Overexpression of <i>ATG8</i> in Arabidopsis Stimulates Autophagic Activity and Increases Nitrogen Remobilization Efficiency and Grain Filling

Chen, Qinwu; Soulay, Fabienne; Saudemont, Baptiste; Elmayan, Taline; Marmagne, Anne; Masclaux-Daubresse, Céline

doi:10.1093/pcp/pcy214

Cited by 70 publications

(62 citation statements)

References 46 publications

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“…Expression of WRKY33 is highly induced by PQ while its transcript levels are even lower in SF-treated plants than controls ( Table 1). Overexpression of ATG8 (different genes including ATG8E) in Arabidopsis leads to an increase in the number of autophagosomes concomitant with an activated autophagy [84]. Similarly, experimental evidence was obtained to indicate that elevated expression of ATG8H increases autophagy during abiotic stress conditions [85].…”

Section: Autophagymentioning

confidence: 92%

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Omidbakhshfard

Sujeeth

Gupta

et al. 2020

IJMS

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Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.

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Section: Autophagymentioning

confidence: 92%

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Omidbakhshfard

Sujeeth

Gupta

et al. 2020

IJMS

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“…In contrast, in Arabidopsis, overexpression of ATG5 and ATG7 stimulates the lipidation of ATG8, autophagosome formation, and autophagic flux, concomitant with increased growth, seed set, and seed oil content (Minina et al, 2018). Overexpression of ATG8 in Arabidopsis not only increases autophagosome number and promotes autophagic activity, but also improves nitrogen remobilization efficiency and grain filling (Chen et al, 2019b). These results indicate that increased autophagy improves plant productivity.…”

Section: Autophagy During Growth and Developmentmentioning

confidence: 98%

“…It was shown that 15 N remobilization is decreased significantly in ATG18a RNAi, atg5, and atg9 mutants compared with wild type plants under nitrogen starvation (Guiboileau et al, 2012). Moreover, more ammonium, amino acids, and proteins accumulate in the rosette leaves of these atg mutants than in wild type (Guiboileau et al, 2013;Chen et al, 2019b). In maize, the growth rate and seed yield of the atg12 mutant is decreased significantly in low nitrogen conditions but not in sufficient nitrogen conditions.…”

Section: Autophagy Can Be Induced By Abiotic Stressmentioning

confidence: 99%

Autophagy: An Intracellular Degradation Pathway Regulating Plant Survival and Stress Response

et al. 2020

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Autophagy is an intracellular process that facilitates the bulk degradation of cytoplasmic materials by the vacuole or lysosome in eukaryotes. This conserved process is achieved through the coordination of different autophagy-related genes (ATGs). Autophagy is essential for recycling cytoplasmic material and eliminating damaged or dysfunctional cell constituents, such as proteins, aggregates or even entire organelles. Plant autophagy is necessary for maintaining cellular homeostasis under normal conditions and is upregulated during abiotic and biotic stress to prolong cell life. In this review, we present recent advances on our understanding of the molecular mechanisms of autophagy in plants and how autophagy contributes to plant development and plants' adaptation to the environment.

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“…Li et al reported that a banana MaATG8f improved drought stress tolerance in transgenic Arabidopsis through modulating reactive oxygen species (ROS) metabolism, abscisic acid biosynthesis, and autophagy activity [22]. Chen et al overexpressed an ATG8 in Arabidopsis that increased autophagic activity and improved nitrogen remobilization efficiency [23]. Moreover, overexpression of a SiATG8a of foxtail millet conferred tolerance to both nitrogen starvation and drought in transgenic Arabidopsis and rice [11,24].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Autophagy-Related Genes in Sweet Orange (Citrus sinensis) Highlights Their Roles in Response to Abiotic Stresses

Zhou

et al. 2020

IJMS

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Autophagy is a highly conserved intracellular degradation pathway that breaks down damaged macromolecules and/or organelles. It is involved in plant development and senescence, as well as in biotic and abiotic stresses. However, the autophagy process and related genes are largely unknown in citrus. In this study, we identified 35 autophagy-related genes (CsATGs—autophagy-related genes (ATGs) of Citrus sinensis, Cs) in a genome-wide manner from sweet orange (Citrus sinensis). Bioinformatic analysis showed that these CsATGs were highly similar to Arabidopsis ATGs in both sequence and phylogeny. All the CsATGs were randomly distributed on nine known (28 genes) and one unknown (7 genes) chromosomes. Ten CsATGs were predicted to be segmental duplications. Expression patterns suggested that most of the CsATG were significantly up- or down-regulated in response to drought; cold; heat; salt; mannitol; and excess manganese, copper, and cadmium stresses. In addition, two ATG18 members, CsATG18a and CsATG18b, were cloned from sweet orange and ectopically expressed in Arabidopsis. The CsATG18a and CsATG18b transgenic plants showed enhanced tolerance to osmotic stress, salt, as well as drought (CsATG18a) or cold (CsATG18b), compared to wild-type plants. These results highlight the essential roles of CsATG genes in abiotic stresses.

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Overexpression of ATG8 in Arabidopsis Stimulates Autophagic Activity and Increases Nitrogen Remobilization Efficiency and Grain Filling

Cited by 70 publications

References 46 publications

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Autophagy: An Intracellular Degradation Pathway Regulating Plant Survival and Stress Response

Comprehensive Analysis of Autophagy-Related Genes in Sweet Orange (Citrus sinensis) Highlights Their Roles in Response to Abiotic Stresses

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