Overexpression of Arabidopsis Dehydration- Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Hwang, Jin-Woo; Lim, Chan Ju; Chen, Huan; Je, Jihyun; Song, Chieun; Lim, Chae Oh

doi:10.1007/s10059-012-2188-2

Cited by 56 publications

(39 citation statements)

References 28 publications

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“…A study of Hsf1's action on the circadian clock in mammalian cells was reported recently; however, the molecular mechanism remains unknown (44,45). In general, Hsfs have been described to target HSP genes via multiple HSE repeats in their promoters, and few studies exist where Hsfs regulate non-HSP genes (46)(47)(48). Importantly, mammalian Hsfs are known to control the expression of various developmental genes in addition to HSPs, and it recently has become clear that the stress-independent expression of HSF1 is crucial in oncogenesis (49, 50).…”

Section: Discussionmentioning

confidence: 99%

HsfB2b-mediated repression ofPRR7directs abiotic stress responses of the circadian clock

Kolmos

Chow

Pruneda‐Paz

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The circadian clock perceives environmental signals to reset to local time, but the underlying molecular mechanisms are not well understood. Here we present data revealing that a member of the heat shock factor (Hsf) family is involved in the input pathway to the plant circadian clock. Using the yeast one-hybrid approach, we isolated several Hsfs, including HEAT SHOCK FACTOR B2b (HsfB2b), a transcriptional repressor that binds the promoter of PSEUDO RESPONSE REGULATOR 7 (PRR7) at a conserved binding site. The constitutive expression of HsfB2b leads to severely reduced levels of the PRR7 transcript and late flowering and elongated hypocotyls. HsfB2b function is important during heat and salt stress because HsfB2b overexpression sustains circadian rhythms, and the hsfB2b mutant has a short circadian period under these conditions. HsfB2b is also involved in the regulation of hypocotyl growth under warm, short days. Our findings highlight the role of the circadian clock as an integrator of ambient abiotic stress signals important for the growth and fitness of plants.circadian clock | Hsf | heat compensation | salt tolerance T he circadian clock is an endogenous timing mechanism that ensures that daily rhythmic processes are synchronized with the environment. The circadian oscillator runs with a period of ∼24 h. It entrains to environmental signals, such as light and temperature, and in this way is able to anticipate daily environmental changes (1). Molecular genetics and modeling have revealed that the circadian system comprises several interconnected feedback loops involving multiple phase-specific gene products (2).In the model plant Arabidopsis, the morning feedback loops are composed of the major oscillator proteins CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), which peak in abundance at dawn and activate the expression of early-late morning genes PSEUDO RESPONSE REGULATOR 9 (PRR9) and PRR7 (3). Pseudoresponse regulator (PRR) proteins are transcriptional repressors, and PRR9 and PRR7 close the morning loops by binding to the CCA1 and LHY promoters (4). The transcription of PRR9 is acutely activated by light, a feature that is not shared by PRR7 (3, 5). Disruption of either locus results in a mild clock phenotype, including an increased clock period of ∼1 h and an elongated hypocotyl (6-8). The double loss-of-function mutant exhibits a strong synergistic phenotype with free-running period lengths of up to 35 h and loss of temperature entrainment and compensation, indicating overlapping roles for PRR9 and PRR7 (3, 9, 10).The ambient environment influences the circadian clock in a number of different ways. Although the most well-studied input cue is light, temperature is a major zeitgeber as well (11). Brief pulses (from minutes to hours, depending on the intensity) result in acute responses from the clock. Importantly, the severity of such responses is time-dependent and gated by the oscillator (12-14). Longer durations of zeitgeber change can function as an entrainment signal and general...

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Section: Discussionmentioning

confidence: 99%

HsfB2b-mediated repression ofPRR7directs abiotic stress responses of the circadian clock

Kolmos

Chow

Pruneda‐Paz

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Hwang et al (2012) also reported that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.…”

