Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide

Dabral, Neha; Jain-Gupta, Neeta; Seleem, Mohamed N.; Sriranganathan, Nammalwar; Vemulapalli, Ramesh

doi:10.3389/fcimb.2015.00054

Cited by 9 publications

(6 citation statements)

References 45 publications

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“…In turn, EM strains uniquely evidenced the Listeria Adhesion Protein, that interacts with the Hsp60 host-cell receptor to promote bacterial adhesion in Listeria ssp. (Jagadeesan et al, 2010 ); the fic/DOC family protein, a toxin that mediates post-translational modifications of host cell proteins, thus specifically interfering with signaling, cytoskeletal processes, or translation (Roy and Cherfils, 2015 ); and a putative glycosyltransferase, which is essential for O-polysaccharide synthesis (Dabral et al, 2015 ). These genogroup-specific virulence factors could grant special traits.…”

Section: Discussionmentioning

confidence: 99%

Comparative Pan-Genome Analysis of Piscirickettsia salmonis Reveals Genomic Divergences within Genogroups

Nourdin-Galindo

Sánchez

Molina

et al. 2017

Front. Cell. Infect. Microbiol.

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Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects the salmonid industry. Despite efforts to genomically characterize P. salmonis, functional information on the life cycle, pathogenesis mechanisms, diagnosis, treatment, and control of this fish pathogen remain lacking. To address this knowledge gap, the present study conducted an in silico pan-genome analysis of 19 P. salmonis strains from distinct geographic locations and genogroups. Results revealed an expected open pan-genome of 3,463 genes and a core-genome of 1,732 genes. Two marked genogroups were identified, as confirmed by phylogenetic and phylogenomic relationships to the LF-89 and EM-90 reference strains, as well as by assessments of genomic structures. Different structural configurations were found for the six identified copies of the ribosomal operon in the P. salmonis genome, indicating translocation throughout the genetic material. Chromosomal divergences in genomic localization and quantity of genetic cassettes were also found for the Dot/Icm type IVB secretion system. To determine divergences between core-genomes, additional pan-genome descriptions were compiled for the so-termed LF and EM genogroups. Open pan-genomes composed of 2,924 and 2,778 genes and core-genomes composed of 2,170 and 2,228 genes were respectively found for the LF and EM genogroups. The core-genomes were functionally annotated using the Gene Ontology, KEGG, and Virulence Factor databases, revealing the presence of several shared groups of genes related to basic function of intracellular survival and bacterial pathogenesis. Additionally, the specific pan-genomes for the LF and EM genogroups were defined, resulting in the identification of 148 and 273 exclusive proteins, respectively. Notably, specific virulence factors linked to adherence, colonization, invasion factors, and endotoxins were established. The obtained data suggest that these genes could be directly associated with inter-genogroup differences in pathogenesis and host-pathogen interactions, information that could be useful in designing novel strategies for diagnosing and controlling P. salmonis infection.

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Section: Discussionmentioning

confidence: 99%

Comparative Pan-Genome Analysis of Piscirickettsia salmonis Reveals Genomic Divergences within Genogroups

Nourdin-Galindo

Sánchez

Molina

et al. 2017

Front. Cell. Infect. Microbiol.

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“…We discovered 14 such synthetic lethal genes in B. abortus (Fig. B and C) that have been previously reported to play a role in O‐polysaccharide synthesis (Cloeckaert et al ., ; Ugalde et al ., ; Guzman‐Verri et al ., ; Monreal et al ., ; Manterola et al ., ; Cardoso et al ., ; Lamontagne et al ., ; Gonzalez et al ., ; Mancilla et al ., ; Zhang et al ., ; Tian et al ., ; Dabral et al ., ; Salvador‐Bescos et al ., ); mutations in this gene set result in a rough phenotype in Brucella melitensis (Gonzalez et al ., ). It is well established that B. ovis is a stably rough strain that lacks LPS O‐polysaccharide (Gonzalez et al ., ; Tsolis et al ., ; Soler‐Llorens et al ., ; Mancilla, ).…”

