Overexpression of gastrin-releasing peptide receptor induced layer disorganization in brain

Iwabuchi, Maiko; Maekawa, Fumihiko; Ohki‐Hamazaki, Hiroko

doi:10.1016/j.neuroscience.2005.11.014

Cited by 4 publications

(2 citation statements)

References 56 publications

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“…The first 17 amino acids of GRP was as previously mentioned the only ChrZ-derived peptide detected in our data and it was generally more highly expressed in males compared to females (in average 66%). GRP is an interesting candidate for sex specific brain development as expression of its receptor has been related to the layering of cells in the chick telencephalon, optical tectum and cerebellum [35]. The GnIH-RP2 peptide is part of the gonadotropin inhibitory hormone (GnIH) precursor protein (UniProt GNIH_COTJA) whose main peptide (GnIH) inhibits gonadotropin release in quail and is strongly expressed in all avian species studied so far [36,37].…”

Section: Discussionmentioning

confidence: 99%

Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

et al. 2010

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BackgroundEndogenous peptides such as neuropeptides are involved in numerous biological processes in the fully developed brain but very little is known about their role in brain development. Japanese quail is a commonly used bird model for studying sexual dimorphic brain development, especially adult male copulatory behavior in relation to manipulations of the embryonic endocrine system. This study uses a label-free liquid chromatography mass spectrometry approach to analyze the influence of age (embryonic days 12 vs 17), sex and embryonic day 3 ethinylestradiol exposure on the expression of multiple endogenous peptides in the developing diencephalon.ResultsWe identified a total of 65 peptides whereof 38 were sufficiently present in all groups for statistical analysis. Age was the most defining variable in the data and sex had the least impact. Most identified peptides were more highly expressed in embryonic day 17. The top candidates for EE2 exposure and sex effects were neuropeptide K (downregulated by EE2 in males and females), gastrin-releasing peptide (more highly expressed in control and EE2 exposed males) and gonadotropin-inhibiting hormone related protein 2 (more highly expressed in control males and displaying interaction effects between age and sex). We also report a new potential secretogranin-2 derived neuropeptide and previously unknown phosphorylations in the C-terminal flanking protachykinin 1 neuropeptide.ConclusionsThis study is the first larger study on endogenous peptides in the developing brain and implies a previously unknown role for a number of neuropeptides in middle to late avian embryogenesis. It demonstrates the power of label-free liquid chromatography mass spectrometry to analyze the expression of multiple endogenous peptides and the potential to detect new putative peptide candidates in a developmental model.

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Section: Discussionmentioning

confidence: 99%

Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

et al. 2010

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“…One series of sections were fixed with 4% paraformaldehyde overnight and stained with cresyl violet. Other series were fixed with the same solution for 1 h at room temperature and processed for in situ hybridization as described previously 55 . A plasmid containing a fragment of AVT gene was prepared by PCR using chicken brain cDNA as template with primers 5 0 -agc ctt ccc cga acg cat ag-3 0 and 5 0 -agc act gca gca gac acc a-3 0 .…”

Section: Methodsmentioning

confidence: 99%

A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras

et al. 2013

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Sexual differentiation leads to structural and behavioural differences between males and females. Here we investigate the intrinsic sex identity of the brain by constructing chicken chimeras in which the brain primordium is switched between male and female identities before gonadal development. We find that the female chimeras with male brains display delayed sexual maturation and irregular oviposition cycles, although their behaviour, plasma concentrations of sex steroids and luteinizing hormone levels are normal. The male chimeras with female brains show phenotypes similar to typical cocks. In the perinatal period, oestrogen concentrations in the genetically male brain are higher than those in the genetically female brain. Our study demonstrates that male brain cells retain male sex identity and do not differentiate into female cells to drive the normal oestrous cycle, even when situated in the female hormonal milieu. This is clear evidence for a sex-specific feature that develops independent of gonadal steroids.

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Neuromedin B

Ohki‐Hamazaki¹

2016

Handbook of Hormones

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Overexpression of gastrin-releasing peptide receptor induced layer disorganization in brain

Cited by 4 publications

References 56 publications

Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras

Neuromedin B

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