Overexpression of CD61 promotes hUC‐MSC differentiation into male germ‐like cells

Li, Bo; Liu, Weishuai; Zhuang, Mengru; Li, Na; Wu, Siyu; S, Pan

doi:10.1111/cpr.12236

Cited by 15 publications

(10 citation statements)

References 43 publications

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“…PGC-like cells, which are positive for SSEA1 and CD61, can develop into spermatid cells19. Our previous studies showed that CD61 promotes hUC-MSCs to differentiate into male germ-like cells12. Nevertheless, the mechanism of CD61 in PGC development remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that MSCs derived from bone marrow and umbilical cord can differentiate into germ-like cells1011. Our previous investigation also demonstrated that human umbilical cord mesenchymal stem cells (hUC-MSCs) can potentially differentiate into germ-like cells under treatment with CD61 and bone morphogenetic protein 4 (BMP4)12. However, the mechanisms remain unclear.…”

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confidence: 99%

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CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

Fang

Wei

et al. 2017

Sci Rep

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Previous studies have shown that CD61 (integrin-β3) promotes the differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into germ-like cells. However, the mechanism remains unclear. In this study, we showed that overexpression of CD61 in canine adipose-derived mesenchymal stem cells (cADMSCs) promotes their differentiation into primordial germ cell (PGC)-like cells. Quantitative real-time PCR, immunocytochemistry and western blot detected higher levels of PGC-specific markers in CD61-overexpressed cADMSCs compared with those in control cells. Moreover, phosphorylation of Smad2, a downstream mediator of transforming growth factor beta (TGF-β), was increased in CD61-overexpressed cADMSCs than that in control cells. However, the expression of PGC-specific markers was downregulated in cADMSCs treated with a TGF-β inhibitor. These results suggested that CD61 could induce cADMSCs to differentiate into PGC-like cells by relying on the activation of TGF-β pathway. ADMSCs possess a considerable potential in treating the infertility of rare animal species.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

Fang

Wei

et al. 2017

Sci Rep

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“…Molecular evaluation on day 7 post-treatment with BMP4 revealed that the expression of some GC-specific markers like SSEA-1, Prdm1, Prdm14, Ap2c, Stella, Sox2, C-kit, Stra8, Scp3 and Prm1 were upregulated in the transfected cells far more than in the non-transfected BMP4 treated human UC-MSCs. Although the researchers claimed that they saw a number of spermatid-like cells, which expressed post meiotic marker, Acr, they did not show further evidence such as the chromosomal content and fertilizing capacity of the cells [44].…”

Section: Umbilical Cord (Uc)-mscsmentioning

confidence: 97%

Functional Germ Cells From Non-Testicular Adult Stem Cells: A Dream or Reality?

Ghasemzadeh-Hasankolaei

Eslaminejad

Ghasemzadeh-Hasankolaei

2017

CSCR

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“…Fang et al . and Li et al proved that CD61 could promote the differentiation of ADMSC into PGC-like cells through activation of the TGF-β pathway ( Li et al, 2016 ; Fang et al, 2017 ). Wei et al .…”

Section: The Mechanism Of Treating Pof With Mscsmentioning

confidence: 99%

Research Progress on the Treatment of Premature Ovarian Failure Using Mesenchymal Stem Cells: A Literature Review

Wang

Liu

et al. 2021

Front. Cell Dev. Biol.

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Premature ovarian failure (POF) has become one of the main causes of infertility in women of childbearing age and the incidence of POF is increasing year by year, seriously affecting the physical and mental health of patients and increasing the economic burden on families and society as a whole. The etiology and pathogenesis of POF are complex and not very clear at present. Currently, hormone replacement therapy is mainly used to improve the symptoms of low estrogen, but cannot fundamentally solve the fertility problem. In recent years, stem cell (SC) transplantation has become one of the research hotspots in the treatment of POF. The results from animal experiments bring hope for the recovery of ovarian function and fertility in patients with POF. In this article, we searched the published literature between 2000 and 2020 from the PubMed database (https://pubmed.ncbi.nlm.nih.gov), and summarized the preclinical research data and possible therapeutic mechanism of mesenchymal stem cells (MSCs) in the treatment of POF. Our aim is to provide useful information for understanding POF and reference for follow-up research and treatment of POF.

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Overexpression of CD61 promotes hUC‐MSC differentiation into male germ‐like cells

Abstract: Under induction of BMP4, CD61-overexpressing hUC-MSCs, which had turned into PGC-like cells, could be further differentiated into male germ-like cells. Thus, a simple and efficient approach to study male germ cell development by using hUC-MSCs has been established.

Cited by 15 publications

References 43 publications

CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

Functional Germ Cells From Non-Testicular Adult Stem Cells: A Dream or Reality?

Research Progress on the Treatment of Premature Ovarian Failure Using Mesenchymal Stem Cells: A Literature Review

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