Overexpression of circRNA_100290 promotes the progression of laryngeal squamous cell carcinoma through the miR-136-5p/RAP2C axis

Wang, Zhenxiao; Huang, Chaoping; Zhang, Aobo; Lu, Cheng Hsien; Liu, Liangfa

doi:10.1016/j.biopha.2020.109874

Cited by 35 publications

(30 citation statements)

References 38 publications

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“…Functional experiments indicate that miR-136 can inhibit cell proliferation, invasion and metastasis and induce apoptosis during cancer progression (15,35,36). In particular, it has been reported that downregulation of miR-136-5p markedly reduces cell viability, invasion and migration in LSCC (37). Consistent with the previous results, the present study identified the suppressive functions of miR-136-5p on cell viability, invasion and migration during LSCC and HPSCC progression.…”

Section: Discussionsupporting

confidence: 90%

The miR‑136‑5p/ROCK1 axis suppresses invasion and migration, and enhances cisplatin sensitivity in head and neck cancer cells

et al. 2021

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Laryngeal squamous cell carcinoma (LSCC) and hypopharyngeal squamous cell carcinoma (HPSCC) are two types of head and neck cancers with high incidence rates and relatively poor prognoses. The aim of the present study was to determine the effects of microRNA (miR/miRNA)-136-5p and its downstream target, Rho-associated coiled-coil containing protein kinase 1 (ROCK1), on LSCC and HPSCC progression and cisplatin sensitivity. The miRNA and protein expression levels in head and neck cancer cell lines were evaluated using reverse transcription-quantitative PCR and western blotting, respectively. MTT, wound healing assays, transwell assays and flow cytometry analysis were performed to measure cell properties. The binding between miR-136-5p and ROCK1 was detected using a dual-luciferase reporter assay. Autophagy double-labeled adenoviral infection assays were used to assess cell autophagy. The results showed that miR-136-5p was expressed in LSCC and HPSCC cells. Functional experiments showed that the expression of miR-136-5p in LSCC and HPSCC cells was negatively correlated with cell viability, invasion and migration. Additionally, miR-136-5p overexpression inhibited epithelial-mesenchymal transition, whereas miR-136-5p knockdown had the opposite effect. Dual-luciferase reporter assays confirmed the targeting relationship between miR-136-5p and ROCK1. miR-136-5p overexpression increased the cisplatin sensitivity of LSCC and HPSCC cells by reducing cell viability, as well as promoting cell apoptosis and autophagy. miR-136-5p overexpression decreased the expression levels of its downstream target ROCK1 and attenuated activity of the Akt/mTOR signaling pathway in cisplatin-treated LSCC and HPSCC cells. Conversely, miR-136-5p knockdown increased ROCK1 levels and decreased cisplatin sensitivity of the LSCC and HPSCC cells by increasing cell viability and inhibiting cell apoptosis, which was reversed by ROCK1 inhibition using the ROCK1 inhibitor, Y27632. Taken together, the results showed that the miR-136-5p/ROCK1 axis inhibits cell invasion and migration, and increases the sensitivity of LSCC and HPSCC cells to cisplatin.

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Section: Discussionsupporting

confidence: 90%

The miR‑136‑5p/ROCK1 axis suppresses invasion and migration, and enhances cisplatin sensitivity in head and neck cancer cells

et al. 2021

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“…165, 915, and 364 unique microRNAs were predicted to have putative binding sites at 3′UTRs of DHX36 , OPA1 , and SENP2 , respectively (Supplementary Table 2). Venn diagram showed that DHX36 , OPA1 , and SENP2 shared 16 microRNAs as predicted by online tools, which includes miR‐33a, 30 miR‐205, 31 miR‐125, 32 miR‐150, 33 and miR‐136 34 that were characterized as tumor suppressors in HNSC as well as other cancers (Figure 6A,B, Supplementary Figure 3A). We then determined the common experimentally validated microRNAs targeting DHX36 , OPA1 , and SENP2 using miRTarBase and miRWalk 2.0 (Figure 6C, Supplementary Table 3), and found that these validated microRNAs had a tendency to negatively correlate with DHX36 , OPA1 , and SENP2 , although not significant, in HNSC tumor samples (DHX36 vs miR‐30a‐5p, R = −0.090; OPA1 vs miR‐375, R = −0.176; SENP2 vs miR‐149‐3p, R = −0.104, Figure 6D).…”

Section: Resultsmentioning

confidence: 95%

Comprehensive in silico analysis for identification of novel candidate target genes, including DHX36, OPA1, and SENP2, located on chromosome 3q in head and neck cancers

et al. 2020

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Background: Major milestones of head and neck carcinogenesis have been associated with various genetic abnormalities; however, a clear picture of the molecular networks deregulated during the carcinogenesis of head and neck squamous cell carcinoma (HNSC) has not yet completely revealed. Methods: In this study, we used in silico tools and online data sets to evaluate the underlying reasons for the expressional changes of genes residing within the chromosome 3q and to help understanding their contributions to HNSC carcinogenesis. Results: We found that 13 of 20 most upregulated genes in HNSC are localized to 3q. Further analysis revealed a gene signature consisting of DHX36, OPA1, and SENP2, which showed significant correlation in HNSC samples and potentially be deregulated through similar mechanisms including DNA amplification, transcriptional, and posttranscriptional regulation. Conclusions: Considering our findings, we suggest DHX36, OPA1, and SENP2 genes as overexpressed in HNSC tumors and that might be concurrently involved in HNSC carcinogenesis, tumor progression, and induction of angiogenic pathways.

