Overexpression of Arabidopsis thaliana tryptophan synthase beta 1 (AtTSB1) in Arabidopsis and tomato confers tolerance to cadmium stress

Sanjaya,; Hsiao, Pao-Yuan; Su, Ruey-Chih; Ko, Swee-Suak; Tong, Chii-Gong; Yang, Ray-Yu; Chan, Ming‐Tsair

doi:10.1111/j.1365-3040.2008.01819.x

Cited by 67 publications

(31 citation statements)

References 53 publications

(98 reference statements)

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“…Because Trp biosynthesis is related to Cd tolerance in plants (Sanjaya et al, 2008) and ASA2 is a key enzyme involved in Trp biosynthesis (Song et al, 1998;Tsai et al, 2005), we selected ASA2 as a potential Cd tolerance-related gene for further study.…”

Section: Identification Of Pvmtf-1 Target Genes In Tobacco By Chip-clmentioning

confidence: 99%

“…It has been shown that increasing free Trp content improves Cd tolerance in some plants such as Arabidopsis and tomato by reducing Cd accumulation (Sanjaya et al, 2008). Overexpression of ASA2 in tobacco increases free Trp content (Tsai et al, 2005).…”

Section: Pvmtf-1 Improves CD Tolerance In Tobacco By Activation Of Trmentioning

confidence: 99%

“…Recently, the Trp biosynthesis involved in Cd detoxification has emerged in plants. Increasing Trp biosynthesis in vivo or application of Trp in vitro can significantly improve Cd tolerance in Arabidopsis and tomato (Solanum lycopersicum) plants (Sanjaya et al, 2008). This is achieved by reducing Cd accumulation and enhancing antioxidant defense in plants.…”

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confidence: 99%

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Bean Metal-Responsive Element-Binding Transcription Factor Confers Cadmium Resistance in Tobacco

Sun

Zhang

et al. 2015

Plant Physiology

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Cadmium (Cd) is highly toxic to plants. Modulation of Cd-responsive transcription is an important way for Cd detoxification in plants. Metal-responsive element (MRE) is originally described in animal metallothionein genes. Although functional MREs also exist in Cd-regulated plant genes, specific transcription factors that bind MRE to regulate Cd tolerance have not been identified. Previously, we showed that Cd-inducible bean (Phaseolus vulgaris) stress-related gene2 (PvSR2) produces a short (S) PvSR2 transcript (S-PvSR2) driven by an intronic promoter. Here, we demonstrate that S-PvSR2 encodes a bean MRE-binding transcription factor1 (PvMTF-1) that confers Cd tolerance in tobacco (Nicotiana tabacum). PvMTF-1 expression was up-regulated by Cd at the levels of RNA and protein. Importantly, expression of PvMTF-1 in tobacco enhanced Cd tolerance, indicating its role in regulating Cd resistance in planta. This was achieved through direct regulation of a feedback-insensitive Anthranilate Synthase a-2 chain gene (ASA2), which catalyzes the first step for tryptophan biosynthesis. In vitro and in vivo DNA-protein interaction studies further revealed that PvMTF-1 directly binds to the MRE in the ASA2 promoter, and this binding depends on the zinc finger-like motif of PvMTF-1. Through modulating ASA2 up-regulation by Cd, PvMTF-1 increased free tryptophan level and subsequently reduced Cd accumulation, thereby enhancing Cd tolerance of transgenic tobacco plants. Consistent with this observation, tobacco transiently overexpressing ASA2 also exhibited increased tolerance to Cd. We conclude that PvMTF-1 is a zinc finger-like transcription factor that links MRE to Cd resistance in transgenic tobacco through activation of tryptophan biosynthesis.

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Section: Identification Of Pvmtf-1 Target Genes In Tobacco By Chip-clmentioning

confidence: 99%

Section: Pvmtf-1 Improves CD Tolerance In Tobacco By Activation Of Trmentioning

confidence: 99%

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Bean Metal-Responsive Element-Binding Transcription Factor Confers Cadmium Resistance in Tobacco

Sun

Zhang

et al. 2015

Plant Physiology

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“…The addition of a suitable amount of auxin under cadmium stress reduced the damage of plants due to cadmium to a certain level (Mingming et al, 2010). Sanjaya et al (2008) reported that the exogenous application of tryptophan (TRP) improves the cadmium tolerance ability of Arabidopsis and tomato plants. Keeping in view the positive role of auxin, this study was conducted to find out the best level of L-TRP to improve growth and yield of rice in cadmium-contaminated soil.…”

Section: Introductionmentioning

confidence: 99%

Auxin-mediated growth of rice in cadmium-contaminated soil

Farooq¹,

Asghar²,

Khan³

et al. 2015

Turk J Agric For

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Pollution from heavy metals is a global problem that is very dangerous to the environment. Among the heavy metals, cadmium is receiving more and more attention because it is one of the most ecotoxic, making it very harmful for biological activity in soil, biodiversity, plant metabolism, and human and animal health. Phytohormones, produced by plants, control or regulate germination, growth, metabolism, or other physiological activities, and they can alleviate the toxic effects of heavy metals on plant growth. Auxin is the most abundant natural growth hormone, controlling apical dominance, tropism, elongation of shoots, and initiation of roots in plants. However, cadmium stress causes the degradation of indigenous auxin. To cope with this, auxin may be applied exogenously. To evaluate the role of auxin in cadmium -contaminated soil, different levels of the auxin precursor L-TRP were applied to plants growing in contaminated soil. Rice seedlings were dipped in a L-TRP solution for 2 h and transplanted into potted soil contaminated with 30 mg kg -1 cadmium. The results showed that the exogenous application of L-TRP improved the growth and yield of treated plants both in normal and contaminated soil. The data of different parameters showed that L-TRP enhanced plant height (12.54%), the number of tillers (25.53%), the number of panicles (19.04%), the 1000 grain weight (19.28%), and the paddy yield (11.78%) in cadmiumcontaminated soil, as compared to plants grown in the absence of L-TRP with the same level of contamination. L-TRP increased the uptake of cadmium in rice straw while decreasing the translocation of cadmium towards grains, as compared to plants grown in contaminated soil without L-TRP. This experiment revealed the possibility of using L-TRP to improve plant growth and soil health in contaminated soil.

