Overexpression of ATP-binding cassette transporters ABCG10, ABCH3 and ABCH4 in Aphis craccivora (Koch) facilitates its tolerance to imidacloprid

Yang, Yuanxue; Duan, Ailing; Zhang, Cong; Zhang, Yun; Wang, Ai‐Yu; Xue, Changxi; Wang, Hongyan; Zhao, Ming; Zhang, Jianhua

doi:10.1016/j.pestbp.2022.105170

Cited by 13 publications

(4 citation statements)

References 38 publications

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“…Overexpression of the SlABCC2 gene in Sf9 cells also increased the toxicity of Cry1Ca toxin to cells [33]. The overexpression of ABCG10, ABCH3, and ABCH4 in Aphis craccivora promoted the tolerance to imidacloprid [61]. In addition, downregulation of the PxABCB1 gene expression significantly reduced the susceptibility of P. xylostella larvae to the Cry1Ac toxin [64].…”

Section: Discussionmentioning

confidence: 97%

“…At present, the two commonly accepted models are sequential binding model [7,52,53] and the signal transduction model [7,8,54], among which the binding of Bt toxins to the receptor in insect midgut is undoubtedly considered to be the key to its action. Up to now, several insect midgut proteins, such as cadherin [8,20,55], aminopeptidase N [18,56,57], alkaline phosphatase [27,58] and ABC transporter [29,32,[59][60][61], have been reported to act as receptors for Bt toxins in the process of Bt action. The polycalin protein involved in this study has been shown to bind to Cry1Ac [43,51] and Cry2Aa [44] toxins in vitro in previous studies, which indicates that it may also act as a receptor protein similar to other known receptors.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout

Xiong

et al. 2023

Toxins

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Many insects, including the Plutella xylostella (L.), have developed varying degrees of resistance to many insecticides, including Bacillus thuringiensis (Bt) toxins, the bioinsecticides derived from Bt. The polycalin protein is one of the potential receptors for Bt toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of P. xylostella, but whether polycalin is associated with the resistance of Bt toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the Pxpolycalin gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of Pxpolycalin showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the Pxpolycalin gene and its transcript level were not linked to Cry1Ac resistance, whereas both the PxABCC2 gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the Pxpolycalin gene in a short term. Furthermore, the knockout of polycalin and ATP-binding cassette transporter subfamily C2 (ABCC2) genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to Bt toxins.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout

Xiong

et al. 2023

Toxins

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“…The two main mechanisms which mediate IMI tolerance are (i) target site mutations of nicotinic acetylcholine receptor (nAchR) subunits which reduce their binding affinity for IMI and (ii) enhanced metabolism of IMI through the over-expression of detoxifying enzymes such as P450 monooxygenases, ATP-binding cassette transporters and gluthatione-S-transferase, among others [29][30][31]. Although target site mutations can produce up to a 250-fold increase in resistance to IMI, as observed in the brown planthopper, N. lugens, enhanced resistance of this magnitude is generally not found in wild populations as the fitness cost of such a mutation (Y151S) is too high [32][33][34].…”

Section: (I) Mechanisms For Imi Tolerancementioning

confidence: 99%

Comparability of comparative toxicity: insect sensitivity to imidacloprid reveals huge variations across species but also within species

Nagloo,

Rigosi,

Herbertsson

et al. 2024

Proc. R. Soc. B.

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Pesticides have been identified as major drivers of insect biodiversity loss. Thus, the study of their effects on non-pest insect species has attracted a lot of attention in recent decades. In general toxicology, the ‘gold standard’ to assess the toxicity of a substance is to measure mass-specific LD 50 (i.e. median lethal dose per unit body mass). In entomology, reviews attempting to compare these data across all available studies are lacking. To fill this gap in knowledge, we performed a systematic review of the lethality of imidacloprid for adult insects. Imidacloprid is possibly the most extensively studied insecticide in recent times, yet we found that little is comparable across studies, owing to both methodological divergence and missing estimates of body mass. By accounting for body mass whenever possible, we show how imidacloprid sensitivity spans across an apparent range of approximately six orders of magnitude across insect species. Very high variability within species can also be observed owing to differences in exposure methods and observation time. We suggest that a more comparable and comprehensive approach has both biological and economic relevance. Ultimately, this would help to identify differences that could direct research towards preventing non-target species from being negatively affected.

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“…Pea aphid ( Acyrthosiphon pisum ) feeds on legume species across the globe, including forage crops like pea, clover, alfalfa, and broad beans [ 2 ]. Aphis craccivora (Koch) feeds on legume plants, and carries bean leaf roll virus and broad bean necrotic yellows virus [ 3 ]. Soybean aphid ( Aphis glycines ), sucks liquid from leaves and stems, causing direct damage to soybean plants [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Comparative Analysis of the Cytochrome P450 Monooxygenase Family in 19 Aphid Species and Their Expression Analysis in 4 Cereal Crop Aphids

Wang,

Hao,

Wang

et al. 2024

IJMS

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Cytochrome P450 monooxygenases (CYP450s) play a variety of physiological roles, including pesticide resistance, plant allelochemical detoxification, and hormone metabolism catalysis. However, limited information is available on the classification and expression profiles of the CYP450 gene family in aphid species. This is the first study to identify the cytochrome P450 gene family in 19 aphid species at the whole genome level. A total of 1100 CYP450 genes were identified in 19 aphid species. Three hundred CYP450 genes belonged to six cereal crop aphid species, which were further classified into four subfamilies according to the phylogenetic relationship. The conserved motifs, exon-intron structures, and genomic organization of the same subfamilies were similar. Predictions of subcellular localization revealed that the endoplasmic reticulum harbored the majority of CYP450 proteins. In Sitobion avenae and Rhopalosiphum maidis, the increase in the CYP450 gene was primarily caused by segmental duplication events. However, only tandem duplication occurred in the CYP450 gene family of Diuraphis noxia, Rhopalosiphum padi, Schizaphis graminum, and Sitobion miscanthi. Synteny analysis found three continuous colinear CYP450 gene pairs among six cereal crop aphid species. Furthermore, we obtained the expression profiles of four cereal crop aphids, including R. padi, D. noxia, S. graminum, and S. avenae. Differential expression analysis provided growth stage specificity genes, tissue specificity genes, organ specificity genes and some detoxification metabolic genes among these four cereal crop aphids. Meanwhile, their expression patterns were showed. The related functions and pathways of CYP450s were revealed by GO and KEGG enrichment analysis. Above all, we picked the differentially expressed CYP450 genes from all of the differentially expressed genes (DEGs). These differentially expressed CYP450 genes provided some new potential candidates for aphid control and management. This work establishes the foundation for further investigations into the regulatory functions of the CYP450 gene family in aphid species and beyond.

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Overexpression of ATP-binding cassette transporters ABCG10, ABCH3 and ABCH4 in Aphis craccivora (Koch) facilitates its tolerance to imidacloprid

Cited by 13 publications

References 38 publications

Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout

Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout

Comparability of comparative toxicity: insect sensitivity to imidacloprid reveals huge variations across species but also within species

Genome-Wide Comparative Analysis of the Cytochrome P450 Monooxygenase Family in 19 Aphid Species and Their Expression Analysis in 4 Cereal Crop Aphids

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