Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants

Kishor, P. B. Kavi; Hong, Zonglie; Miao, Guo-Hua; Hu, Caa.; Verma, D. P. S.

doi:10.1104/pp.108.4.1387

Cited by 1,215 publications

(682 citation statements)

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“…These results suggest that GA pretreatment may have partly removed the water-stress block to germination, which might be due to the fact that GA may induce production and activation of a-amylase and protease in the endosperm; this would result in improved germination capacities (Jones and Carbonell 1984). Accumulation of proline under water stress protected the cell by balancing the osmotic strength of cytosol; preserving protein structures and enzyme activities; scavenging hydroxyl and other free radicals; and regulating cytosol acidity induced by unfavorable environmental conditions (Kishor et al 1995). We found a significantly higher proline accumulation under water stress in both distilled-water-and GA-pretreated seeds (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress

et al. 2009

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Germination of dragon spruce (Picea asperata Mast.) seeds pretreated with gibberellin (GA) in response to water stress and changes in the levels of osmotic adjustments as well as in activities of antioxidant enzymes were investigated. With decreasing water potential caused by increasing concentrations of PEG 6000, germination percentage and germination index decreased gradually; the decrease was especially prominent under the serious water stress from PEG -0.6 MPa. In contrast, osmotic regulation substances (free proline and soluble sugar contents), lipid peroxidation (MDA), and activities of antioxidant enzyme (ascorbate peroxidase, catalase, and peroxidase) increased markedly with decreased water potential. Similarly, the values in all parameters under -0.6 MPa PEG treatment were markedly higher than the control and -0.2 MPa PEG treatment. These results suggested that P. asperata seed germination was insensitive to water stress. In addition, seeds pretreated with GA had increased tolerance to water stress as measured by germination percentage and germination index, osmotic regulation substance, and antioxidant enzyme activities.

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Section: Discussionmentioning

confidence: 99%

Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress

et al. 2009

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“…Recently, results from metabolic engineering experiments have confirmed the functional roles for compatible osmolytes, such as mannital, proline, and betaine, in salt adaptation of high plants [1], [2], [3]. These compounds are presumed to function both in osmoprotection and osmotic adjustment.…”

Section: Introductionmentioning

confidence: 93%

HAL1 mediate salt adaptation in Arabidopsis thaliana

Yang

Zhang

et al. 2001

Cell Res

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The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. Several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K + and Na + showed that transgenic lines were able to retain less Na + than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.

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“…Segundo a literatura (Romagopal, 1986;Broetto et al, 1995;Willadino et al, 1996), a variação de prolina encontrada quando os calos são submetidos a níveis de estresse salino entre 0 e 300 mmol L -1 de NaCl é de 13 a 40 µmol mg -1 de peso fresco sendo esta acumulação considerada como parte do processo de adaptação ao estresse (Djilianov et al, 1997;Mattioni et al, 1997). Atualmente, sabe-se que o acúmulo de prolina está relacionado com a tolerância ao estresse osmótico, e que é controlado por genes que codificam as enzimas da biossintese da prolina (Kavi Kishor et al, 1995).…”

Section: Resultsunclassified

Crescimento de calos embriogênicos de milho submetidos ao estresse salino<A NAME="top"></A>

Bezerra

Willadino

Câmara

2001

Sci. agric. (Piracicaba, Braz.)

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RESUMO: A salinidade do solo é um problema muito importante em áreas irrigadas do semi-árido brasileiro, onde o milho é uma cultura de subsistência. Este trabalho teve como objetivo estudar o efeito de concentrações de NaCl sobre calos embriogênicos, da cultivar Jatinã C3 Anão, uma importante cultivar de milho no Nordeste do Brasil. A indução dos calos foi feita a partir de embriões imaturos assepticamente retirados das sementes e inoculados em meio de cultura N6 suplementado com 2 mg L -1 de 2,4-D. Os calos embriogênicos foram submetidos a diferentes concentrações de cloreto de sódio (0; 50; 100; 150 e 200 mmol L -1 de NaCl), com três repetições durante 60 dias. Os calos submetidos a 100 mmol L -1 de NaCl apresentaram aumento no conteúdo de prolina na ordem de 37% quando comparado com o nível de 50 mmol L -1 de NaCl, sugerindo um efeito positivo da acumulação deste aminoácido em resposta ao estresse salino. Por outro lado, em elevadas concentrações salinas (150 e 200 mmol L -1 ) observou-se decréscimo no crescimento dos calos, bem como redução nos teores de prolina, provavelmente em função de um aumento da fração não embriogênica dos calos. Palavras-chave: Zea mays, salinidade, embriogênese somática, prolina EMBRYOGENIC CALLUS GROWTH OF MAIZE SUBMITTED TO SALT STRESSABSTRACT: Soil salinity is a very important problem in irrigated areas of the brazilian semi-arid area, where maize is a subsistence crop. To study the effect of NaCl concentrations in embryogenic calli of Jatinã C3 Anão, an important maize cultivar of the Northest of Brazil, calli were induced using immature embryos aseptically removed from the seeds and grown on N6 medium supplemented with 2 mg L -1 of 2,4-D. To study the effect of salinity in vitro, embryogenic calli were subjected to different concentrations of sodium chloride (0; 50; 100; 150 and 200 mmol L -1 of NaCl), in three replicates, during 60 days. Calli subjected up to100 mmol L -1 NaCl showed proline concentration 37% higher than callus treated with 50 mmol L -1 NaCl. This result suggests a positive effect of proline accumulation at salt stress conditions. However, at the highest concentration tested (150-200 mmol L -1 NaCl) as lower growth and also a lower proline accumulation was observed, probably due to an increase of the non embryogenic fraction of calli.

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Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants

Cited by 1,215 publications

References 32 publications

Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress

Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress

HAL1 mediate salt adaptation in Arabidopsis thaliana

Crescimento de calos embriogênicos de milho submetidos ao estresse salino<A NAME="top"></A>

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