Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus

Wang, Yanmei; Jin, Shurong; Xu, Yi; Li, Shuai; Zhang, Shuangjuan; Yuan, Zheng; Li, Jiana; Ni, Yu

doi:10.1016/j.cj.2019.04.006

Cited by 50 publications

(43 citation statements)

References 36 publications

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“…For example, overexpression of AhKCS1 from a drought tolerant groundnut reduced the cuticle permeability and enhanced the drought tolerance in a susceptible genotype by increasing cuticular wax load in leaves ( Lokesh et al, 2019 ). Overexpression of BnKCS1-1 and BnKCS1-2 in Brassica napus also increased drought tolerance in transgenic plants by promoting cuticular wax production ( Wang et al, 2020 ). As reviewed by Singh et al (2018) , a number of other genes related to wax biosynthesis also have the function to enhance the resistance to abiotic stress by altering wax composition, such as WIN1/SHN1 , DWA1 , MYB96 , CER1 , CER3/WAX2 , FAR family genes and so on.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis ( Zhang et al, 2019b ). Overexpression of BnKCS1-1 , BnKCS1-2 , and BnCER1-2 in Brassica napus increased the wax crystal density on the leaf surface, and the water loss rate decreased together with increased drought tolerance, which was enhanced in transgenic plants ( Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Ectopic Expression of CsKCS6 From Navel Orange Promotes the Production of Very-Long-Chain Fatty Acids (VLCFAs) and Increases the Abiotic Stress Tolerance of Arabidopsis thaliana

Guo

Yang

et al. 2020

Front. Plant Sci.

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Cuticular wax is closely related to plant resistance to abiotic stress. 3-Ketoacyl-CoA synthase (KCS) catalyzes the biosynthesis of very-long-chain fatty acid (VLCFA) wax precursors. In this study, a novel KCS family gene was isolated from Newhall navel orange and subsequently named CsKCS6 . The CsKCS6 protein has two main domains that belong to the thiolase-like superfamily, the FAE1-CUT1-RppA and ACP_syn_III_C domains, which exist at amino acid positions 80–368 and 384–466, respectively. CsKCS6 was expressed in all tissues, with the highest expression detected in the stigma; in addition, the transcription of CsKCS6 was changed in response to drought stress, salt stress and abscisic acid (ABA) treatment. Heterologous expression of CsKCS6 in Arabidopsis significantly increased the amount of VLCFAs in the cuticular wax on the stems and leaves, but there were no significant changes in total wax content. Compared with that of the wild-type (WT) plants, the leaf permeability of the transgenic plants was lower. Further research showed that, compared with the WT plants, the transgenic lines experienced less water loss and ion leakage after dehydration stress, displayed increased survival under drought stress treatment and presented significantly longer root lengths and survival under salt stress treatment. Our results indicate that CsKCS6 not only plays an important role in the synthesis of fatty acid precursors involved in wax synthesis but also enhances the tolerance of transgenic Arabidopsis plants to abiotic stress. Thus, the identification of CsKSC6 could help to increase the abiotic stress tolerance of Citrus in future breeding programs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ectopic Expression of CsKCS6 From Navel Orange Promotes the Production of Very-Long-Chain Fatty Acids (VLCFAs) and Increases the Abiotic Stress Tolerance of Arabidopsis thaliana

Guo

Yang

et al. 2020

Front. Plant Sci.

