Overexpression of cytoplasmic dynamin 2 is associated with worse outcomes in patients with clear cell renal cell carcinoma

Abstract

BACKGROUND: Dynamin 2 (DNM2) involved in tumor progression in various malignancies. OBJECTIVE: For the first time, we evaluated DNM2 expression pattern, its association with clinicopathological characteristics and survival outcomes in RCC subtypes. METHODS: We evaluated the DNM2 expression pattern in RCC tissues as well as adjacent normal tissue using immunohistochemistry on tissue microarray (TMA) slides. RESULTS: Our findings revealed increased DNM2 expression in RCC samples rather than in adjacent normal tissues. The results indicated that there was a statistically significant difference between cytoplasmic expression of DNM2 among subtypes of RCC in terms of intensity of staining, percentage of positive tumor cells, and H-score (P= 0.024, 0.049, and 0.009, respectively). The analysis revealed that increased cytoplasmic expression of DNM2 in ccRCC is associated with worse OS (log rank: P= 0.045), DSS (P= 0.049), and PFS (P= 0.041). Furthermore, cytoplasmic expression of DNM2 was found as an independent prognostic factor affecting DSS and PFS in multivariate analysis. CONCLUSIONS: Our results indicated that DNM2 cytoplasmic expression is associated with tumor aggressiveness and poor outcomes. DNM2 could serve as a promising prognostic biomarker and therapeutic target in patients with ccRCC.

Cellular elasticity in cancer: a review of altered biomechanical features

“…94 In a variety of solid tumors, matrix stiffness-a mechanical property of the ECM increases in a gradient pattern from the tumor core to the tumor periphery and can stimulate treatment resistance, invasion, proliferation, metastasis, and recurrence. 95 The stiffness and elasticity of ECM vary from tissue to tissue. The ECM's elasticity can vary by several orders of magnitude.…”

Nuclear overexpression of DNA damage-inducible transcript 4 (DDIT4) is associated with aggressive tumor behavior in patients with pancreatic tumors

“…DDIT4 expression was evaluated using the IHC method as previously described 80,81 . First, all TMA sections were deparaffinized at 60 °C for 40 min and then rehydrated with xylene and graded ethylic alcohol.…”

Editorial: Pulmonary fibrosis and lung carcinogenesis: do myofibroblasts and cancer-associated fibroblasts share a common identity?

“…Despite the origin of IPF is still unknown, similarly to LC, studying the cellular proliferative mechanisms, considered a key process leading to the lung tissue fibrotic transformation (10), might lead to discover new effective anti-fibrotic agents. In this light, there are currently two antifibrotic/antiproliferative drugs registered and used as a therapeutic strategy to treat patients with IPF, with the aim to slow-down the respiratory function decline and potentially to improve prognosis in the long-term (13,14) As recently emphasized, changes within the ECM proteome (matrisome), composed of the "core" ECM proteins (collagens, glycoproteins and proteoglycans of the ECM structure) and of ECM-associated proteins (mucins, enzymes, cytokines) affect lung tissue architecture, causing lung diseases and tumor development (15)(16)(17)(18).…”