Section: Resultsmentioning

confidence: 99%

Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus

Herrou

Willett

Fiebig

et al. 2019

Molecular Microbiology

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Summary Molecular components of the Brucella abortus cell envelope play a major role in its ability to infect, colonize and survive inside mammalian host cells. In this study, we have defined a role for a conserved gene of unknown function in B. abortus envelope stress resistance and infection. Expression of this gene, which we name eipA, is directly activated by the essential cell cycle regulator, CtrA. eipA encodes a soluble periplasmic protein that adopts an unusual eight‐stranded β‐barrel fold. Deletion of eipA attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to treatments that compromise the cell envelope integrity. Transposon disruption of genes required for LPS O‐polysaccharide biosynthesis is synthetically lethal with eipA deletion. This genetic connection between O‐polysaccharide and eipA is corroborated by our discovery that eipA is essential in Brucella ovis, a naturally rough species that harbors mutations in several genes required for O‐polysaccharide production. Conditional depletion of eipA expression in B. ovis results in a cell chaining phenotype, providing evidence that eipA directly or indirectly influences cell division in Brucella. We conclude that EipA is a molecular determinant of Brucella virulence that functions to maintain cell envelope integrity and influences cell division.

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“…Specifically, we sought to identify genes that are dispensable in wild-type cells, but essential in the absence of eipA. We discovered 14 such synthetic lethal genes in B. abortus ( Figure 6B and C) that have been previously reported to play a role in O-polysaccharide synthesis (Gonzalez et al, 2008, Cardoso et al, 2006, Ugalde et al, 2000, Zhang et al, 2013, Tian et al, 2014, Mancilla et al, 2012, Cloeckaert et al, 2000, Dabral et al, 2015, Monreal et al, 2003, Guzman-Verri et al, 2002, Lamontagne et al, 2007, Manterola et al, 2005, Salvador-Bescos et al, 2018; mutations in this gene set result in a rough phenotype in Brucella melitensis (Gonzalez et al, 2008). It is well established that B. ovis is a stably rough strain that lacks LPS Opolysaccharide (Mancilla, 2015, Soler-Llorens et al, 2014, Tsolis et al, 2009, Gonzalez et al, 2008.…”

Section: Eipa Deletion Results In Sensitivity To Cell Envelope Stressorsmentioning

confidence: 99%

Section: Eipa Is Essential In Brucella Ovis and Is Synthetically Lethmentioning

confidence: 99%

Periplasmic protein EipA determines envelope stress resistance and virulence inBrucella abortus

Herrou¹,

Willett²,

Fiebig³

et al. 2018

Preprint

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Molecular components of the Brucella abortus cell envelope, including lipopolysaccharide, play a major role in its ability to infect, colonize and survive inside mammalian host cells. We have defined a role for a conserved gene of unknown function in B. abortus envelope stress resistance and infection. Expression of this gene, which we name eipA, is directly activated by the essential cell cycle regulator, CtrA. eipA encodes a soluble periplasmic protein that adopts an unusual eight-stranded β -barrel fold. Deletion of eipA attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to cell envelope stressors in vitro. Transposon disruption of genes required for LPS O-polysaccharide biosynthesis is synthetically lethal with eipA deletion. This genetic connection between O-polysaccharide and eipA is corroborated by our discovery that eipA is essential in Brucella ovis, a rough species that harbors mutations in several genes required for O-polysaccharide production. Conditional depletion of eipA expression in B. ovis results in a cell chaining phenotype, providing evidence that eipA directly or indirectly influences cell division in Brucella. Our data provide evidence that EipA is a molecular determinant of Brucella virulence that functions to maintain cell envelope integrity and influences cell division.

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Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide

Cited by 9 publications

References 45 publications

Comparative Pan-Genome Analysis of Piscirickettsia salmonis Reveals Genomic Divergences within Genogroups

Comparative Pan-Genome Analysis of Piscirickettsia salmonis Reveals Genomic Divergences within Genogroups

Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus

Periplasmic protein EipA determines envelope stress resistance and virulence inBrucella abortus

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