Overexpression of hormone-sensitive lipase in Chinese hamster ovary cells leads to abnormalities in cholesterol homeostasis

Kraemer, Fredric B.; Fong, Loren G.; Patel, Shushma; Natu, Vanita; Komaromy, Michael

doi:10.1016/s0022-2275(20)37173-x

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…The presence of HSL in ovaries has been previously reported (Cook et al 1983; Holm et al 1987; Kraemer et al 1993; Lindvall et al 2004), and it has also been shown that overexpression of HSL in Chinese hamster ovary cells leads to abnormalities in cholesterol homeostasis (Kraemer et al 1997). However, there are no previous studies of its cellular localization and how this might change during the estrus cycle of the ovary.…”

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confidence: 81%

“…The presence of HSL in human Leydig cells suggests a role for this protein in steroidogenesis and testicular cholesterol metabolism. Furthermore, this protein seems to be involved in spermiogenesis, as suggested by its presence in spermatids (Kraemer et al 1997; Osuga et al 2000; Arenas et al 2004) and because HSL knockout mice are infertile (Osuga et al 2000).…”

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confidence: 99%

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Hormone-sensitive Lipase Expression and IHC Localization in the Rat Ovary, Oviduct, and Uterus

Lobo

Huerta

Arenas

et al. 2008

J Histochem Cytochem.

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S U M M A R Y Hormone-sensitive lipase (HSL) is a key regulator of cholesterol esters metabolism. The aim of this study was to determine HSL localization in rat female reproductive organs during the ovarian cycle by IHC methods. HSL was located in the ovarian epithelium. The granulosa cells and oocytes of primordial follicles were immunonegative. In mature follicles, HSL was found in oocytes and theca and granulosa cells. However, HSL expression in theca cells and oocytes decreased during follicular atresia. Luteal cells showed HSL staining in cytoplasm during proestrus and estrus, in the nucleus during metestrus, and in cytoplasm and the nucleus during diestrus. In the tubaric ampulla, HSL was located in the epithelial cells nuclei and in the cilia during proestrus and estrus but mainly in the nucleus during metestrus and diestrus. In the isthmus, cells showed HSL immunolabeling in the nucleus and cilia during proestrus, but only in the cilia during estrus, metestrus, and diestrus. In the uterus, HSL was found in the epithelial cells nuclei. HSL-immunoreactive bands at 84, 67, 54, and 43 kDa were found in rat female reproductive organs. HSL labeling in the nucleus of epithelial and germ cells suggests an as yet unknown function for this protein, probably related to oogenesis and cell proliferation. (J Histochem Cytochem 57:51-60, 2009)

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confidence: 81%

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confidence: 99%

Hormone-sensitive Lipase Expression and IHC Localization in the Rat Ovary, Oviduct, and Uterus

Lobo

Huerta

Arenas

et al. 2008

J Histochem Cytochem.

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“…One factor may simply relate to low abundance of the enzyme. Although hormone-sensitive lipase activity is easily detectable in mouse macrophages (42,(46)(47)(48), the activity is much lower than that seen in adipose or steroidogenic cells (49). Similarly, the abundance of hormone-sensitive lipase mRNA in mouse macrophages is low (50).…”

Section: Discussionmentioning

confidence: 98%

Cholesteryl ester hydrolysis in J774 macrophages occurs in the cytoplasm and lysosomes

Avart

Bernard

Jerome

et al. 1999

Journal of Lipid Research

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The relationship of cholesteryl ester hydrolysis to the physical state of the cholesteryl ester in J774 murine macrophages was explored in cells induced to store cholesteryl esters either in anisotropic (ordered) inclusions or isotropic (liquid) inclusions. In contrast to other cell systems, the rate of cholesteryl ester hydrolysis was faster in cells containing anisotropic inclusions than in cells containing isotropic inclusions. Two contributing factors were identified. Kinetic analyses of the rates of hydrolysis are consistent with a substrate competition by co-deposited triglyceride in cells with isotropic inclusions. In addition, hydrolysis of cholesteryl esters in cells with anisotropic droplets is mediated by both cytoplasmic and lysosomal lipolytic enzymes, as shown by using the lysosomotropic agent, chloroquine, and an inhibitor of neutral cholesteryl ester hydrolase, umbelliferyl diethylphosphate. In cells containing anisotropic inclusions, hydrolysis was partially inhibited by incubation in media containing either chloroquine or umbelliferyl diethylphosphate. Together, chloroquine and umbelliferyl diethylphosphate completely inhibited hydrolysis. However, when cells containing isotropic inclusions were incubated with umbelliferyl diethylphosphate, cholesteryl ester hydrolysis was completely inhibited, but chloroquine had no effect. Transmission electron microscopy demonstrated a primarily lysosomal location for lipid droplets in cells with anisotropic droplets and both non-lysosomal and lysosomal populations of lipid droplets in cells with isotropic droplets. These results support the conclusion that there is a lysosomal component to the hydrolysis of stored cholesteryl esters in foam cells.-

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“…Paxillin (PXN) is involved in cell migration and protein scaffold formation (Lisiak et al, 2017). Hormone‐sensitive lipolysis (HSL) is an intracellular neutral lipase that mediates the hydrolysis of triglycerides and cholesterol esters (Kraemer et al, 1997). Fatty acid transporter 4 (FATP4) is involved in fatty acid transmembrane transport and fat deposition, which plays an important role in maintaining body energy homeostasis (Song et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteome analysis reveals possible heterosis for growth, immunity and antioxidation mechanisms in Macrobrachium nipponense hybrid offspring and parent populations

Jiang

et al. 2022

Aquaculture Research

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Macrobrachium nipponense, also known as river prawn, belongs to the phylum Arthropoda, Crustacea, Decapoda, and is widely distributed in freshwater and low-salinity estuaries in Japan, Korea, Vietnam and China (Li et al., 2019). M. nipponense is an important economic species in China, which is favoured by aquaculture farmers due to their short reproduction cycle, delicious meat and rich nutritional value (Hongtuo et al., 2012). In recent years, with the gradual expansion of artificial breeding operations, issues in the culturing of M. nipponense have gradually emerged, including the degradation of shrimp germplasm resources and the decline of production, which has restricted the further development of the M. nipponense culture industry (Hongtuo et al., 2012). The deterioration of M. nipponense germplasms has caused many problems, including sexual precocity, which has resulted in the failure of individual M. nipponense to grow to the size required by commercial specifications, which has greatly affected its sales market (Bai et al., 2015). Therefore, it is necessary

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Overexpression of hormone-sensitive lipase in Chinese hamster ovary cells leads to abnormalities in cholesterol homeostasis

Cited by 9 publications

References 27 publications

Hormone-sensitive Lipase Expression and IHC Localization in the Rat Ovary, Oviduct, and Uterus

Hormone-sensitive Lipase Expression and IHC Localization in the Rat Ovary, Oviduct, and Uterus

Cholesteryl ester hydrolysis in J774 macrophages occurs in the cytoplasm and lysosomes

Comparative proteome analysis reveals possible heterosis for growth, immunity and antioxidation mechanisms in Macrobrachium nipponense hybrid offspring and parent populations

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