Overexpression of<i>SeNHX1</i>improves both salt tolerance and disease resistance in tobacco

Chen, Xianyang; Bao, Hexigeduleng; Guo, Jie; Jia, Wenbao; Li, Yinxin

doi:10.4161/15592324.2014.993240

Cited by 16 publications

(8 citation statements)

References 12 publications

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“…The transport of Na + into vacuoles is thought to be mediated by vacuolar Na + /H + antiporters of the NHX family, which are driven by the electrochemical gradient of protons (Gaxiola et al, 1999;Pardo et al, 2006). Several studies showed that NHX1 overexpression increased salinity tolerance in plants (Apse et al, 1999;Tian et al, 2006;Brini et al, 2007;Chen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Fall¹,

Bakhoum²,

Fall³

et al. 2016

Afr. J. Biotechnol.

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Salinity is among the most widespread environmental threats to global plant production, especially in arid and semi-arid climates. Thus, the selection of salt tolerant species is necessary for sustainable plant productivity. The purpose of this study was to measure and understand the salt tolerance of three multipurpose trees used in reforestation programs in many Sahelian countries (Senegalia senegal, Syn. Acacia senegal; Vachellia seyal, Syn. A. seyal, and Prosopis juliflora). The effect of salinity was evaluated at seed germination stage on Petri dishes containing water agar (0.9%, w/v) with seven concentrations of NaCl (0, 86, 171, 257, 342, 428, and 514 mM). Our results showed that all the species had a germination rate higher than 85% at 257 mM. However, it decreased at 342 mM with a reduction of 70 and 20%, respectively for S. senegal and V. seyal. For plants growth and physiological responses, seedlings were individually cultivated in plastic bags (25×12 cm) containing non-sterile soil and watered with four salt solutions (0, 86, 171 and 257 mM NaCl). Four months after the plants' cultivation, the results showed that for all species, the salinity reduced significantly the height, the collar diameter, the shoot and root dry biomass as well as the total chlorophyll, K + and K + /Na + ratio. In the meantime, proline content, Cland Na + accumulation in leaves were increased. It was also found that S. senegal and V. seyal tolerated high concentrations of NaCl (257 mM) and developed physiological and molecular mechanisms, such as salt tolerance genes (NHX1), which allow them to be considered as moderated salt tolerant species and seemed to be potential species for the restoration of salt-affected land as P. juliflora.

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Section: Introductionmentioning

confidence: 99%

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Fall¹,

Bakhoum²,

Fall³

et al. 2016

Afr. J. Biotechnol.

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“…However, previous studies in various plant species have demonstrated that overexpressing the single gene, vacuolar Na + (K + )/H + antiporter gene, is a feasible way to create transgenic plants with enhanced salt tolerance (Mishra et al, 2014; Chen et al, 2015; Zhang et al, 2015; Sahoo et al, 2016). Consistent with these studies, transgenic switchgrass overexpressing PvNHX1 exhibited better growth performance (Figure 5) and maintained an improved physiological capacity (Figures 6, Figure S4) comparing to control plants under high salinity stress.…”

Section: Discussionmentioning

confidence: 99%

“…NHXs are ubiquitous to all eukaryotic organisms and implicate a pivotal function in regulating plant responses to salt stress (Zhang et al, 2015; Sahoo et al, 2016), cold tolerance (Li et al, 2007), drought tolerance (Wang et al, 2016) and disease resistance (Chen et al, 2015). The successful expressions of the key enzyme genes related to the NHXs were proved to be valid to enhance the capacity for salt tolerance in diverse species, such as and cowpea (Mishra et al, 2014), tobacco (Chen et al, 2015), alfalfa (Zhang et al, 2015), and mungbean (Sahoo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The successful expressions of the key enzyme genes related to the NHXs were proved to be valid to enhance the capacity for salt tolerance in diverse species, such as and cowpea (Mishra et al, 2014), tobacco (Chen et al, 2015), alfalfa (Zhang et al, 2015), and mungbean (Sahoo et al, 2016). In spite of numerous work has focused on use of NHXs in salt tolerance, the mechanism underlying the enhancement of salinity tolerance by NHXs remained unclear (Sahoo et al, 2016; Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

et al. 2017

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Switchgrass (Panicum virgatum L.) has been increasingly recognized as one of the most valuable perennial bioenergy crop. To improve its biomass production, especially under salt stress, we isolated a putative vacuolar Na+ (K+)/H+ antiporter gene from switchgrass and designated as PvNHX1. Subcellular localization revealed that this protein was localized mainly on the vacuole membrane. The PvNHX1 was found to be expressed throughout the entire growth period of switchgrass, exhibited preferentially expressed in the leaf tissue, and highly induced by salt stress. Transgenic switchgrass overexpressing PvNHX1 showed obvious advantages with respect to plant height and leaf development compared to the wild-type (WT) and transgenic control (EV, expressing the empty vector only) plants, suggesting PvNHX1 may serve as a promoter in switchgrass growth and development. Moreover, transgenic switchgrass were more tolerant than control plants with better growth-related phenotypes (higher shoot height, larger stem diameter, longer leaf length, and width) and physiological capacities (increased proline accumulation, reduced malondialdehyde production, preserved cell membrane integrity, etc.) under high salinity stress. Furthermore, the genes related to cell growth, flowering, and potassium transporters in transgenic switchgrass exhibited a different expression profiles when compared to the control plants, indicating a pivotal function of PvNHX1 in cell expansion and K+ homeostasis. Taken together, PvNHX1 is essential for normal plant growth and development, and play an important role in the response to salt stress by improving K+ accumulation. Our data provide a valuable foundation for further researches on the molecular mechanism and physiological roles of NHXs in plants.

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“…NbNHX1 silencing led to a lower pH in the vacuole and a lower cellular ROS level in N. benthamiana, which was coupled with a decreased NAD(P) (H) pool and decreased expression of ROSresponsive genes [43]. Overexpression of SeNHX1 intensified the compartmentation of Na + into vacuole under salt stress and improved the ability of eliminating ROS after pathogen attack, which then enhanced salt tolerance and disease resistance simultaneously in tobacco [44]. SeNHX1, AtNHX1, sbNHX1 and NbNHX1 transgenic tobaccos exhibited more biomass, longer root length, and higher Na+/H+ ratio under NaCl treatment, indicating enhanced salt tolerance [45].…”

Section: Ion Transport Genes Related To Tobacco Salt Tolerancementioning

confidence: 99%

Advances in salt tolerance molecular mechanism in tobacco plants

Sun

Wang

2020

Hereditas

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Tobacco, an economic crop and important model plant, has received more progress in salt tolerance with the aid of transgenic technique. Salt stress has become a key research field in abiotic stress. The study of tobacco promotes the understanding about the important adjustment for survival in high salinity environments, including cellular ion transport, osmotic regulation, antioxidation, signal transduction and expression regulation, and protection of cells from stress damage. Genes, which response to salt, have been studied using targeted transgenic technologies in tobacco plants to investigate the molecular mechanisms. The transgenic tobacco plants exhibited higher seed germination and survival rates, better root and shoot growth under salt stress treatments. Transgenic approach could be the promising option for enhancing tobacco production under saline condition. This review highlighted the salt tolerance molecular mechanisms of tobacco.

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Overexpression ofSeNHX1improves both salt tolerance and disease resistance in tobacco

Cited by 16 publications

References 12 publications

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

Advances in salt tolerance molecular mechanism in tobacco plants

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