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“…Multiple circRNAs have been demonstrated to be involved in HNC development through the regulation of the proliferation process ( Figure 1 ). CircATRNL1 ( 40 ), hsa_circ_0007059 ( 41 ), circRNA_002178 ( 42 ), hsa_circ_0046263 ( 43 ), hsa_circ_0005379 ( 44 ), circUHRF1 ( 45 ), circPVT1 ( 46 , 47 ), circHIPK3 ( 48 , 49 ), circRNA_0000140 ( 50 ), circRNA_100290 ( 51 ), circDOCK1 ( 52 ), hsa_circ_0055538 ( 53 ), circITCH ( 54 ), hsa_circRNA_101036 ( 55 ), circCCND1 ( 20 ), circMYLK ( 56 ), CDR1as ( 17 , 57 , 58 ), circPKD2 ( 59 ), circLARP4 ( 60 ), hsa_circ_0042666 ( 61 ), hsa_circRNA_100533 ( 62 ), circMDM2 ( 63 ), circ_0067934 ( 64 ), circRPMS1 ( 65 ), circ-ZNF609 ( 66 , 67 ), hsa_circ_0072387 ( 68 ), circRASSF2 ( 38 ), hsa_circ_0023028 ( 69 ), circCTDP1 ( 70 ), circ_0008450 ( 71 ), circMATR3 ( 72 ), hsa_circ_0057481 ( 73 ), circ_0001742 ( 74 ), circ_0000218 ( 75 ), circ_0001971 ( 76 ), circCORO1C ( 77 ), hsa_circ_0109291 ( 78 ), hsa_circ_0036722 ( 34 ), hsa_circ_0002203 ( 79 ) and hsa_circ_0066755 ( 80 ) are associated with tumor proliferation ( Table 1 ).…”

Section: Circrnas Are Associated With Proliferation In Hncmentioning

confidence: 99%

“…CircRNAs in HNC have important roles in HNC metastasis and invasion ( Figure 2 ). Hsa_circ_0007059 ( 41 ), circUHRF1 ( 45 ), circHIPK3 ( 48 ), circRNA_0000140 ( 50 ), circRNA_100290 ( 51 ), hsa_circ_0055538 ( 53 ), CDR1as ( 17 , 57 ), circPKD2 ( 59 ), circLARP4 ( 60 ), hsa_circ_0042666 ( 61 ), hsa_circRNA_100533 ( 62 ), circ_0067934 ( 64 ), circRPMS1 ( 65 ), circ-ZNF609 ( 66 ), hsa_circ_0072387 ( 68 ), circRASSF2 ( 38 ), hsa_circ_0023028 ( 69 ), circCTDP1 ( 70 ), circ_0008450 ( 71 ), circMATR3 ( 72 ), hsa_circ_0057481 ( 73 ), circ_0001742 ( 74 ), circCORO1C ( 77 ), hsa_circ_0004491 ( 37 ), circ_0001971 ( 33 ), circFLNA ( 99 ), hsa_circ_0001162 ( 100 ), circARHGAP12 ( 101 ), circCRIM1 ( 102 ), circEPSTI1 ( 103 ), hsa_circ_0109291 ( 78 ), circRNA_002178 ( 42 ), hsa_circ_0002203 ( 79 ) and hsa_circ_0066755 ( 80 ) are involved in the regulation of HNC invasion and metastasis ( Table 2 ).…”

Section: Circrnas Are Associated With Invasion and Metastasis In Hncmentioning

confidence: 99%

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Research Progress of circRNAs in Head and Neck Cancers

Deng

et al. 2021

Front. Oncol.

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Circular RNAs (circRNAs) are a novel type of non-coding RNAs. Because of their characteristics of a closed loop structure, disease- and tissue-specificity, and high conservation and stability, circRNAs have the potential to be biomarkers for disease diagnosis. Head and neck cancers are one of the most common malignant tumors with high incidence rates globally. Affected patients are often diagnosed at the advanced stage with poor prognosis, owing to the concealment of anatomic sites. The characteristics, functions, and specific mechanisms of circRNAs in head and neck cancers are increasingly being discovered, and they have important clinical significance for the early diagnosis, treatment, and prognosis evaluation of patients with cancer. In this study, the generation, characteristics, and functions of circRNAs, along with their regulatory mechanisms in head and neck cancers have been summarized. We report that circRNAs interact with molecules such as transcription and growth factors to influence specific pathways involved in tumorigenesis. We conclude that circRNAs have an important role to play in the proliferation, invasion, metastasis, energy and substance metabolism, and treatment resistance in cancers.

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Overexpression of circRNA_100290 promotes the progression of laryngeal squamous cell carcinoma through the miR-136-5p/RAP2C axis

Cited by 35 publications

References 38 publications

The miR‑136‑5p/ROCK1 axis suppresses invasion and migration, and enhances cisplatin sensitivity in head and neck cancer cells

The miR‑136‑5p/ROCK1 axis suppresses invasion and migration, and enhances cisplatin sensitivity in head and neck cancer cells

Comprehensive in silico analysis for identification of novel candidate target genes, including DHX36, OPA1, and SENP2, located on chromosome 3q in head and neck cancers

Research Progress of circRNAs in Head and Neck Cancers

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