Overexpression of Glyoxalase III gene in transgenic sugarcane confers enhanced performance under salinity stress

Mohanan, Manoj Vadakkenchery; Anunanthini, Pushpanathan; Padmanabhan, Sarath; Sasikumar, Thelakat; Jayanarayanan, Ashwin Narayan; Dharshini, S. Akila Parvathy; Ramalingam, Sathishkumar; Ram, Bakshi; Appunu, C.

doi:10.1007/s10265-021-01300-9

Cited by 23 publications

(13 citation statements)

References 64 publications

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“…Multiple studies have demonstrated that DJ‐1 proteins from humans and Arabidopsis activate SOD to provide oxidative stress tolerance (Girotto et al, 2014; Xu et al, 2010). A significant increase in the activity of SOD was observed in both leaf and root tissues of sugarcane overexpressing the Erianthus arundinaceus GLY III gene after 15 days of drought and salinity stress (Mohanan et al, 2020, 2021). Similarly, an altered SOD activity was observed in the EcGLY‐III transgenic plants under both control and stress conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Not only SOD, an increased peroxidase and catalase activity was also observed in the transgenic lines, which ultimately reduced cellular H 2 O 2 levels (Figure 8D). A considerable enhancement in POD activity was detected in the GLYIII sugarcane transgenic plants in response to drought and salinity stress (Mohanan et al, 2020(Mohanan et al, , 2021. A previous study revealed that overexpression of yeast Hsp31 enhanced the expression of key oxidative stress genes, including catalase, peroxidase, ascorbate peroxidase, manganese superoxide dismutase, glutathione S-transferase, copper/zinc superoxide dismutase in transgenic tobacco plant using semi-quantitative expression analysis in response to H 2 O 2 (10 mM), MG (10 mM), and NaCl (300 mM) (Melvin et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

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Glyoxalase III enhances salinity tolerance through reactive oxygen species scavenging and reduced glycation

Ghosh

Mustafiz

Pareek

et al. 2022

Physiologia Plantarum

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Methylglyoxal (MG) is a metabolically generated highly cytotoxic compound that accumulates in all living organisms, from Escherichia coli to humans, under stress conditions. To detoxify MG, nature has evolved reduced glutathione (GSH)‐dependent glyoxalase and NADPH‐dependent aldo‐keto reductase systems. But both GSH and NADPH have been reported to be limiting in plants under stress conditions, and thus detoxification might not be performed efficiently. Recently, glyoxalase III (GLY III)‐like enzyme activity has been reported from various species, which can detoxify MG without any cofactor. In the present study, we have tested whether an E. coli gene, hchA, encoding a functional GLY III, could provide abiotic stress tolerance to living systems. Overexpression of this gene showed improved tolerance in E. coli and Saccharomyces cerevisiae cells against salinity, dicarbonyl, and oxidative stresses. Ectopic expression of the E. coli GLY III gene (EcGLY‐III) in transgenic tobacco plants confers tolerance against salinity at both seedling and reproductive stages as indicated by their height, weight, membrane stability index, and total yield potential. Transgenic plants showed significantly increased glyoxalase and antioxidant enzyme activity that resisted the accumulation of excess MG and reactive oxygen species (ROS) during stress. Moreover, transgenic plants showed more anti‐glycation activity to inhibit the formation of advanced glycation end product (AGE) that might prevent transgenic plants from stress‐induced senescence. Taken together, all these observations indicate that overexpression of EcGLYIII confers salinity stress tolerance in plants and should be explored further for the generation of stress‐tolerant plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Glyoxalase III enhances salinity tolerance through reactive oxygen species scavenging and reduced glycation

Ghosh

Mustafiz

Pareek

et al. 2022

Physiologia Plantarum

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“… 93 The sugarcane plants overexpressing the EaGlyIII gene showed higher levels of RWC, photosynthetic pigments, osmolytes, and ROS scavenging enzyme activities compared to the non-transformed plants under high salt stress conditions. 60 Heat shock proteins (HSPs) are a family of proteins produced by cells in response to exposure to stressful situations. Augustine et al 59 (2015) showed that the E. arundinaceus HSP70 overexpression in sugarcane plays a significant protective role in plants exposed to water deficit and salt excess.…”

