Overexpression of BrAFP1 gene from winter rapeseed (Brassica rapa) confers cold tolerance in Arabidopsis

Dong, Xiaoyun; Zigang, Liu; Mi, Wenbo; Xu, Chunmei; Xu, Minxia; Zhou, Ya; Zhen, Guoqiang; Cao, Xiaodong; Fang, Xinlin; Mi, Chao

doi:10.1016/j.plaphy.2020.07.011

Cited by 11 publications

(4 citation statements)

References 30 publications

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“…For overwinter plants or plants living in cold regions, the exposure of plants to freezing stress can lead to the formation of ice crystals in the extracellular space of plant cells, and large ice crystals can physically damage the plasma membrane [ 40 ]. Interestingly, as one of the osmotic cryoprotectants, during the freezing process, Pro could penetrate through the cell membrane quickly, bind intracellular water hydrogen bonds, inhibit the formation of ice crystals at freezing temperatures, and protect protein integrity, and cell membranes [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes

Zhao

Niu

et al. 2022

Plants

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Maize is a cold-sensitive crop, and it exhibits severe retardation of growth and development when exposed to cold snaps during and right after seedling emergence. Although different agronomic, physiological, and molecular approaches have been tried to overcome the problems related to cold stress in recent years, the mechanisms causing cold resistance in maize are still unclear. Screening and breeding of varieties for cold resistance may be a sustainable option to boost maize production under low-temperature environments. Herein, seedlings of 39 different maize genotypes were treated under both 10 °C low temperature and 22 °C normal temperature conditions for 7 days, to assess the changes in seven growth parameters, two membrane characteristics, two reactive oxygen species (ROS) levels, and four antioxidant enzymes activities. The changes in ten photosynthetic performances, one osmotic substance accumulation, and three polyamines (PAs) metabolisms were also measured. Results indicated that significant differences among genotypes, temperature treatments, and their interactions were found in 29 studied traits, and cold–stressed seedlings were capable to enhance their cold resistance by maintaining high levels of membrane stability index (66.07%); antioxidant enzymes activities including the activity of superoxide dismutase (2.44 Unit g−1 protein), peroxidase (1.65 Unit g−1 protein), catalase (0.65 μM min−1 g−1 protein), and ascorbate peroxidase (5.45 μM min−1 g−1 protein); chlorophyll (Chl) content, i.e., Chl a (0.36 mg g−1 FW) and Chl b (0.40 mg g−1 FW); photosynthetic capacity such as net photosynthetic rate (5.52 μM m−2 s−1) and ribulose 1,5–biphosphate carboxylase activity (6.57 M m−2 s−1); PAs concentration, mainly putrescine (274.89 nM g−1 FW), spermidine (52.69 nM g−1 FW), and spermine (45.81 nM g−1 FW), particularly under extended cold stress. Importantly, 16 traits can be good indicators for screening of cold–resistant genotypes of maize. Gene expression analysis showed that GRMZM2G059991, GRMZM2G089982, GRMZM2G088212, GRMZM2G396553, GRMZM2G120578, and GRMZM2G396856 involved in antioxidant enzymes activity and PAs metabolism, and these genes may be used for genetic modification to improve maize cold resistance. Moreover, seven strong cold–resistant genotypes were identified, and they can be used as parents in maize breeding programs to develop new varieties.

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Section: Discussionmentioning

confidence: 99%

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes

Zhao

Niu

et al. 2022

Plants

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“…It is also possible to take advantage of advances in biotechnological advances to clone AFPs. AFPs were successfully cloned from Solanum dulcamara ( Huang and Duman, 2002 ), Dendroctonus armandi ( Fu et al., 2022 ), Brassica rapa ( Liu et al., 2019 ; Dong et al., 2020 ), Zoarces americanus (type III, Hobbs et al., 2020 ), Gadus macrocephalus (type IV, Mao et al., 2018 ), Hypogastrura harveyi ( Chen et al., 2022 ), Lycodichthys dearborni (type III, Huang et al., 2019 ), Cottoidea (type I, Yamazaki et al., 2019 ), Apis cerana cerana ( Xu et al., 2018 ), Ammopiptanthus nanus ( Zhang et al., 2021 ), Larimichthys Crocea ( Qian, 2021 ), Glaciozyma antarctica ( Firdaus-raih et al., 2018 ), Chloromonas sp. KNF0032 (type II, Cho et al., 2019 ), Brachypodium distachyon ( Bredow et al., 2018 ).…”

Section: Antifreeze Proteins As Cryoprotective Agents For Foodsmentioning

confidence: 99%

Effect of antifreeze proteins on the freeze-thaw cycle of foods: fundamentals, mechanisms of action, current challenges and recommendations for future work

Tirado-Kulieva¹,

Miranda-Zamora²,

Hernández-Martínez

et al. 2022

Heliyon

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“…Griffith et al ., 1992 found the existence of plant‐derived AFP in winter rye leaves, which is the first time to find plant endogenous AFP (Provesi et al ., 2019). Later, more and more plant‐derived AFPs were found or isolated, including carrots (Wang et al ., 2020), Ligustrum lucidum (Cai et al ., 2011), Hippophae rhamnoides (Gupta & Deswal, 2012), winter rapeseed (Dong et al ., 2020), quinoa (Cen et al ., 2022), fruit trees (Hillmann et al ., 2020), etc.…”

Section: Introductionmentioning

confidence: 99%

Recent advances, challenges and functional applications of antifreeze protein in food industry

Xiao,

Hu,

Zhu

et al. 2023

Int J of Food Sci Tech

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SummaryFreezing is a common food preservation method that can extend the shelf life of food, but the ice crystals and recrystallisation during freezing, storage and transportation cause significant damage to frozen food. Antifreeze proteins are a class of proteins that widely exist in organisms living in cold conditions, such as fish, insects, plants and microorganisms in Antarctica and other cold regions. Antifreeze proteins can reduce the freezing point of water, inhibit recrystallisation effects, modify ice crystal morphology and effectively suppress the quality deterioration caused by ice crystals during food freezing. This review comprehensively introduces the source, antifreeze mechanism, application in food, influencing factors and regulating methods of antifreeze activity of antifreeze proteins, as well as the limitations of antifreeze proteins. It also looks forward to the future application of antifreeze proteins in food.

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Overexpression of BrAFP1 gene from winter rapeseed (Brassica rapa) confers cold tolerance in Arabidopsis

Cited by 11 publications

References 30 publications

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes

Effect of antifreeze proteins on the freeze-thaw cycle of foods: fundamentals, mechanisms of action, current challenges and recommendations for future work

Recent advances, challenges and functional applications of antifreeze protein in food industry

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