product of this gene facilitates higher level of fungal resistance to heat and other stresses such as osmotic, salt and oxidative tolerances.…”

Section: Biotic and Abiotic Stress Tolerancementioning

confidence: 99%

Trichoderma: A significant fungus for agriculture and environment

Waghunde¹,

Shelake²,

Sabalpara³

2016

Afr. J. Agric. Res.

144

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The novel technologies in all areas of agriculture have improved agricultural production, but some modern practices affect the environment. The recent challenge faced by advanced farming is to achieve higher yields in environment-friendly manner. Thus, there is an immediate need to find eco-friendly solutions such as wider application of biocontrol agents. Among various types of species being used as biocontrol agents, including fungi and bacteria, fungal genus Trichoderma produces different kinds of enzymes which play a major role in biocontrol activity like degradation of cell wall, tolerance to biotic or abiotic stresses, hyphal growth etc. The understanding of filamentous fungi belonging to the genus Trichoderma has continuously evolved since last two decades, from the simple concepts of biocontrol agents to their recently established role as symbionts with different beneficial effects to the plants. Recent findings from structural and functional genomics approaches suggest the additional use of these microbes as model to study mechanisms involved in multiple player interactions that is, microbes-microbes-plant-environment. In this work, historical development of Trichoderma spp., mode of action against different biological agents, potential applications and recent mass production techniques are summarized and discussed in detail with updated advances with their application in the agriculture and sustainable environment.

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“…nox1 cDNA was amplified by PCR with the primer pair X5 and X3 and cloned to the pGEM-T Easy vector. After digestion with XbaI, the 1.8-kb fragment corresponding to the nox1 cDNA was ligated to the pLMG vector (36), previously digested with XbaI. The nox1 expression cassette of 3.6 kb, which contains the gpdA (glyceraldehyde 3-phosphate dehydrogenase) gene promoter from A. nidulans and the cbh2 (cellobiohydrolase II) termination region from Trichoderma reesei, was isolated by digestion with HindIII and cloned into plasmid pJL43b1.…”

Section: Microorganismsmentioning

confidence: 99%

Functional Analysis of the Trichoderma harzianum nox1 Gene, Encoding an NADPH Oxidase, Relates Production of Reactive Oxygen Species to Specific Biocontrol Activity against Pythium ultimum

Montero-Barrientos

Hermosa

Cardoza

et al. 2011

Appl Environ Microbiol

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The synthesis of reactive oxygen species (ROS) is one of the first events following pathogenic interactions in eukaryotic cells, and NADPH oxidases are involved in the formation of such ROS. The nox1 gene of Trichoderma harzianum was cloned, and its role in antagonism against phytopathogens was analyzed in nox1-overexpressed transformants. The increased levels of nox1 expression in these transformants were accompanied by an increase in ROS production during their direct confrontation with Pythium ultimum. The transformants displayed an increased hydrolytic pattern, as determined by comparing protease, cellulase, and chitinase activities with those for the wild type. In confrontation assays against P. ultimum the nox1-overexpressed transformants were more effective than the wild type, but not in assays against Botrytis cinerea or Rhizoctonia solani. A transcriptomic analysis using a Trichoderma high-density oligonucleotide (HDO) microarray also showed that, compared to gene expression for the interaction of wild-type T. harzianum and P. ultimum, genes related to protease, cellulase, and chitinase activities were differentially upregulated in the interaction of a nox1-overexpressed transformant with this pathogen. Our results show that nox1 is involved in T. harzianum ROS production and antagonism against P. ultimum.

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Overexpression of a Trichoderma HSP70 gene increases fungal resistance to heat and other abiotic stresses

Cited by 74 publications

References 60 publications

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Trichoderma: A significant fungus for agriculture and environment

Functional Analysis of the Trichoderma harzianum nox1 Gene, Encoding an NADPH Oxidase, Relates Production of Reactive Oxygen Species to Specific Biocontrol Activity against Pythium ultimum

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