Overexpression of Arabidopsis OPR3 in Hexaploid Wheat (Triticum aestivum L.) Alters Plant Development and Freezing Tolerance

Pigolev, A. V.; Miroshnichenko, Dmitry; Pushin, Alexander; Terentyev, Vasily V.; Boutanayev, Alexander M; Dolgov, Sergey; Savchenko, Tatyana

doi:10.3390/ijms19123989

Cited by 39 publications

(33 citation statements)

References 78 publications

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“…Tritici [ 23 ]. Overexpression of AtOPR3 in hexaploid wheat could enhance the wheat freezing tolerance [ 24 ]. Under the drought stress in rice, OsOPR7 expression level was upregulated to a peak within 0.5 h [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of OPR Family Genes in Cotton Identified a Role for GhOPR9 in Verticillium dahliae Resistance

Liu

Sun

Zhang

et al. 2020

Genes

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The 12-oxo-phytodienoic acid reductases (OPRs) have been proven to play a major role in plant development and growth. Although the classification and functions of OPRs have been well understood in Arabidopsis, tomato, rice, maize, and wheat, the information of OPR genes in cotton genome and their responses to biotic and abiotic stresses have not been reported. In this study, we found 10 and 9 OPR genes in Gossypium hirsutum and Gossypium barbadense, respectively. They were classified into three groups, based on the similar gene structure and conserved protein motifs. These OPR genes just located on chromosome 01, chromosome 05, and chromosome 06. In addition, the whole genome duplication (WGD) or segmental duplication events contributed to the evolution of the OPR gene family. The analyses of cis-acting regulatory elements of GhOPRs showed that the functions of OPR genes in cotton might be related to growth, development, hormone, and stresses. Expression patterns showed that GhOPRs were upregulated under salt treatment and repressed by polyethylene glycol 6000 (PEG6000). The expression patterns of GhOPRs were different in leaf, root, and stem under V. dahliae infection. GhOPR9 showed a higher expression level than other OPR genes in cotton root. The virus-induced gene silencing (VIGS) analysis suggested that knockdown of GhOPR9 could increase the susceptibility of cotton to V. dahliae infection. Furthermore, GhOPR9 also modulated the expressions of jasmonic acid (JA) pathway-regulated genes under the V. dahliae infection. Overall, our results provided the evolution and potential functions of the OPR genes in cotton. These findings suggested that GhOPR9 might play an important role in cotton resistance to V. dahliae.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of OPR Family Genes in Cotton Identified a Role for GhOPR9 in Verticillium dahliae Resistance

Liu

Sun

Zhang

et al. 2020

Genes

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“…Previous gene expression analysis demonstrated that overexpressing AtOPR3 selectively affected the expression of various genes of the endogenous jasmonate system, while the expression of other genes remained unaltered. Transgenic wheat plants with high AtOPR3 expression levels exhibited notably altered plant growth and development, including delayed germination, slower growth, and anther indehiscence [47]. These findings indicate that these plant phenotypes are regulated by direct or indirect interactions of these genes.…”

Section: Discussionmentioning

confidence: 89%

Comparative transcriptome analysis identifies jasmonic pathway genes related to anther dehiscence in Solanum melongena L.

