Overexpression of BpCUC2 Influences Leaf Shape and Internode Development in Betula pendula

Liu, Chaoyi; Xu, Huirong; Han, Rui; Wang, Shuo; Su, Chen; Chen, Jiying; Bian, Xiuyan; Jiang, Jing

doi:10.3390/ijms20194722

Cited by 11 publications

(9 citation statements)

References 52 publications

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“…Eventually, the explants were placed onto co-cultivation medium (WPM with 0.8 mg/L 6-BA, 0.02 mg/L NAA) under dark for 2 days and then planted on selective medium (WPM with 2.0 mg/L 6-BA, 0.02 mg/L NAA, 5.5mg/L hygromycin or glufosinate ammonium and 200 mg/L cefotaxime). After 30 days, resistant adventitious buds were induced (WPM with 2.0mg/L 6-BA, 0.02 mg/L NAA, 0.5mg/L GA,5.5mg/L hygromycin or glufosinate ammonium and 200 mg/L cefotaxime) and rooted (WPM + 0.4mg/L IBA) ( Liu et al, 2019 ). After raised to 5 cm high in growth chamber, transgenic plants were transferred to the greenhouse.…”

Section: Methodsmentioning

confidence: 99%

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Xing

Zhang

et al. 2021

Front. Plant Sci.

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PP2C protein phosphatase family is one of the largest gene families in the plant genome. Many PP2C family members are involved in the regulation of abiotic stress. We found that BpPP2C1 gene has highly up-regulated in root under salt stress in Betula platyphylla. Thus, transgenic plants of Betula platyphylla with overexpression and knockout of BpPP2C1 gene were generated using a zygote transformation system. Under NaCl stress treatment, we measured the phenotypic traits of transgenic plants, chlorophyll-fluorescence parameters, peroxidase (POD) activity, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content. We found that BpPP2C1 overexpressed lines showed obvious salt tolerance, while BpPP2C1 knocked out plants were sensitive to salt stress. Transcriptome analysis identified significantly amount of differentially expressed genes associated with salt stress in BpPP2C1 transgenic lines, especially genes in abscisic acid signaling pathway, flavonoid biosynthetic pathway, oxidative stress and anion transport. Functional study of BpPP2C1 in Betula platyphylla revealed its role in salt stress.

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Section: Methodsmentioning

confidence: 99%

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Xing

Zhang

et al. 2021

Front. Plant Sci.

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“…Previous studies have suggested that CUC2 plays important roles in adventitious shoot formation ( Daimon, Takabe & Tasaka, 2003 ) and floral organ development ( Aida et al, 1997 ; Baker et al, 2005 ; Kamiuchi et al, 2014 ; Gonzalez-Carranza et al, 2017 ). Moreover, CUC2 plays a role in internode development, with BpCUC2 overexpression leading to internode shortening in Betula pendula ( Liu et al, 2019 ). Taken together, these observations suggest that CUC2 participates in both growth and development in plants.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the LcCUC2-like gene in Arabidopsis thaliana alters the cotyledon morphology and increases rosette leaf number

Wen

Hao

et al. 2022

PeerJ

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Background The unique ‘mandarin jacket’ leaf shape is the most famous trait of Liriodendron chinense and this characteristic gives L. chinense aesthetic and landscaping value. However, the underlying regulatory mechanism of genes involved in the leaf development of L. chinense has remained unclear. Methods Based on transcriptome data of leaves at different developmental stages from L. chinense, we identified differentially expression genes (DEGs) functioning in leaf development. A candidate gene named LcCUC2-like (LcCUC2L) had high similarity in sequence with Arabidopsis thaliana CUC2, and used for further research. We isolated the full-length LcCUC2L gene and its promoter from L. chinense. Subsequently, we analyzed the function of the LcCUC2L gene and its promoter activity via transformation into A. thaliana. Results In this study, we found that the LcCUC2L and AtCUC2 are homologous in sequence but not homologous in function. Unlike the role of AtCUC2 in leaf serration and SAM formation, the LcCUC2L mainly regulates cotyledon development and rosette leaf number. Histochemical β-glucuronidase (GUS) staining revealed that LcCUC2L was expressed in the cotyledons of A. thaliana seedlings, indicating that the LcCUC2L may play a role in cotyledon development. Ectopic expression of LcCUC2L resulted in long, narrow cotyledons without petioles, abnormal lamina epidermis cells and defective vascular tissue in cotyledons, and these results were consistent with the LcCUC2L expression pattern. Further analysis showed that overexpression of LcCUC2L also induced numerous rosette leaves. Also, LcCUC2L and other related genes showed a severe response in L. chinense by introducing exogenous auxin stimulation, partly revealed that LcCUC2L affects the leaf development by regulating the auxin content. Conclusions These results suggest that LcCUC2L may play a critical role in leaf development and morphogenesis in L. chinense, and our findings provide insight into the molecular mechanisms of leaf development in L. chinense.

