Overexpression of Arabidopsis ubiquitin ligase AtPUB46 enhances tolerance to drought and oxidative stress

Adler, Guy; Mishra, Amit Kumar; Maymon, Tzofia; Raveh, Dina; Bar-Zvi, Dudy

doi:10.1101/379859

Cited by 3 publications

(3 citation statements)

References 57 publications

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“…PUB22 and PUB23 mediate the degradation of the ABA receptor PYL9 (Zhao et al, 2017). PUB46 overexpression increases plant tolerance to dehydration, pointing to PUB46 as a positive regulator of drought responses (Adler et al, 2018), whereas PUB18 and PUB19 are negative regulators of ABAmediated drought responses (Seo et al, 2012(Seo et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis U‐box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor‐like protein kinases LRR1 and KIN7

Chen

Wang

Rehman

et al. 2021

JIPB

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Both plant receptor‐like protein kinases (RLKs) and ubiquitin‐mediated proteolysis play crucial roles in plant responses to drought stress. However, the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly understood. In this study, we showed that Arabidopsis PLANT U‐BOX PROTEIN 11 (PUB11), an E3 ubiquitin ligase, negatively regulates abscisic acid (ABA)‐mediated drought responses. PUB11 interacts with and ubiquitinates two receptor‐like protein kinases, LEUCINE RICH REPEAT PROTEIN 1 (LRR1) and KINASE 7 (KIN7), and mediates their degradation during plant responses to drought stress in vitro and in vivo. pub11 mutants were more tolerant, whereas lrr1 and kin7 mutants were more sensitive, to drought stress than the wild type. Genetic analyses show that the pub11 lrr1 kin7 triple mutant exhibited similar drought sensitivity as the lrr1 kin7 double mutant, placing PUB11 upstream of the two RLKs. Abscisic acid and drought treatment promoted the accumulation of PUB11, which likely accelerates LRR1 and KIN7 degradation. Together, our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.

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Section: Introductionmentioning

confidence: 99%

Arabidopsis U‐box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor‐like protein kinases LRR1 and KIN7

Chen

Wang

Rehman

et al. 2021

JIPB

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“…According to our results, Soltu01G002820, located in an identical cluster to AtPUB22 and AtPUB23, showed up-regulated expression upon salt and drought stresses and down-regulated under ABA treatment, and up-regulated in DT cultivar Q at S3 stage, indicating the possibly similar functions of Soltu01G002820 to AtPUB22 and AtPUB23 under abiotic stress. Adler et al [28,44] found that AtPUB46 and AtPUB48's single homozygous mutants exhibited hypersensitivity to drought stress, and overexpress of AtPUB46 enhance tolerance to drought and oxidative stress in transgenic Arabidopsis. Soltu09G016850 was clustered with AtPUB46 and AtPUB48, which was up-regulated under salt and drought stress.…”

Section: Expression Pro Le Analysis Of Stpub Genesmentioning

confidence: 99%

Genome-wide Identification and Analysis of the U-box E3 ligases Gene Family in Potato (Solanum tuberosum L.)

Liu

Wang

et al. 2023

Preprint

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Background: Plant U-box (PUB) E3 ubiquitin ligases have vital effects on various biological processes, however, their functions within potato (Solanum tuberosum) remain unclear. Results: This work identified altogether 74 PUBs in potato (StPUBs) and examined their gene structures, chromosomal distributions as well as conserved motifs. There were seventy-four StPUB genes on 10 chromosomes with diverse densities. As revealed by phylogenetic analysis on PUBs within potato, Arabidopsis, together with tomato (Solanum lycopersicum), they were clustered into eight subclasses (C1- C8). Additionally, according to synteny analysis, there were 40 orthologous StPUB genes to Arabidopsis whereas 58 to tomato. In addition, RNA-seq data downloaded from PGSC were utilized to reveal StPUBs’ abiotic stress responses and tissue-specific expression in the doubled-monoploid potato (DM). Furthermore, we performed RNA-seq on the ‘Atlantic’ (A, drought-sensitive cultivar) and the ‘Qingshu NO.9’ (Q, drought-tolerant cultivar) in early flowering, full-blooming, along with flower-falling stages to detect genes that might be involved in response to drought stress. Finally, quantitative real-time PCR (qPCR) was carried out for analyzing three candidate genes for their expression levels within 100 mM NaCl- and 10% PEG 6000 (w/v)-treated potato plantlets for a 24-h period. Conclusion: In this study, three candidate genes related to drought stress tolerance in potato were excavated and these results should provide the valuable information for abiotic stress resistance research of StPUB gene family in potato.

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“…We have studied the paralogous Arabidopsis PUB46, PUB47 and PUB48 genes 29,30 , particularly PUB46 and PUB48 as they are more highly expressed and their mutants give a strong hypersensitivity to water stress.…”

Section: Introductionmentioning

confidence: 99%

The Activity of the Stress Modulated Arabidopsis Ubiquitin Ligases PUB46 and PUB48 is Partially Redundant

Zizelski-Valenci

Raveh

Bar-Zvi

2021

Preprint

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The Arabidopsis ubiquitin ligases PUB46, PUB47 and PUB48 are encoded by paralogus genes; pub46 and pub48 mutants display increased drought sensitivity compared to wild type (WT). Although the phenotype displayed in the single gene mutants, suggest that each has specific biological activity, PUB46 and PUB48 activity may be also redundant. To test functional redundancy between two gene products requires a double mutant. Unfortunately, the close proximity of the PUB46 and PUB48 gene loci precludes obtaining a double mutant by crossing the available single mutants. We thus applied microRNA technology to reduce the activity of all three gene products of the PUB46-48 subfamily by constructing an artificial microRNA (aMIR) targeted to this subfamily. Expressing aMIR46-48 in WT plants resulted in increased drought-sensitivity, a phenotype resembling that of the single pub46 and pub48 mutants, and enhanced sensitivity to methyl viologen, similar to that observed for the pub46 mutant. Furthermore, the WT plants expressing aMIR46-48 also revealed reduced inhibition by ABA at seed germination, a phenotype not evident in the single mutants. Expressing aMIR46-48 in pub46 and pub48 mutants further enhanced the drought sensitivity of each parental single mutant and of WT expressing aMIR46-48. Thus, whereas the gene-specific activity of the PUB46 and PUB48 E3s is partially redundant in that absence of either E3 leads to drought sensitivity, our ability to eliminate the activity of both PUB46 and PUB48 in the same plant reveals additional gene specific facets of their activity in the reaction to abiotic stress.

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Overexpression of Arabidopsis ubiquitin ligase AtPUB46 enhances tolerance to drought and oxidative stress

Cited by 3 publications

References 57 publications

Arabidopsis U‐box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor‐like protein kinases LRR1 and KIN7

Arabidopsis U‐box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor‐like protein kinases LRR1 and KIN7

Genome-wide Identification and Analysis of the U-box E3 ligases Gene Family in Potato (Solanum tuberosum L.)

The Activity of the Stress Modulated Arabidopsis Ubiquitin Ligases PUB46 and PUB48 is Partially Redundant

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