Wang¹,

Zhang

Yuan

et al. 2020

Preprint

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Background: Anther indehiscence is an important form of functional male sterility that can facilitate the production of hybrid seeds. However, the molecular mechanisms of anther indehiscence-based male sterility in eggplant (Solanum melongena L.) have not been thoroughly explored. Therefore, we performed RNA-seq analysis to investigate the molecular mechanisms of anther dehiscence in eggplant. Results: We used transcriptome sequencing and qRT-PCR assays to compare the anthers of normally developing (F142) and anther indehiscent (S12) eggplant. We identified 2670 differentially expressed genes between lines. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified 31 differentially expressed genes related to hormone biosynthesis. We therefore measured the contents of the phytohormones jasmonic acid (JA), IAA, GA3 and ABA in S12 and F142. There were no significant differences in IAA, GA3 or ABA levels between S12 and F142. However, JA levels were significantly lower in S12 than in F142. Five key genes in the JA signaling pathway were differentially expressed in S12 vs. F142. Of these, SmJAZ and SmJAR-like were significantly up-regulated and SmDAD1, SmLOX and SmCOI1 were down-regulated in S12 vs. F142. In DNA-protein interaction studies, SmLOX, SmAOC, SmOPR3, SmCOI1 and SmJAZ1 failed to be enriched on the chromatin of SmDAD1. Protein–protein interaction studies identified a direct interaction between SmDAD1 and SmLOX, but SmDAD1 failed to interact with SmAOC, SmOPR3, SmCOI1 and SmJAZ1. Conclusion: JA is an essential factor affecting anther dehiscence in eggplant. SmDAD1 interacts with SmLOX to alter JA levels, thereby regulating anther dehiscence. Our data represent a valuable resource for further exploring the regulatory mechanisms underlying anther dehiscence in eggplant.

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“…The function of TaOPR2 in JA biosynthesis is regulated by various biotic and abiotic stresses (Wang et al 2016b). Transgenic wheat plants overexpressing AtOPR3 display altered developmental phenotypes, including delayed germination, growth retardation and delayed flowering time and senescence (Pigolev et al 2018). In bread wheat, the TaJAZ1 protein inhibits seed germination via ABA and the expression of ABA responsive genes by physically interacting with TaABI5 (Ju et al 2019).…”

Section: Discussionmentioning

confidence: 99%

A WRKY transcription factor, TaWRKY40‐D, promotes leaf senescence associated with jasmonic acid and abscisic acid pathways in wheat

et al. 2020

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Leaf senescence is a complex and precise regulatory process that is correlated with numerous internal and environmental factors. Leaf senescence is tightly related to the redistribution of nutrients, which significantly affects productivity and quality, especially in crops. Evidence shows that the mediation of transcriptional regulation by WRKY transcription factors is vital for the fine‐tuning of leaf senescence. However, the underlying mechanisms of the involvement of WRKY in leaf senescence are still unclear in wheat. Using RNA sequencing data, we isolated a novel WRKY transcription factor, TaWRKY40‐D, which localizes in the nucleus and is basically induced by the progression of leaf senescence. TaWRKY40‐D is a promoter of natural and dark‐induced leaf senescence in transgenic Arabidopsis thaliana and wheat. We also demonstrated a positive response of TaWRKY40‐D in wheat upon jasmonic acid (JA) and abscisic acid (ABA) treatment. Consistent with this, the detached leaves of TaWRKY40‐D VIGS (virus‐induced gene silencing) wheat plants showed a stay‐green phenotype, while TaWRKY40‐D overexpressing Arabidopsis plants showed premature leaf senescence after JA and ABA treatment. Moreover, our results revealed that TaWRKY40‐D positively regulates leaf senescence, possibly by altering the biosynthesis and signalling of JA and ABA pathway genes. Together, our results suggest a new regulator of JA‐ and ABA‐related leaf senescence, as well as a new candidate gene that can be used for molecular breeding in wheat.

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Overexpression of Arabidopsis OPR3 in Hexaploid Wheat (Triticum aestivum L.) Alters Plant Development and Freezing Tolerance

Cited by 39 publications

References 78 publications

Genome-Wide Analysis of OPR Family Genes in Cotton Identified a Role for GhOPR9 in Verticillium dahliae Resistance

Genome-Wide Analysis of OPR Family Genes in Cotton Identified a Role for GhOPR9 in Verticillium dahliae Resistance

Comparative transcriptome analysis identifies jasmonic pathway genes related to anther dehiscence in Solanum melongena L.

A WRKY transcription factor, TaWRKY40‐D, promotes leaf senescence associated with jasmonic acid and abscisic acid pathways in wheat

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