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“…Plants were cut and grown on 0.2 mg L −1 NAA 0.5 Murashige and Skoog (0.5 MS) medium with 1% sucrose and 0.75% agar (pH 6) (16-h light and 8-h dark photoperiod) when the adventitious buds grew up. At 1-month-old, seedlings in 1 2 MS medium were transplanted into a 45-plug tray (3 cm in diameter by 3 cm in height). The seedling growth substratum was black soil (v/v):perlite:vermiculite (4:2:2) and maintained in a growth room at 24 ± 1 • C with a 16-h light and 8-h dark photoperiod.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…White birch (Betula platyphylla S.) is a revolutionary forest plant in northeastern China [1], which is one of the most valuable, important, and widely distributed tree species in east Asia, mostly in China, Korea, and Japan [2]. Betula platyphylla has a rapid growth rate, excellent material properties, high artificial planting survival rate, and a wide range of applications in the manufacture, pharmacy, and landscaping of furniture [1,[3][4][5]. In recent years, several research contributions of B. platyphylla under abiotic stresses can be found, such as drought stress [6,7], salt stress [8], ozone stress [9], heat stress [10], and cold stress [11,12], yet the knowledge is still inadequate and needs to be further explored.…”

Section: Introductionmentioning

confidence: 99%

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Cold Acclimation Affects Physiological and Biochemical Characteristics of Betula platyphylla S. under Freezing Stress

Ritonga

Yan

Chen

et al. 2021

Forests

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Cold and freezing stress is one of the most harmful environmental stresses, especially in temperate and subtropical areas, that adversely affects plant growth, development, and yield production. Betula platyphylla Sukaczev, also known as white birch, is one of the most valuable, important, and widely distributed tree species in East Asia. This study explored the effects of cold acclimation (CA) in reducing the destructive effect of freezing stress in B. platyphylla seedlings. We measured the physiological and biochemical characteristics of B. platyphylla seedlings, such as chlorophyll content, electrolyte leakage (EL), malondialdehyde (MDA), antioxidant enzymes (such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), and proline content before and after freezing stress to observe the contribution of CA in reducing the detrimental effects of freezing stress. The results showed that CA increased physiological and biochemical characteristics of B. platyphylla seedlings before and after freezing stress, except for chlorophyll content. Antioxidant enzymes were significantly positively correlated with proline, MDA, and EL content, and negatively correlated with chlorophyll content. Moreover, histochemical detection (H2O2 and O2−) and cell death were revealed to be induced by cold stress in B. platyphylla seedlings. Furthermore, it was revealed that increased time and decreased temperature of the CA process significantly influenced the physiological and biochemical parameters. Overall, the CA process significantly reduced the detrimental effects of freezing stress compared to the control treatment in B. platyphylla seedlings. Taken together, these findings provide beneficial information toward understanding the mechanism of CA and freezing stress in B. platyphylla. Furthermore, the substantial activity of physiological and biochemical results could be used as selection criteria for screening time and temperature points of cold/freezing stress in further omics analyses. In addition, the combination of current study results, further omics analyses, and genetic engineering techniques directly contribute to sustainable forest management systems, tree plantations, and conservation of tree species, especially non-cold/non-freezing tolerant tree species.

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Overexpression of BpCUC2 Influences Leaf Shape and Internode Development in Betula pendula

Cited by 11 publications

References 52 publications

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Overexpression of the LcCUC2-like gene in Arabidopsis thaliana alters the cotyledon morphology and increases rosette leaf number

Cold Acclimation Affects Physiological and Biochemical Characteristics of Betula platyphylla S. under Freezing Stress

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