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“…A clorose foliar é descrita na literatura como um sintoma comum relacionado ao estresse por Cd e outros metais pesados (PILON-SMITS et al, 2000;HAJDUCH et al, 2001;SANJAYA et al, 2008;MONTEIRO et al, 2011;PIOTTO, 2012), assim como inibições de proteínas que participam dos processos de fixação de carbono e fotossíntese foram relatados em análise proteômicas de diferentes espécies (como por exemplo, as subunidades maiores e menores da RuBisCO, RuBisCO ativase, Chaperona de Cobre -CCH e fosfoglicomutase), o que pode auxiliar no entendimento da exibição desse sintoma morfológico (AHSAN et al, 2009;SEMANE et al, 2010;VILLIERS, et al 2011). …”

Section: Mt -unclassified

Análise proteômica de tomateiro (Solanum lycopersicum L.) em resposta ao cádmio

Franco¹

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AGRADECIMENTOSAgradeço primeiramente meu orientador, Prof. Ricardo, obrigado por confiar em mim, e me acolher entre seus orientados. Tenho grande orgulho de poder ter sido orientada por uma pessoa tão grandiosa, profissionalmente e pessoalmente.Agradeço à Salete Gaziola, por sempre estar do nosso lado, nos ajudando da melhor forma possível em todos os momentos necessários. Sem você, nosso lab. não serio o mesmo.Agradeço especialmente ao colega de laboratório e amigo Fernando Piotto, sem o qual não poderia ter desenvolvido esse projeto. Obrigada por seus conselhos e orientações! Agradeço aos colegas Daiana, Lucas e Luis Felipe pelas discussões sobre a técnica 2-DE, que fizeram toda a diferença! Aos meus colegas de laboratório, os quais foram muito importantes nessa caminhada, me ajudando a amadurecer de diversas formas. Meu muito obrigada a Aline, Amerivan, Berenice, Carolina, Deived, Daniel, Fabiana, Flávia, Georgia, Jackeline, Manuella, Milca, Paula, Priscila, Roberta, Rogério, Sandra, Tiago, Vanessa e Vinícius.Às amigas inestimáveis Gicka, Lucélia e Xiuá, pelo companheirismo nas horas boas e ruins, por compartilhar a "xaranguisse" de todos os dias e pelas experiências profissionais transmitidas.Ao Departamento de Genética, todos os seus professores e funcionários, agradeço pela estrutura e conhecimentos transmitidos.À ESALQ/USP, por permitir que eu também faça parte da história dessa escola centenária e de tão estimada valia para nosso país.À CNPq pela concessão de bolsa, e também a CAPES e FAPESP pelo apoio a pesquisa. RESUMO Análise proteômica de tomateiro (Solanum lycopersicum L.) em resposta ao cádmioAtualmente a contaminação ambiental é um problema mundial. A contaminação do solo por metais pesados tem aumentado nos últimos anos, estando associada à demandas de tecnologias que exigem esses elementos contaminantes. O cádmio (Cd) é um elemento normalmente encontrado em baixas concentrações na natureza, sendo altamente tóxico para microrganismos, plantas e animais. Diante disso, o presente trabalho teve como objetivo analisar as modificações no perfil protéico de duas cultivares de tomateiro, cv. Calabash Rouge (CR) e cv. Pusa Ruby (PR), submetidas a 50 µM de CdCl 2, em sistema de hidroponia. Diferentes métodos de extração de proteínas para as análises de eletroforese bidimensional (2-DE) foram conduzidas (Fenol; TCA/Acetona; Tris-base; Tris/TCA). O método TCA/Acetona foi o mais eficiente, apresentando maior rendimento total protéico (mg gMF -1 ), padronização e abundância de proteínas, e alta resolução no perfil protéico. Após a exposição das plantas ao Cd, foram realizadas análises de taxa de crescimento, índice de tolerância, quantificação do conteúdo de Cd, malondialdeído (MDA) ABSTRACT Proteomic analysis of tomato plants (Solanum lycopersicum L.) in response to cadmiumEnvironmental contamination is currently a global problem. Soil contamination by heavy metals has increased in more recent years, associated to demands of technologies that require these contaminant elements. Cadmium (Cd) is an element...

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Overexpression of Arabidopsis thaliana tryptophan synthase beta 1 (AtTSB1) in Arabidopsis and tomato confers tolerance to cadmium stress

Cited by 67 publications

References 53 publications

Bean Metal-Responsive Element-Binding Transcription Factor Confers Cadmium Resistance in Tobacco

Bean Metal-Responsive Element-Binding Transcription Factor Confers Cadmium Resistance in Tobacco

Auxin-mediated growth of rice in cadmium-contaminated soil

Análise proteômica de tomateiro (Solanum lycopersicum L.) em resposta ao cádmio

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