Overexpression of CsSnRK2.5 increases tolerance to drought stress in transgenic Arabidopsis

Zhang, Yongheng; Wan, Siqin; Liu, Xianghong; He, Jingyuan; Cheng, Long; Duan, Minghui; Liu, Huan; Wang, Weidong; Yu, Youben

doi:10.1016/j.plaphy.2020.02.035

Cited by 19 publications

(12 citation statements)

References 54 publications

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“…For stomatal aperture assay, rosette leaves of four-week-old Arabidopsis seedlings were collected and incubated in 200 mL stomatal closure solution (10 mM KCl, 1 mM CaCl 2 , 25 mM MES) for 2 h [ 52 ]. Photographs were taken using an optical microscope (Hitachi, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

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The WRKY gene family, which is one of the largest transcription factor (TF) families, plays an important role in numerous aspects of plant growth and development, especially in various stress responses. However, the functional roles of the WRKY gene family in loquat are relatively unknown. In this study, a novel WRKY gene, EjWRKY17, was characterized from Eriobotrya japonica, which was significantly upregulated in leaves by melatonin treatment during drought stress. The EjWRKY17 protein, belonging to group II of the WRKY family, was localized in the nucleus. The results indicated that overexpression of EjWRKY17 increased cotyledon greening and root elongation in transgenic Arabidopsis lines under abscisic acid (ABA) treatment. Meanwhile, overexpression of EjWRKY17 led to enhanced drought tolerance in transgenic lines, which was supported by the lower water loss, limited electrolyte leakage, and lower levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Further investigations showed that overexpression of EjWRKY17 promoted ABA-mediated stomatal closure and remarkably up-regulated ABA biosynthesis and stress-related gene expression in transgenic lines under drought stress. Overall, our findings reveal that EjWRKY17 possibly acts as a positive regulator in ABA-regulated drought tolerance.

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Section: Methodsmentioning

confidence: 99%

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

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“…Transgenic Arabidopsis presented with elevated dehydrin levels compared to WT plants. Therefore, CsSnRK2.5 is a positive regulator of ABA-mediated dehydrin expression [ 55 ]. VvABF2 is a homolog of AREB/ABFs from Arabidopsis that was isolated from grapevine and constitutively expressed in Arabidopsis.…”

Section: Dehydrin Gene Expression Regulatory Networkmentioning

confidence: 99%

Plant Dehydrins: Expression, Regulatory Networks, and Protective Roles in Plants Challenged by Abiotic Stress

Sun

Chen

et al. 2021

IJMS

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Dehydrins, also known as Group II late embryogenesis abundant (LEA) proteins, are classic intrinsically disordered proteins, which have high hydrophilicity. A wide range of hostile environmental conditions including low temperature, drought, and high salinity stimulate dehydrin expression. Numerous studies have furnished evidence for the protective role played by dehydrins in plants exposed to abiotic stress. Furthermore, dehydrins play important roles in seed maturation and plant stress tolerance. Hence, dehydrins might also protect plasma membranes and proteins and stabilize DNA conformations. In the present review, we discuss the regulatory networks of dehydrin gene expression including the abscisic acid (ABA), mitogen-activated protein (MAP) kinase cascade, and Ca2+ signaling pathways. Crosstalk among these molecules and pathways may form a complex, diverse regulatory network, which may be implicated in regulating the same dehydrin.

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“…In combined stress, kinases like serine kinase (Os05g0466900, Os12g0454800) were upregulated, similarly leucine-rich repeat genes (Os01g0162300, Os01g0162500), ras-related protein (Os01g0750000), MTN3 (Os01g0606000), phosphofructokinase (Os06g0326400), cyclin-dependent kinase inhibitor (Os09g0459900) genes were upregulated. Overexpression of serine/threonine kinases showed improved abiotic stress tolerance in Arabidopsis [ 25 ]. The role of Os12g0454800 in cytokinin signalling has been deciphered [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic responses under combined bacterial blight and drought stress in rice reveal potential genes to improve multi-stress tolerance

et al. 2022

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Background The unprecedented drought and frequent occurrence of pathogen infection in rice is becoming more due to climate change. Simultaneous occurrence of stresses lead to more crop loss. To cope up multiple stresses, the durable resistant cultivars needs to be developed, by identifying relevant genes from combined biotic and abiotic stress exposed plants. Results We studied the effect of drought stress, bacterial leaf blight disease causing Xanthomonas oryzae pv. oryzae (Xoo) pathogen infection and combined stress in contrasting BPT5204 and TN1 rice genotypes. Mild drought stress increased Xoo infection irrespective of the genotype. To identify relevant genes that could be used to develop multi-stress tolerant rice, RNA sequencing from individual drought, pathogen and combined stresses in contrasting genotypes has been developed. Many important genes are identified from resistant genotype and diverse group of genes are differentially expressed in contrasting genotypes under combined stress. Further, a meta-analysis from individual drought and Xoo pathogen stress from public domain data sets narrowed- down candidate differentially expressed genes. Many translation associated genes are differentially expressed suggesting their extra-ribosomal function in multi-stress adaptation. Overexpression of many of these genes showed their relevance in improving stress tolerance in rice by different scientific groups. In combined stress, many downregulated genes also showed their relevance in stress adaptation when they were over-expressed. Conclusions Our study identifies many important genes, which can be used as molecular markers and targets for genetic manipulation to develop durable resistant rice cultivars. Strategies should be developed to activate downregulated genes, to improve multi-stress tolerance in plants.

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Overexpression of CsSnRK2.5 increases tolerance to drought stress in transgenic Arabidopsis

Cited by 19 publications

References 54 publications

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Plant Dehydrins: Expression, Regulatory Networks, and Protective Roles in Plants Challenged by Abiotic Stress

Transcriptomic responses under combined bacterial blight and drought stress in rice reveal potential genes to improve multi-stress tolerance

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