Overexpression of caveolins in<i>Caenorhabditis elegans</i>induces changes in egg-laying and fecundity

Parker, Scott F.; Baylis, H. A.

doi:10.4161/cib.2.5.8715

“…Overexpression of CAV-1 in C . elegans protected against the loss of fecundity caused by heat shock, and overexpression of CAV-2 increased both the rate and yield of egg-laying [ 103 ].…”

Section: Discussionmentioning

confidence: 99%

Experience Modulates the Reproductive Response to Heat Stress in C. elegans via Multiple Physiological Processes

Gouvêa

¹

,

Aprison

²

,

Ruvinsky

³

2015

View full text Add to dashboard Cite

Natural environments are considerably more variable than laboratory settings and often involve transient exposure to stressful conditions. To fully understand how organisms have evolved to respond to any given stress, prior experience must therefore be considered. We investigated the effects of individual and ancestral experience on C. elegans reproduction. We documented ways in which cultivation at 15°C or 25°C affects developmental time, lifetime fecundity, and reproductive performance after severe heat stress that exceeds the fertile range of the organism but is compatible with survival and future fecundity. We found that experience modulates multiple aspects of reproductive physiology, including the male and female germ lines and the interaction between them. These responses vary in their environmental sensitivity, suggesting the existence of complex mechanisms for coping with unpredictable and stressful environments.

show abstract

“…An example of a decrease in chromatin accessibility from embryo to L3 can be seen in the promoter region of the cav-1 gene, which is expressed during embryogenesis but not larval development (Parker et al 2009) (Fig. 1D).…”

Section: High-resolution Chromatin Accessibility Profiles From Three mentioning

confidence: 99%

Chromatin accessibility dynamics reveal novel functional enhancers inC. elegans

Daugherty

¹

,

Yeo

²

,

Buenrostro

³

et al. 2016

Preprint

View full text Add to dashboard Cite

Chromatin accessibility, a crucial component of genome regulation, has primarily been studied in homogeneous and simple systems, such as isolated cell populations or early-development models. Whether chromatin accessibility can be assessed in complex, dynamic systems in vivo with high sensitivity remains largely unexplored. In this study, we use ATAC-seq to identify chromatin accessibility changes in a whole animal, the model organism Caenorhabditis elegans, from embryogenesis to adulthood. Chromatin accessibility changes between developmental stages are highly reproducible, recapitulate histone modification changes, and reveal key regulatory aspects of the epigenomic landscape throughout organismal development. We find that over 5000 distal noncoding regions exhibit dynamic changes in chromatin accessibility between developmental stages and could thereby represent putative enhancers. When tested in vivo, several of these putative enhancers indeed drive novel cell-type-and temporal-specific patterns of expression. Finally, by integrating transcription factor binding motifs in a machine learning framework, we identify EOR-1 as a unique transcription factor that may regulate chromatin dynamics during development. Our study provides a unique resource for C. elegans, a system in which the prevalence and importance of enhancers remains poorly characterized, and demonstrates the power of using whole organism chromatin accessibility to identify novel regulatory regions in complex systems.

show abstract

“…1D; Parker and Baylis 2009). Conversely, several ATAC-seq peaks drastically increase from embryo to L3 in the promoter and regions upstream of the daf-12 gene, which is a key regulator of stage-specific developmental programs, particularly at L3 ( Fig.…”

Section: High-resolution Chromatin Accessibility Profiles From Three mentioning

confidence: 99%

Chromatin accessibility dynamics reveal novel functional enhancers in C. elegans

Daugherty

¹

,

Yeo

²

,

Buenrostro

³

et al. 2017

View full text Add to dashboard Cite

Chromatin accessibility, a crucial component of genome regulation, has primarily been studied in homogeneous and simple systems, such as isolated cell populations or early-development models. Whether chromatin accessibility can be assessed in complex, dynamic systems in vivo with high sensitivity remains largely unexplored. In this study, we use ATAC-seq to identify chromatin accessibility changes in a whole animal, the model organism Caenorhabditis elegans, from embryogenesis to adulthood. Chromatin accessibility changes between developmental stages are highly reproducible, recapitulate histone modification changes, and reveal key regulatory aspects of the epigenomic landscape throughout organismal development. We find that over 5000 distal noncoding regions exhibit dynamic changes in chromatin accessibility between developmental stages and could thereby represent putative enhancers. When tested in vivo, several of these putative enhancers indeed drive novel cell-type-and temporal-specific patterns of expression. Finally, by integrating transcription factor binding motifs in a machine learning framework, we identify EOR-1 as a unique transcription factor that may regulate chromatin dynamics during development. Our study provides a unique resource for C. elegans, a system in which the prevalence and importance of enhancers remains poorly characterized, and demonstrates the power of using whole organism chromatin accessibility to identify novel regulatory regions in complex systems.

show abstract

Overexpression of caveolins inCaenorhabditis elegansinduces changes in egg-laying and fecundity

Cited by 4 publications

References 24 publications

Experience Modulates the Reproductive Response to Heat Stress in C. elegans via Multiple Physiological Processes

Experience Modulates the Reproductive Response to Heat Stress in C. elegans via Multiple Physiological Processes

Chromatin accessibility dynamics reveal novel functional enhancers inC. elegans

Chromatin accessibility dynamics reveal novel functional enhancers in C. elegans

Contact Info

Product

Resources

About