Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance

Deng, Wei; Luo, Keming; Li, De-Mou; Zheng, Xuelian; Wei, Xiaoyang; Smith, W. K.; Thammina, Chandra; Lu, Litang; Li, Yang; Pei, Yan

doi:10.1093/jxb/erl201

Cited by 114 publications

(61 citation statements)

References 48 publications

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“…This phenomenon is not yet fully understood. However, from the available information it may be concluded that the observed competition for uptake is a consequence of the lack of specificity of the individual uptake systems for each cation (Gardner 2003;Shaul 2002;Deng et al 2006;Marschner 2012). Some Mg transporters may also transport other cations like K. As a consequence a high plant available K concentration in the soil/rhizosphere blocks these unspecific Mg transporters for Mg uptake.…”

Section: Mechanisms Of Mg Uptake By Cropsmentioning

confidence: 99%

Magnesium mobility in soils as a challenge for soil and plant analysis, magnesium fertilization and root uptake under adverse growth conditions

2012

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Background Due to its unique chemistry magnesium (Mg) is subject to various cycling processes in agricultural ecosystems. This high mobility of Mg needs to be considered for crop nutrition in sustainable agricultural systems. The Mg mobility in soils and plants and its consequences for crop nutrition are understood, but recent findings in crop Mg uptake, translocation and physiology particularly under adverse growth conditions give new insights into the importance of Mg in crop production. Scope The aim of this review is to combine the knowledge on the origin and mobility of Mg in soils with the role of Mg in plant stress physiology and recent evidence on the principles of crop Mg uptake. The question is addressed whether the progress made in Mg research, particularly on the role of Mg in stress physiology, makes a revision of the development of Mg fertilization recommendations necessary. Conclusions New insights into Mg uptake and utilization but particularly into the role of Mg in increasing crop tolerance to various stresses indicate changes in the crop Mg demand under adverse growth conditions. Future work should incorporate these findings in optimization of site-specific balanced fertilization programs particularly under stress conditions.

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Section: Mechanisms Of Mg Uptake By Cropsmentioning

confidence: 99%

Magnesium mobility in soils as a challenge for soil and plant analysis, magnesium fertilization and root uptake under adverse growth conditions

2012

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“…This Al-induced Mg deficiency has been suggested as a primary cause of Al toxicity (MacDiarmid & Gardner, 1996). Support for this hypothesis comes from research where overexpression of yeast and bacterial Mg transporters in yeast increased Mg (Co 2+ ) uptake and improved Al tolerance significantly (Fowler et al, 1999) and the more recent findings that overexpression of an Arabidopsis magnesium transport gene in Nicotiana benthamiana increased Mg uptake in the presence of Al and conferred Al tolerance (Deng et al, 2006).…”

Section: Protective Effect Of Magnesium On Distinct Root Classes Of 1mentioning

confidence: 96%

“…MacDiarmid & Gardner (1996) also reported that the toxic effect of Al 3+ on Sacharomyces cerevisae was mitigated by Mg supplementation, unlike similar additions of Ca to the growth medium. Furthermore, it was recently reported that the overexpression of an Arabidopsis magnesium transport gene in Nicotiana benthamiana increased Mg uptake in the presence of Al and conferred Al tolerance (Deng et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Timing, location and crop species influence the magnitude of amelioration of aluminum toxicity by magnesium

Silva

Corrêa

Novais

et al. 2009

Rev. Bras. Ciênc. Solo

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The protective effect of cations, especially Ca and Mg, against aluminum (Al) rhizotoxicity has been extensively investigated in the last decades. The mechanisms by which the process occurs are however only beginning to be elucidated. Six experiments were carried out here to characterize the protective effect of Mg application in relation to timing, location and crop specificity: Experiment 1 - Protective effect of Mg compared to Ca; Experiment 2 - Protective effect of Mg on distinct root classes of 15 soybean genotypes; Experiment 3 - Effect of timing of Mg supply on the response of soybean cvs. to Al; Experiment 4 - Investigating whether the Mg protective effect is apoplastic or simplastic using a split-root system; Experiment 5 - Protective effect of Mg supplied in solution or foliar spraying, and Experiment 6 - Protective effect of Mg on Al rhizotoxicity in other crops. It was found that the addition of 50 mmol L-1 Mg to solutions containing toxic Al increased Al tolerance in 15 soybean cultivars. This caused soybean cultivars known as Al-sensitive to behave as if they were tolerant. The protective action of Mg seems to require constant Mg supply in the external medium. Supplying Mg up to 6 h after root exposition to Al was sufficient to maintain normal soybean root growth, but root growth was not recovered by Mg addition 12 h after Al treatments. Mg application to half of the root system not exposed to Al was not sufficient to prevent Al toxicity on the other half exposed to Al without Mg in rooting medium, indicating the existence of an external protection mechanism of Mg. Foliar spraying with Mg also failed to decrease Al toxicity, indicating a possible apoplastic role of Mg. The protective effect of Mg appeared to be soybean-specific since Mg supply did not substantially improve root elongation in sorghum, wheat, corn, cotton, rice, or snap bean when grown in the presence of toxic Al concentrations.

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“…AtMGT2-10/AtMGT1 functionally complemented a bacterial mutant lacking Mg transport capability and was localized to the plasma membrane (Li et al 2001). Overexpression of AtMGT2-10/AtMGT1 was found to contribute to tolerance to low levels of Mg in external solution (Deng et al 2006). One other gene, AtMHX, has been identified as a vacuolar Mg 2þ /H þ exchanger in Arabidopsis: mRNA of AtMHX is highly accumulated at the xylem parenchyma where the cells may play a key role in xylem loading and unloading (Shaul et al 1999).…”

Section: Introductionmentioning

confidence: 99%

The analysis of magnesium transport system from external solution to xylem in rice root

Tanoi

Saito

Iwata

et al. 2011

Soil Science and Plant Nutrition

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We report the characterization of magnesium (Mg) transport from external solution to xylem by using xylem fluid analysis in rice (Oryza sativa L. Nipponbare). It was indicated that Mg uptake and/or xylem loading was mediated by two transport systems, a saturable and a linear component. The K m value of the saturable component was approximately 20 mM, and the saturated concentration was calculated to be 1.5 mM. Using a multi-compartment transport box with excised roots, the transfer from external solution to xylem was shown to be inhibited by hexaamminecobalt(III) {[Co(III)(NH 3 ) 6 ], Co-Hex}, a selective inhibitor of CorA-mediated influx of Mg 2þ , suggesting that the MRS2/MGT genes that belong to the superfamily of CorA-type membrane proteins are involved in the Mg transport system.

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Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance

Cited by 114 publications

References 48 publications

Magnesium mobility in soils as a challenge for soil and plant analysis, magnesium fertilization and root uptake under adverse growth conditions

Magnesium mobility in soils as a challenge for soil and plant analysis, magnesium fertilization and root uptake under adverse growth conditions

Timing, location and crop species influence the magnitude of amelioration of aluminum toxicity by magnesium

The analysis of magnesium transport system from external solution to xylem in rice root

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