Overexpression of banana GDP-L-galactose phosphorylase (GGP) modulates the biosynthesis of ascorbic acid in Arabidopsis thaliana

Chaturvedi, Siddhant; Thakur, Neha; Khan, Shahirina; Sardar, Mithilesh Kumar; Jangra, Alka; Tiwari, Siddharth

doi:10.1016/j.ijbiomac.2023.124124

Cited by 13 publications

(4 citation statements)

References 51 publications

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“…Meanwhile, the expression of CaGGPs genes gradually decreases as the capsicum s ripened. Similarly, previous study showed that most of the 12 banana varieties contained more AsA in the unripe stage than in the ripe stage 14 .…”

Section: Discussionsupporting

confidence: 59%

Characterization of the GGPgene family inpotato (Solanum tuberosum L.) and Capsicum (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses

Ding,

Yao,

Yao

et al. 2024

Preprint

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Potato (Solanum tuberosum L.) and capsicum (Capsicum annuum L.) are members of the Solanaceae family, and they are widely grown and used worldwide. However, there are significant differences between these two crops in terms of morphology, growing conditions and resistance to stress. But they share some similarities at a genetic level. GDP-L-galactose phosphorylase (GGP) is a key rate-limiting enzyme in plant ascorbic acid synthesis, which plays an important role in plant growth and development as well as stress response. However, the presence of GGP and its function in potato and capsicum are not known. In this study, we first identified two GGP genes in the potato genome using a genome-wide search approach. Similarly, two GGP genes were identified in the capsicum genome. We then analyzed their physicochemical properties, conserved domains, protein structures and phylogenetic relationships. Phylogenetic tree analysis revealed that members of the potato and capsicum GGP gene families are related to cabbage(Brassica campestris), Arabidopsis (Arabidopsis thaliana L.), apple (Malus domestica cv. Gala) and tomato (Solanum lycopersicum L.), with tomato being the most closely related. The promoter sequences mainly contain homeopathic elements such as light-responsive, hormone-responsive and stress-responsive, with light-responsive elements being the most abundant. By analyzing the structure of the genes, it was found that there is no transmembrane structure or signal peptide in the GGP gene family of potatoes and capsicums, and that all of its members are hydrophilic proteins. The expression profiles of the different tissues showed that the potato GGPwas more highly expressed in the leaves and the capsicum GGP was more highly expressed in the fruits. qRT-PCR analysis showed that the GGPgene was responsive to both ABA and MeJA in both the potato and the capsicum. In addition, the GGP genes in both the potato and the capsicum showed different responses to darkness stress, cold stress, salt stress and drought stress. Based on our experimental findings, it is evident that StGGPsprimarily respond to dark stress, while CaGGPs primarily respond to NaCl stress. This study provides a basis for a better understanding of the role of GGP-homologous genes from potato and capsicum in the response of plants to abiotic stress, and lays the foundation for further research into the role of GGPs in plants.

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Section: Discussionsupporting

confidence: 59%

Characterization of the GGPgene family inpotato (Solanum tuberosum L.) and Capsicum (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses

Ding,

Yao,

Yao

et al. 2024

Preprint

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show abstract

“…In addition, the expression of tomato GGP was low in fruits, and the expression tended to decrease with fruit ripening 24 . Similarly, previous study showed that most of the 12 banana varieties contained more AsA in the unripe stage than in the ripe stage 16 .…”

Section: Discussionsupporting

confidence: 59%

“…It has been previously shown that AsA levels in tomato were mainly controlled by post-transcriptional repression of GGP, and down-regulation of GGP resulted in a nearly 1.5-fold reduction in leaf AsA content, highlighting the dominant role of GGP in regulating AsA levels in tomato 15 . The banana ( Musa acuminata ) genome database identified five GGPs 16 . Both rice and maize have a single copy of the GGP gene in their genomes, which is located on the Os11 and Zm6 chromosomes, respectively 9 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of the GGP gene family in potato (Solanum tuberosum L.) and Pepper (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses

Ding,

Yao,

Yao

et al. 2024

Sci Rep

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GDP-l-galactose phosphorylase (GGP) is a key rate-limiting enzyme in plant ascorbic acid synthesis, which plays an important role in plant growth and development as well as stress response. However, the presence of GGP and its function in potato and pepper are not known. In this study, we first identified two GGP genes in each potato and pepper genomes using a genome-wide search approach. We then analyzed their physicochemical properties, conserved domains, protein structures and phylogenetic relationships. Phylogenetic tree analysis revealed that members of the potato and pepper GGP gene families are related to eggplant (Solanum melongena L.), Arabidopsis (Arabidopsis thaliana L.), tobacco (Nicotiana tabacum L.) and tomato (Solanum lycopersicum L.), with tomato being the most closely related. The promoter sequences mainly contain homeopathic elements such as light-responsive, hormone-responsive and stress-responsive, with light-responsive elements being the most abundant. By analyzing the structure of the genes, it was found that there is no transmembrane structure or signal peptide in the GGP gene family of potatoes and peppers, and that all of its members are hydrophilic proteins. The expression profiles of different tissues show that StGGP1 has the highest expression levels in leaves, StGGP2 has the highest expression levels in stamens, and CaGGPs have the highest expression levels in the early stages of fruit development (Dev1). It was found that StGGPs and CaGGPs genes showed different response to phytohormones and abiotic stresses. Abscisic acid (ABA) treatment induced the most significant change in the expression of StGGPs, while the expression of CaGGPs showed the most pronounced change under methyl jasmonate (MeJA) treatment. StGGPs responded mainly to dark treatment, whereas CaGGPs responded mainly to NaCl stress. These results provide an important basis for a detailed study about the functions of GGP homologous genes in potato and pepper in response to abiotic stresses.

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“…H 2 O 2 has strong oxidability and toxicity when its content too high 30 . AsA, also known as vitamin C, 31 has strong reducibility and can remove peroxidation substances in organisms. AsA is a highly effective antioxidant.…”

Section: Resultsmentioning

confidence: 99%

Stereoselectivity of paclobutrazol enantiomers to oxidative stress in wheat

Yang,

Li,

Liang

et al. 2024

Chirality

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Chiral pesticides have the special chiral structures, so enantioselective biological effects are usually observed in living organisms. Current study used paclobutrazol as a case study and explored the enantioselective degradation and oxidative stress effect on wheat. The results demonstrated that the degradation of R‐paclobutrazol was faster than S‐paclobutrazol significantly and improved the content of MDA and O2− in wheat plants, which proved that the R‐paclobutrazol induced oxidative damage in wheat, showing selective biological effects, and S‐paclobutrazol was friendly to wheat. This study provided a theoretical basis for the selective activity of chiral pesticides and the development of chiral pesticide monomers.

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Overexpression of banana GDP-L-galactose phosphorylase (GGP) modulates the biosynthesis of ascorbic acid in Arabidopsis thaliana

Cited by 13 publications

References 51 publications

Characterization of the GGPgene family inpotato (Solanum tuberosum L.) and Capsicum (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses

Characterization of the GGPgene family inpotato (Solanum tuberosum L.) and Capsicum (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses

Characterization of the GGP gene family in potato (Solanum tuberosum L.) and Pepper (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses

Stereoselectivity of paclobutrazol enantiomers to oxidative stress in wheat

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