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“…The cuticular wax composition of leaves from all accessions was determined as described by Wang et al [8] with some modifications. Three leaves (third leaf from the top) were sampled from three plants in each replicate for each accession.…”

Section: Cuticular Wax Analysismentioning

confidence: 99%

“…The aerial parts of plants are covered with cuticular wax, a mixture of hydrophobic compounds. Both increased cuticular wax deposition and improved tolerance to water deficiency stress were observed in some transgenic crops with the overexpression of wax-associated genes [5][6][7][8], suggesting that this hydrophobic layer could protect plants from abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

“…The product of the KCS1 encodes a condensing enzyme KCS1 (3-ketoacyl-CoA synthase 1) which is involved in the critical fatty acid elongation process in wax precursor biosynthesis [13]. Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 in B. napus promoted cuticular wax production and increases drought tolerance [14]. Overexpression of BnLAS, a member of the GRAS family of putative transcriptional regulators, resulted in inhibition of growth, delays in leaf senescence and flowering time, and more epidermal wax deposition on transgenic leaves of Arabidopsis [15].…”

Section: Introductionmentioning

confidence: 99%

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A combination of genome-wide association study and transcriptome analysis in leaf epidermis identifies candidate genes involved in cuticular wax biosynthesis in Brassica napus

et al. 2020

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Background Brassica napus L. is one of the most important oil crops in the world. However, climate-change-induced environmental stresses negatively impact on its yield and quality. Cuticular waxes are known to protect plants from various abiotic/biotic stresses. Dissecting the genetic and biochemical basis underlying cuticular waxes is important to breed cultivars with improved stress tolerance. Results Here a genome-wide association study (GWAS) of 192 B. napus cultivars and inbred lines was used to identify single-nucleotide polymorphisms (SNPs) associated with leaf waxes. A total of 202 SNPs was found to be significantly associated with 31 wax traits including total wax coverage and the amounts of wax classes and wax compounds. Next, epidermal peels from leaves of both high-wax load (HW) and low-wax load (LW) lines were isolated and used to analyze transcript profiles of all GWAS-identified genes. Consequently, 147 SNPs were revealed to have differential expressions between HW and LW lines, among which 344 SNP corresponding genes exhibited up-regulated while 448 exhibited down-regulated expressions in LW when compared to those in HW. According to the gene annotation information, some differentially expressed genes were classified into plant acyl lipid metabolism, including fatty acid-related pathways, wax and cutin biosynthesis pathway and wax secretion. Some genes involved in cell wall formation and stress responses have also been identified. Conclusions Combination of GWAS with transcriptomic analysis revealed a number of directly or indirectly wax-related genes and their associated SNPs. These results could provide clues for further validation of SNPs for marker-assisted breeding and provide new insights into the genetic control of wax biosynthesis and improving stress tolerance of B. napus.

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Developing drought‐smart, ready‐to‐grow future crops

Mubarik²,

et al. 2022

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Breeding crop plants with increased yield potential and improved tolerance to stressful environments is critical for global food security. Drought stress (DS) adversely affects agricultural productivity worldwide and is expected to rise in the coming years. Therefore, it is vital to understand the physiological, biochemical, molecular, and ecological mechanisms associated with DS. This review examines recent advances in plant responses to DS to expand our understanding of DS-associated mechanisms. Suboptimal water sources adversely affect crop growth and yields through physical impairments, physiological disturbances, biochemical modifications, and molecular adjustments. To control the devastating effect of DS in crop plants, it is important to understand its consequences, mechanisms, and the agronomic and genetic basis of DS for sustainable production. In addition to plant responses, we highlight several mitigation options such as omics approaches, transgenics breeding, genome editing, and biochemical to mechanical methods (foliar treatments, seed priming, and conventional agronomic practices). Further, we have

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Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus

Cited by 50 publications

References 36 publications

Ectopic Expression of CsKCS6 From Navel Orange Promotes the Production of Very-Long-Chain Fatty Acids (VLCFAs) and Increases the Abiotic Stress Tolerance of Arabidopsis thaliana

Ectopic Expression of CsKCS6 From Navel Orange Promotes the Production of Very-Long-Chain Fatty Acids (VLCFAs) and Increases the Abiotic Stress Tolerance of Arabidopsis thaliana

A combination of genome-wide association study and transcriptome analysis in leaf epidermis identifies candidate genes involved in cuticular wax biosynthesis in Brassica napus

Developing drought‐smart, ready‐to‐grow future crops

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