Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants

Takabe, Tomoko; Uchida, Akio; Shinagawa, Fumi; Terada, Yasutaka; Kajita, Hiroshi; Tanaka, Yoshito; Takabe, Teruhiro; Hayashi, Takahisa; Kawai, Takayoshi; Takabe, Tetsuko

doi:10.1007/s10725-008-9306-3

Cited by 20 publications

(6 citation statements)

References 26 publications

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“…However, not much success has been achieved because of the crossing barriers between different species, especially woody species. Therefore, genetic engineering has been used as an alternative strategy to breed trees with improved salt tolerance (Hu et al, 2005;Takabe et al, 2008;Wang et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants

Yang

Tang

Jiang

et al. 2015

Plant Biotechnology Journal

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SummaryIn higher plants, the salt overly sensitive (SOS) signalling pathway plays a crucial role in maintaining ion homoeostasis and conferring salt tolerance under salinity condition. Previously, we functionally characterized the conserved SOS pathway in the woody plant Populus trichocarpa. In this study, we demonstrate that overexpression of the constitutively active form of PtSOS2 (PtSOS2TD), one of the key components of this pathway, significantly increased salt tolerance in aspen hybrid clone Shanxin Yang (Populus davidiana 9 Populus bolleana). Compared to the wild-type control, transgenic plants constitutively expressing PtSOS2TD exhibited more vigorous growth and produced greater biomass in the presence of high concentrations of NaCl. The improved salt tolerance was associated with a decreased Na + accumulation in the leaves of transgenic plants. Further analyses revealed that plasma membrane Na + /H + exchange activity and Na + efflux in transgenic plants were significantly higher than those in the wild-type plants. Moreover, transgenic plants showed improved capacity in scavenging reactive oxygen species (ROS) generated by salt stress. Taken together, our results suggest that PtSOS2 could serve as an ideal target gene to genetically engineer salt-tolerant trees.

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Section: Discussionmentioning

confidence: 99%

Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants

Yang

Tang

Jiang

et al. 2015

Plant Biotechnology Journal

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“…Similarly, heterologous expression of ApDnaK , the heat‐shock protein Hsp70 from the halophilic cyanobacterium Aphanothece halophytica , diminished salt‐induced injury in P. alba (Takabe et al . ). Heat shock proteins (HSPs) serve as molecular chaperones to prevent protein misfolding and aggregation under conditions of high salinity.…”

Section: Improvement Of Tree Salt Tolerance By Genetic Engineeringmentioning

confidence: 97%

On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats

Polle

Chen

2014

Plant Cell & Environment

135

114

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Saline and sodic soils that cannot be used for agriculture occur worldwide. Cultivating stress-tolerant trees to obtain biomass from salinized areas has been suggested. Various tree species of economic importance for fruit, fibre and timber production exhibit high salinity tolerance. Little is known about the mechanisms enabling tree crops to cope with high salinity for extended periods. Here, the molecular, physiological and anatomical adjustments underlying salt tolerance in glycophytic and halophytic model tree species, such as Populus euphratica in terrestrial habitats, and mangrove species along coastlines are reviewed. Key mechanisms that have been identified as mediating salt tolerance are discussed at scales from the genetic to the morphological level, including leaf succulence and structural adjustments of wood anatomy. The genetic and transcriptomic bases for physiological salt acclimation are salt sensing and signalling networks that activate target genes; the target genes keep reactive oxygen species under control, maintain the ion balance and restore water status. Evolutionary adaptation includes gene duplication in these pathways. Strategies for and limitations to tree improvement, particularly transgenic approaches for increasing salt tolerance by transforming trees with single and multiple candidate genes, are discussed.

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“…The transformation of plants with genes consequent from cyanobacteria has been significantly established since the 1990s, with genes complicated in carbon metabolism, fatty acid biosynthesis, and pigment biosynthesis (Ahmad et al 2016 ). Transformation with the gene like heat shock protein 70 (HSP70) Aphanothece halophytica rises its tolerance against high or low temperatures, drought, and salinity (Takabe et al 2008 ). Likewise, the overexpression of genes of flavodoxins from Anabaena sp.…”

Section: The Mechanistic Approach Behind the Improvement And Protecti...mentioning

confidence: 99%

Cyanobacteria as a Valuable Natural Resource for Improved Agriculture, Environment, and Plant Protection

Abo-Shady

Osman

Gaafar

et al. 2023

Water Air Soil Pollut

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Taking into consideration, the challenges faced by the environment and agro-ecosystem make increased for suggestions more reliable methods to help increase food security and deal with difficult environmental problems. Environmental factors play a critical role in the growth, development, and productivity of crop plants. Unfavorable changes in these factors, such as abiotic stresses, can result in plant growth deficiencies, yield reductions, long-lasting damage, and even death of the plants. In reflection of this, cyanobacteria are now considered important microorganisms that can improve the fertility of soils and the productivity of crop plants due to their different features like photosynthesis, great biomass yield, ability to fix the atmospheric N2, capability to grow on non-arable lands, and varied water sources. Furthermore, numerous cyanobacteria consist of biologically active substances like pigments, amino acids, polysaccharides, phytohormones, and vitamins that support plant growth enhancement. Many studies have exposed the probable role of these compounds in the alleviation of abiotic stress in crop plants and have concluded with evidence of physiological, biochemical, and molecular mechanisms that confirm that cyanobacteria can decrease the stress and induce plant growth. This review discussed the promising effects of cyanobacteria and their possible mode of action to control the growth and development of crop plants as an effective method to overcome different stresses. Graphical Abstract

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Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants

Cited by 20 publications

References 26 publications

Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants

Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants

On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats

Cyanobacteria as a Valuable Natural Resource for Improved Agriculture, Environment, and Plant Protection

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