Overexpression of an aquaporin gene EsPIP1;4 enhances abiotic stress tolerance and promotes flowering in Arabidopsis thaliana

Yang, Xiaomin; Li, Jiawen; Ji, Chengcheng; ZhaoXin, Wei; Zhao, Tong; Pang, Qiuying

doi:10.1016/j.plaphy.2022.10.019

Cited by 11 publications

(7 citation statements)

References 55 publications

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“…RD22 is up-regulated in drought conditions and plays a crucial role in the stomatal closure 50 . PIP1;4 is a water channel (aquaporin) important role in drought tolerance 51 . It is also known that the down-regulation or disruption of the LHCB2.3 protein decreases drought tolerance in Arabidopsis 52 .…”

Section: Discussionmentioning

confidence: 99%

Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet

Kumar,

Singh,

Kumar Singh

et al. 2023

Journal of Experimental Botany

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Climate change inflicts several stresses on plants, of which dehydration stress severely affects growth and productivity. C4 plants possess better adaptability to dehydration stress; however, the role of epigenetic modifications underlying this trait is unclear. Particularly, the molecular links between histone modifiers and their regulation remain elusive. In this study, genome-wide H3K9 acetylation (H3K9ac) enrichment using ChIP-seq was performed in two foxtail millet cultivars contrastingly differing in dehydration tolerance (IC403579; cv. IC4 – tolerant, and IC480117; cv. IC41 – sensitive). It revealed that a histone deacetylase, SiHDA9, was significantly up-regulated in the sensitive cultivar. Further characterization indicated that SiHDA9 interacts with SiHAT3.1 and SiHDA19 to form a repressor complex. SiHDA9 might be recruited through the SiHAT3.1 recognition sequence onto the upstream of dehydration-responsive genes to decrease H3K9 acetylation levels. The silencing of SiHDA9 resulted in the up-regulation of crucial genes, namely, SiRAB18, SiRAP2.4, SiP5CS2, SiRD22, SiPIP1;4 and SiLHCB2.3, which imparted dehydration tolerance in the sensitive cultivar (IC41). Overall, the study provides mechanistic insights into SiHDA9-mediated regulation of dehydration stress response in foxtail millet.

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Section: Discussionmentioning

confidence: 99%

Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet

Kumar,

Singh,

Kumar Singh

et al. 2023

Journal of Experimental Botany

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“…AQPs exhibit functional diversity and are widely distributed among various plant species. Among them, the plasma membrane intrinsic protein (PIP) subgroups play vital roles in ion transport, seed germination, root growth, flowering promotion, and abiotic stress tolerance, such as tolerance to salt, cold, or drought [5,15]. The transport activity and substrate selection of AQPs are influenced by NPA motifs, Froger's positions, and the ar/R selectivity filter.…”

Section: Discussionmentioning

confidence: 99%

“…The PIP subgroup further divides into the PIP1 and PIP2 categories. PIP1 members have a long N-terminus and short C-terminal tails, while PIP2 variants have a short N-terminus and a longer C-terminus [5].…”

Section: Introductionmentioning

confidence: 99%

Expression and Functional Analysis of the PaPIP1-2 Gene during Dormancy and Germination Periods of Kernel-Using Apricot (Prunus armeniaca L.)

Li,

Zheng,

Wang

et al. 2023

Forests

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Aquaporins play a crucial role in helping water molecules move across cell membranes. While some studies have examined the role of AQPs in model plants like Arabidopsis, their impact on the ability of non-model plants to withstand environmental stress is largely unknown. In this study, we have explored the functions of the PaPIP1-2 gene, which encodes a protein called PIP, in apricot kernels (Prunus armeniaca L.). Our findings reveal how the PaPIP1-2 gene behaves during both dormancy and sprouting phases. Using a network analysis, we identified its interaction with six genes related to cold resistance. The mRNA levels of PaAQP genes, which co-express with cold resistance genes, remain consistent throughout different stages of P. armeniaca flower bud development, including physiological dormancy (PD), ecological dormancy (ED), sprouting period (SP), and germination stage (GS). Furthermore, our investigation of the location of the GFP-tagged PaPIP1-2 protein showed that it is mainly found in the cell membrane. Yeast strains with overexpressed PaPIP1-2 exhibited improved cold resistance and higher protein content. Similarly, when we overexpressed PaPIP1-2 in Arabidopsis, it enhanced the growth of these transgenic plants under cold stress. This improvement was associated with reduced levels of MDA (malondialdehyde); decreased ion leakage; increased proline accumulation; superoxide dismutase (SOD) activity; and the expression of cold resistance genes like AtPUB26, AtBTF3L, AtEBF1-1, and AtRAV1, compared with the wild-type plants. In summary, our results highlight the role of the P. armeniaca PaPIP1-2 gene in enhancing cold resistance and its importance in the dormancy and germination stages.

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“…Generally, overexpression of these PIP1 and PIP2 aquaporins enhances root, stem and leaves growth and development (Chen et al 2022). Moreover, PIP aquaporins are also involved in reproductive organ development such as owering, fruit and seed formation (Chen et al 2022;Yang et al 2022). Different PIP2 are up-regulated or down-regulated according to fruit initiation or ripening (Zhu and Ming 2019).…”

Section: Introductionmentioning

confidence: 99%

Aquaporins-mediated water availability in substrates for cannabis cultivation in relation to CBD yield.

Ortiz-Delvasto¹,

García-Gómez²,

Carvajal³

et al. 2023

Preprint

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Aims The objective of this study was to identify the most suitable substrate for Cannabis sativa L. cultivation based on its effects on water relations and CBD production. Methods Biomass production, physiological parameters, mineral contents, changes in the expression levels of the PIP aquaporins and the concentration of CBD was measured in Cannabis sativa L. (var. Tiborszallasi) plants cultivated on 5 substrates with different physical-chemical characteristic under controlled conditions. Results The substrates available water (AW) was the main factor affecting growth and production. The efficiency of the water use was governed fundamentally by transpiration. S1 and S3 were those in which the plants grew optimally and allows plants to invest energy in secondary metabolites production acquiring high levels of CBD. The plants grown in S2 and S5, composed by coconut fiber and perlite, showed the lowest growth in agreement with low transpiration rates which reduce the water uptake. S5 substrate, with some AW still available, is forcing plants to invest energy in improve water and nutrients transport as observed by the high levels of nutrients in planta and high PIPs expression levels. S4 plants presented the highest inflorescences production and CBD content which can be attributed to plant stress due to the low levels of AW and high pH and electrical conductivity (EC). Conclusion The absorption of water and minerals by plants has been affected by PIP-mediated water transport, playing key roles for an optimal use of the water present in the substrates with specific isoforms involved in this responses.

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Overexpression of an aquaporin gene EsPIP1;4 enhances abiotic stress tolerance and promotes flowering in Arabidopsis thaliana

Cited by 11 publications

References 55 publications

Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet

Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet

Expression and Functional Analysis of the PaPIP1-2 Gene during Dormancy and Germination Periods of Kernel-Using Apricot (Prunus armeniaca L.)

Aquaporins-mediated water availability in substrates for cannabis cultivation in relation to CBD yield.

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