Overexpression of cotton genes GhDIR4 and GhPRXIIB in Arabidopsis thaliana improves plant resistance to root-knot nematode (Meloidogyne incognita) infection

Santos, Cristiane dos; Carmo, Lílian S.T.; Távora, Fabiano Touzdjian Pinheiro Kohlrausch; Lima, Roberta Ferreira Cruz de; Mendes, Pollyana da Nobrega; Labuto., Luciana B. D.; Sá, Maria Eugênia L. de; Grossi-de-Sá, Maria Fátima; Mehta, Ângela

doi:10.1007/s13205-022-03282-4

Cited by 5 publications

(5 citation statements)

References 40 publications

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“…The ability to scavenge ROS and prevent oxidative damage is crucial for plant defense against necrotrophic pathogens like V. dahliae . Our findings are in line with previous studies demonstrating the importance of antioxidant defenses in governing cotton's interaction with V. dahliae [ 33 , 34 ]. The upregulation of antioxidant systems, controlled in part by defense-associated transcription factors like ERFs, is likely a key component of the enhanced tolerance we observed in the GauERF105 overexpression lines.…”

Section: Discussionsupporting

confidence: 93%

Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton

Wang,

Umer,

Cai

et al. 2023

BMC Plant Biol

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Background The cotton industry suffers significant yield losses annually due to Verticillium wilt, which is considered the most destructive disease affecting the crop. However, the precise mechanisms behind this disease in cotton remain largely unexplored. Methods Our approach involved utilizing transcriptome data from G. australe which was exposed to Verticillium dahliae infection. From this data, we identified ethylene-responsive factors and further investigated their potential role in resistance through functional validations via Virus-induced gene silencing (VIGS) in cotton and overexpression in Arabidopsis. Results A total of 23 ethylene response factors (ERFs) were identified and their expression was analyzed at different time intervals (24 h, 48 h, and 72 h post-inoculation). Among them, GauERF105 was selected based on qRT-PCR expression analysis for further investigation. To demonstrate the significance of GauERF105, VIGS was utilized, revealing that suppressing GauERF105 leads to more severe infections in cotton plants compared to the wild-type. Additionally, the silenced plants exhibited reduced lignin deposition in the stems compared to the WT plants, indicating that the silencing of GauERF105 also impacts lignin content. The overexpression of GauERF105 in Arabidopsis confirmed its pivotal role in conferring resistance against Verticillium dahliae infection. Our results suggest that WT possesses higher levels of the oxidative stress markers MDA and H2O2 as compared to the overexpressed lines. In contrast, the activities of the antioxidant enzymes SOD and POD were higher in the overexpressed lines compared to the WT. Furthermore, DAB and trypan staining of the overexpressed lines suggested a greater impact of the disease in the wild-type compared to the transgenic lines. Conclusions Our findings provide confirmation that GauERF105 is a crucial candidate in the defense mechanism of cotton against Verticillium dahliae invasion, and plays a pivotal role in this process. These results have the potential to facilitate the development of germplasm resistance in cotton.

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Section: Discussionsupporting

confidence: 93%

Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton

Wang,

Umer,

Cai

et al. 2023

BMC Plant Biol

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“…Comparatively, the overexpression lines exhibited lower MDA and H 2 O 2 levels, accompanied by higher SOD and POD activities when compared to wildtype plants (Figure 9d‐g). Our findings align with prior research that highlights the critical role of antioxidant defense in governing the interaction between cotton plants and V. dahliae (Dos Santos et al, 2022; Zhang et al, 2022). Additionally, DAB staining results revealed a reduced staining intensity in transgenic Arabidopsis leaves in contrast to the more pronounced staining observed in wildtype plants (Figure 9h).…”

Section: Discussionsupporting

confidence: 92%

Unravelling the Functional Role of GthGAPC2 in Cotton's Defense Against Verticillium dahliae through Proteome