Section: Introductionmentioning

confidence: 93%

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Bouaziz

Jbir

Charfeddine

et al. 2014

Plant Cell Tiss Organ Cult

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We have shown previously that the potato dehydration responsive element binding (StDREB1) transcription factor plays an important role in regulating and improving salt and drought stress-response genes in potato plant. To further characterize StDREB1 involvement in stress response, we focuse here on the investigation of the StDREB1 target genes by an Electrophoretic mobility shift assay. The data obtains indicated that the StDREB1 protein can bind to both GCC and DRE boxes in the promoter sequence of target genes, suggesting that this transcription factor may play a key role in the response to abiotic-and biotic-stresses by the activation of the DRE-and GCCmediated signaling pathways. In a second step, since some DREB factors were related to the oxidative stress response, we showed that H 2 O 2 treatment led to a significant increase of StDREB1 expression in wild-type potato plants. Moreover, the analysis of the oxidative stress response of StDREB1 transgenic potato plants revealed lower levels of H 2 O 2 and malondialdehyde than wild-type control plants submitted in vitro to salt stress. An increase in the antioxidant enzyme activities including superoxide dismutase (SOD) and catalase was also observed in StDREB1 plants. In addition, an enhanced expression of the Cu/Zn SOD gene was noticed in these StDREB1 transgenic plants, cultivated under salt stress conditions. These results suggest that StDREB1 plays an essential role in the regulation of stress-response by regulating the oxidative stress response. The involvement of this transcription factor in the activation of osmoprotectant synthesis was also confirmed.

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“…Among others, we observe misregulation of RING MEMBRANE-ANCHOR 1 (RMA1), an uncharacterized protein with possible E3 ubiquitin ligase activity, and DREB2C, a member of the DREB subfamily A-2 of the ERF/ AP2 transcription factor family implicated in drought stress responses (Fig. 4 B-E) (46)(47)(48)(49). Interestingly, in addition to DREB2C, 39 other transcription factors identified as CCA1 targets in this study recently have been shown to interact with the CCA1 promoter in a yeast one-hybrid assay (Dataset S3) (50).…”

Section: Countmentioning

confidence: 99%

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Nagel

Doherty

Pruneda‐Paz

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

234

279

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The circadian clock in Arabidopsis exerts a critical role in timing multiple biological processes and stress responses through the regulation of up to 80% of the transcriptome. As a key component of the clock, the Myb-like transcription factor CIRCADIAN CLOCK ASSOCIATED1 (CCA1) is able to initiate and set the phase of clockcontrolled rhythms and has been shown to regulate gene expression by binding directly to the evening element (EE) motif found in target gene promoters. However, the precise molecular mechanisms underlying clock regulation of the rhythmic transcriptome, specifically how clock components connect to clock output pathways, is poorly understood. In this study, using ChIP followed by deep sequencing of CCA1 in constant light (LL) and diel (LD) conditions, more than 1,000 genomic regions occupied by CCA1 were identified. CCA1 targets are enriched for a myriad of biological processes and stress responses, providing direct links to clock-controlled pathways and suggesting that CCA1 plays an important role in regulating a large subset of the rhythmic transcriptome. Although many of these target genes are evening expressed and contain the EE motif, a significant subset is morning phased and enriched for previously unrecognized motifs associated with CCA1 function. Furthermore, this work revealed several CCA1 targets that do not cycle in either LL or LD conditions. Together, our results emphasize an expanded role for the clock in regulating a diverse category of genes and key pathways in Arabidopsis and provide a comprehensive resource for future functional studies.genome-wide | circadian clock | clock-controlled outputs | transcriptional regulation I n anticipation of the daily changes in light and temperature, the plant's internal circadian clock regulates a large portion of the transcriptome, nearly 80% in rice, poplar, and Arabidopsis (1, 2). The clock provides temporal coordination of multiple biological functions to ensure optimal efficiency. For example, before dawn, photosynthetic transcripts accumulate in anticipation of the expected sunlight. Even when shifted to constant light (LL) and temperature, in the absence of day/night cues, transcripts associated with photosynthesis are still up-regulated before the subjective day (1-4). Not only are light-and energyrelated activities restricted to particular times, but other aspects of plant growth, including water use, hormone activity, UVB response, and low-temperature response, show this gating effect (5-9). This temporal partitioning of biological responses ultimately provides an optimal integration of the plant's organismal functions with the environment.The circadian clock in plants involves a posttranscriptional component and a well-studied transcriptional-translation feedback regulation between the Myb-like transcription factors CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) and a member of the PSEUDO RESPONSE REGULATOR (PRR) family, TIMING OF CAB2 EXPRESSION 1 (TOC1) (10-16). Together these three components contribute t...

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Overexpression of Arabidopsis Dehydration- Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Cited by 56 publications

References 28 publications

HsfB2b-mediated repression ofPRR7directs abiotic stress responses of the circadian clock

HsfB2b-mediated repression ofPRR7directs abiotic stress responses of the circadian clock

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

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