Section: Tolerance To Abiotic Stressmentioning

confidence: 99%

Genetic engineering: an efficient approach to mitigating biotic and abiotic stresses in sugarcane cultivation

Song

Budeguer

Nikpay³

et al. 2022

Plant Signaling & Behavior

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Abiotic stresses are the foremost limiting factors for crop productivity. Crop plants need to cope with adverse external pressure caused by various environmental conditions with their intrinsic biological mechanisms to keep their growth, development, and productivity. Climate-resilient, high-yielding crops need to be developed to maintain sustainable food supply. Over the last decade, understanding of the genetic complexity of agronomic traits in sugarcane has prompted the integrated application of genetic engineering to address specific biological questions. Genes for adaptation to environmental stress and yield enhancement traits are being determined and introgressed to develop elite sugarcane cultivars with improved characteristics through genetic engineering approaches. Here, we discuss the advancement to provide a reference for future sugarcane ( Saccharum spp.) genetic engineering.

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“…However, plants have developed an efficient tolerance mechanism to remove an increased accumulation of toxic metabolites, namely ROS scavenging enzymes of the glyoxalase pathway [glyoxalase I (Gly I), glyoxalase II (Gly II) and glyoxalase III (Gly III)]. Mohanan et al (2021) overexpressed the EaGly III gene in sugarcane obtaining transgenic lines that exhibited significantly higher water status, gas exchange parameters, chlorophyll, carotenoid, and proline content, total soluble sugars, superoxide dismutase and peroxidase activity compared to non-transformed parental plants under salinity stress.…”

Section: Abiotic Stress Tolerancementioning

confidence: 99%

Genetic Transformation of Sugarcane, Current Status and Future Prospects

et al. 2021

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Sugarcane (Saccharum spp.) is a tropical and sub-tropical, vegetative-propagated crop that contributes to approximately 80% of the sugar and 40% of the world’s biofuel production. Modern sugarcane cultivars are highly polyploid and aneuploid hybrids with extremely large genomes (>10 Gigabases), that have originated from artificial crosses between the two species, Saccharum officinarum and S. spontaneum. The genetic complexity and low fertility of sugarcane under natural growing conditions make traditional breeding improvement extremely laborious, costly and time-consuming. This, together with its vegetative propagation, which allows for stable transfer and multiplication of transgenes, make sugarcane a good candidate for crop improvement through genetic engineering. Genetic transformation has the potential to improve economically important properties in sugarcane as well as diversify sugarcane beyond traditional applications, such as sucrose production. Traits such as herbicide, disease and insect resistance, improved tolerance to cold, salt and drought and accumulation of sugar and biomass have been some of the areas of interest as far as the application of transgenic sugarcane is concerned. Although there have been much interest in developing transgenic sugarcane there are only three officially approved varieties for commercialization, all of them expressing insect-resistance and recently released in Brazil. Since the early 1990’s, different genetic transformation systems have been successfully developed in sugarcane, including electroporation, Agrobacterium tumefaciens and biobalistics. However, genetic transformation of sugarcane is a very laborious process, which relies heavily on intensive and sophisticated tissue culture and plant generation procedures that must be optimized for each new genotype to be transformed. Therefore, it remains a great technical challenge to develop an efficient transformation protocol for any sugarcane variety that has not been previously transformed. Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. The objective of this review is to summarize current techniques and state of the art in sugarcane transformation and provide information on existing and future sugarcane improvement by genetic engineering.

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Overexpression of Glyoxalase III gene in transgenic sugarcane confers enhanced performance under salinity stress

Cited by 23 publications

References 64 publications

Glyoxalase III enhances salinity tolerance through reactive oxygen species scavenging and reduced glycation

Glyoxalase III enhances salinity tolerance through reactive oxygen species scavenging and reduced glycation

Genetic engineering: an efficient approach to mitigating biotic and abiotic stresses in sugarcane cultivation

Genetic Transformation of Sugarcane, Current Status and Future Prospects

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