Umer,

Batool,

Yang

et al. 2023

Physiologia Plantarum

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Cotton (Gossypium spp.) is an economically important crop, but its productivity is often hindered by the soil‐borne pathogen Verticillium dahliae. This study aimed to investigate the response of cotton roots to V. dahliae infection by analysing the proteome of Gossypium thurberi (resistant) and Gossypium raimondii (susceptible) at 0 h, 24 h, and 48 h post‐infection. Through weighted protein coexpression network analysis, fifteen hub proteins crucial for defense against V. dahliae were identified. Expression analysis revealed the pivotal role of GthGAPC2, encoding GLYCERALDEHYDE‐3‐PHOSPHATE DEHYDROGENASE 2, in conferring resistance to V. dahliae in cotton. Virus‐induced gene Silencing (VIGS) of GthGAPC2 increased susceptibility to V. dahliae, which was supported by oxidant and antioxidant enzyme activities. Furthermore, GthGAPC2 silencing influenced lignin content, indicating its involvement in lignin biosynthesis regulation. Transient overexpression of GthGAPC2 in tobacco supported its role in cell death processes. Subcellular localization studies showed predominant nuclear localization of GthGAPC2. Overexpression of GthGAPC2 in Arabidopsis also confirms its significant role in V. dahliae resistance. These findings shed light on the molecular mechanisms of disease resistance in Gossypium thurberi. Identification of GthGAPC2, as a key protein involved in V. dahliae resistance, and its functional implications can aid breeding strategies for enhancing cotton's disease resistance.

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“…Considering the potential role of DIRIGENTs in plant response to environmental constraints (Paniagua et al ., 2017; dos Santos et al ., 2022; Li et al ., 2022), we inspected the possible role of DIR13 in the salt stress response. When placed on control media (0 mM NaCl), the germination rate of the DIR13 OE or DIR13 -deficient lines was similar to Col-0 WT (Supplemental Figure 6A, D).…”

Section: Resultsmentioning

confidence: 99%

“…DIR13 (At4G11190) is the closest paralog of DIR5, DIR6, DIR14, and DIR12 but lacks the conserved residues necessary for (-)-pinoresinol formation and exhibits strong, although not exclusive, expression in the root (Kim et al ., 2012). DIR genes are induced by biotic and abiotic stresses (Paniagua et al ., 2017) and overexpression of some DIRs can lead to improved tolerance to biotic and abiotic stresses (dos Santos et al ., 2022; Li et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

Cytokinin-inducibleDIRIGENT13involved in lignan synthesis and ROS accumulation promotes root growth and abiotic stress tolerance inArabidopsis

Melnikava,

Perreau,

Paniagua

et al. 2024

Preprint

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Dirigent (DIR) proteins mediate regio- and stereoselectivity of phenoxy radical coupling of monolignols for (neo)lignan or lignin synthesis. However, the role of DIR proteins and lignans in plant development and response to environmental stresses remains elusive. Here, we provide a functional characterization of the cytokinin-responsiveDIRIGENT13(DIR13) inArabidopsis thaliana. DIR13 was localized to the root endodermis and at the margins of lateral root primordia and lateral roots. While not necessary for Casparian strip formation, DIR13 promoted both main and lateral root growth. Untargeted metabolomics and imaging mass spectrometry analyses unveiled the role of DIR13 in facilitating (neo)lignan synthesis in primary and lateral roots. Interestingly, DIR13 activated the production of putative oxomatairesinol and matairesinol-cysteine which are oxidative derivatives of the lignan matairesinol. Our data also identified DIR13 as an enhancer of salt and drought tolerance. Particularly,DIR13attenuated salt stress-mediated inhibition of germination and root growth. Moreover, DIR13 activated reactive oxygen species (ROS) accumulation both under control and salt stress conditions, and cytokinin further enhanced the salt-induced ROS production specifically in theDIR13overexpressing line. Our results uncover a role for DIR13-produced lignans in the priming of ROS accumulation, mediating both abiotic stress tolerance and the control of root architecture.

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Overexpression of cotton genes GhDIR4 and GhPRXIIB in Arabidopsis thaliana improves plant resistance to root-knot nematode (Meloidogyne incognita) infection

Cited by 5 publications

References 40 publications

Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton

Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton

Unravelling the Functional Role of GthGAPC2 in Cotton's Defense Against Verticillium dahliae through Proteome

Cytokinin-inducibleDIRIGENT13involved in lignan synthesis and ROS accumulation promotes root growth and abiotic stress tolerance inArabidopsis

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