Overexpression of Camellia sinensis Thaumatin-Like Protein, CsTLP in Potato Confers Enhanced Resistance to Macrophomina phaseolina and Phytophthora infestans Infection

Acharya, Karan; Pal, Awadhesh Kumar; Gulati, Arvind; Kumar, Sanjay; Singh, Anil Kumar; Ahuja, Paramvir Singh

doi:10.1007/s12033-012-9603-y

Cited by 76 publications

(46 citation statements)

References 66 publications

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“…Furthermore, the recovery of several TLP genes only in NAD at early time point and one expressed in both genotypes but strongly up-regulated in NAD confirmed the efficient response of the resistant plant against FOM1.2. TLPs are induced in response to pathogens attack and exhibit antifungal property when over-expressed in transgenic plants, probably by inhibiting hyphal growth, and spore germination (Acharya et al, 2013). The existence of calcium related-proteins in the plant nucleus, including CaM, CaM-binding protein, CDPK, and Ca 2+− CaM-regulated protein phosphatase underline the involvement of calcium in signaling (Lecourieux et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Melon-Fusarium oxysporum f. sp. melonis Race 1.2 Pathosystem in Susceptible and Resistant Plants

Sebastiani¹,

Bagnaresi²,

Sestili³

et al. 2017

Front. Plant Sci.

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Fusarium oxysporum f. sp. melonis Snyd. & Hans race 1.2 (FOM1.2) is the most virulent and yield-limiting pathogen of melon (Cucumis melo L.) worldwide. Current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. RNA-Seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-FOM1.2 interaction in the resistant doubled haploid line NAD and in the susceptible genotype Charentais-T (CHT) at 24 and 48 h post-inoculation (hpi). The transcriptome analysis of the NAD-FOM1.2 interaction identified 2,461 and 821 differentially expressed genes (DEGs) at 24 hpi and at 48 hpi, respectively, while in susceptible combination CHT-FOM1.2, 882 and 2,237 DEGs were recovered at 24 hpi and at 48 hpi, respectively. The overall expression profile suggests a prompt activation of the defense responses in NAD due to its basal defense-related machinery that allows an early pathogen recognition. Gene Ontology (GO) enrichment analyses revealed a total of 57 GO terms shared by both genotypes and consistent with response to fungal infection. GO classes named “chitinase activity,” “cellulase activity,” “defense response, incompatible interaction,” “auxin polar transport” emerged as major factors of resistance to FOM1.2. The data indicated that NAD reacts to FOM1.2 with a fine regulation of Ca2+-mediated signaling pathways, cell wall reorganization, and hormone crosstalk (jasmonate and ethylene, auxin and abscissic acid). Several unannotated transcripts were recovered providing a basis for a further exploration of the melon resistance genes. DEGs belonging to the FOM1.2 genome were also detected in planta as a resource for the identification of potential pathogenicity factors. This work provides a broader view of the dynamic changes of the melon transcriptome triggered by FOM1.2 and highlights that the resistance response of NAD is mainly signaled by jasmonic acid and ethylene pathways mediated by ABA and auxin. The role of candidate plant and fungal responsive genes involved in the resistance is discussed.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Melon-Fusarium oxysporum f. sp. melonis Race 1.2 Pathosystem in Susceptible and Resistant Plants

Sebastiani¹,

Bagnaresi²,

Sestili³

et al. 2017

Front. Plant Sci.

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“…TLP belongs to the PR-5 family based on the amino acid sequence and structural similarities (Edens et al, 1982). Overexpression of TLP enhances tolerance to different fungus (Acharya et al, 2013; Mackintosh et al, 2007; Munis et al, 2010), salt, drought, and oxidative stresses (Munis et al, 2010). The DUF26-like protein is also involved in multiples stress tolerance, such as rice blast fungus (Kim et al, 2004), salt stress (Zhang et al, 2009), and ozone stress (Wrzaczek et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice

Kim

Kang

et al. 2016

The Plant Pathology Journal

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Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named “jasmonic acid inducible pathogenesis-related class 10” (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.

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“…In agreement with our fi nding, Zamani et al (24) have reported that transgenic canola harboring rye tlp gene could inhibit the progress of the S. sclerotiorum fungal growth (24). The same fi ndings have also reported that overexpression of tlp gene demonstrates an inhibitory eff ect on phytopathogenic fungi through inhibition of the spore germination and mycelial growth (11,25,26).…”

Section: Discussionmentioning

confidence: 57%

“…Genetic manipulation of the plants is one of the most effi cient methods for management of the fungal diseases. TLPs as a class of PR-5 family, have been isolated and characterized from diff erent plants such as corn (16), rye (23), Camellia sinensis (11) and have shown to enhance the resistance of the plans to the fungal infection or stress conditions (6)(7)16). …”

Section: Discussionmentioning

confidence: 99%

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Agrobacterium-Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum

Aghazadeh

Zamani

Motallebi

et al. 2017

Iran. J. Biotechnol.

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Background:Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense system. Objectives: Transformation of the rice tlp into canola via Agrobacterium-mediated transformation and evaluation of the antifungal activity of the expressed TLP in the transgenic events on the S. sclerotiorum growth was subject to investigation. Materials and methods:The canola (R line Hyola308) was used for transformation experiment. The vector, pBITLPRA1, was used for the stable transformation. The PCR and southern blotting techniques were used to confi rm transgene's presence in the transgenic canola events. Antifungal activity of transgenic plants was evaluated by the radial diff usion and spore germination assays. T 2 transgenic plants were evaluated by the intact leaf inoculation method in greenhouse assay. Results: In this study, pBITLPRA1 construct containing tlp gene was introduced into canola and the transformed plants were verifi ed by PCR. The glucanase activity of tlp gene in T 0 generation was measured and transgenic plants with high activity were assessed by Southern blot analysis to confi rm the copy number of the gene. Also, antifungal activity of the single copy T 0 transgenic plants against Sclerotinia sclerotiorum was evaluated by radial diff usion and spore germination assays. In greenhouse assay, evaluation of T 2 transgenic plants by the intact leaf inoculation method demonstrated that following the infection with S. sclerotiorum, there was a signifi cant reduction in the lesion's diameter in transgenic lines compared to the non-transgenic ones. Conclusions: These results revealed that expression of TLP has an inhibitory eff ect against fungus compared to nontransgenic plants both in vitro and in vivo (i.e., greenhouse condition). These transgenic lines could be used as the additional sources of disease resistance for canola breeding program.

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Overexpression of Camellia sinensis Thaumatin-Like Protein, CsTLP in Potato Confers Enhanced Resistance to Macrophomina phaseolina and Phytophthora infestans Infection

Cited by 76 publications

References 66 publications

Transcriptome Analysis of the Melon-Fusarium oxysporum f. sp. melonis Race 1.2 Pathosystem in Susceptible and Resistant Plants

Transcriptome Analysis of the Melon-Fusarium oxysporum f. sp. melonis Race 1.2 Pathosystem in Susceptible and Resistant Plants

Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice

Agrobacterium-Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum

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