Overexpression of ICE1 gene in mungbean (Vigna radiata L.) for cold tolerance

Rout, Gyana Ranjan; Bansal, Anuradha; Swain, D.; Jadhao, Kundansing Rajpalsing; Shelke, Rahul G.; Panda, Sanjib Kumar

doi:10.1007/s11240-020-01944-w

Cited by 15 publications

(7 citation statements)

References 52 publications

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“…On sub-culturing on SM, proliferation of multiple shoots and an average of 3-4 shoots were observed in all varieties (Fig. 4d) These results were consistent with the findings of previous reports in mungbean (Avenido and Hattori, 2001;Mundhara and Rashid, 2006;Yadav et al, 2010;Rout et al, 2020). During shoot proliferation many number of plantlets were lost due to contamination and rest of plantlets which survived (7 in number: KKM-7-MAC-14, KKM-8-MAC-14, IC-1-MAC-14, KKM-2-MAC-19, LM-8-MAC-19, CM-11-MAC-17 and CM-4-MAC-19), were transferred to SEM, The multiple shoots were elongated on SEM and elongation of shoots was observed.…”

Section: Development Of Transgenic Mungbean With Mymv Rep Genesupporting

confidence: 92%

Isolation, Cloning and Agrobacterium Mediated Transformation of Mymv Replicase (Rep) Gene in Vigna Radiata (L.) Wilczek for Mymv Resistance

RAMACHANDRA¹,

ANANTAPUR²,

ROSHNI³

et al. 2022

AJMBES

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The binary plant cloning vector pCAMBIA1305.2 carrying the MYMV Replicase (Rep) gene was constructed and transformed into a competent Agrobacterium tumefaciens, strain LBA4404 and was used for co-cultivation of mungbean varieties, KKM-3, IC-39340-1, China mung and LM-1668 for imparting mungbean yellow mosaic virus (MYMV) resistance. The putative transformants (To generation) were selected on shooting media (SM) supplemented with 5 mgl-1 BAP, 50 mgl-1 hygromycin and 250 mgl-1 cefotaxime. The multiple shoots were inoculated on shoot elongation media (SEM) with 0.3 mgl-1 zeatin and 2.5 mgl-1 BAP for elongation and rooted on rooting media (RM), comprising half MS supplemented with 0.2 mgl-1 NAA. The GUS assay and genomic PCR analysis of the To transformants revealed, four positive putative transgenic lines for Rep gene specific primers and had a positive correlation with vector specific hptII primers. Likewise, VirG amplification revealed no Agrobacterium contamination in the apoplast of allthe putative To transformants.

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Section: Development Of Transgenic Mungbean With Mymv Rep Genesupporting

confidence: 92%

Isolation, Cloning and Agrobacterium Mediated Transformation of Mymv Replicase (Rep) Gene in Vigna Radiata (L.) Wilczek for Mymv Resistance

RAMACHANDRA¹,

ANANTAPUR²,

ROSHNI³

et al. 2022

AJMBES

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“…Genetic transformation approaches have been applied in mungbean to identify and evaluate genes associated with adaptation to salinity (Baloda et al, 2017; Kumar et al, 2017; Sahoo et al, 2016) and cold stress (Rout et al, 2020). The approach of Kumar et al (2017) and Sahoo et al (2016) of overexpressing a gene originally found in Arabidopsis in mungbean highlights the importance of using knowledge from model species to accelerate mungbean improvement.…”

Section: Breeding For Adaptation To Abiotic Stress In Mungbeanmentioning

confidence: 99%

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Haeften

Dudley

Kang

et al. 2023

Food and Energy Security

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Mungbean (Vigna radiata (L.) R. Wilczek var. radiata) is a significant food and cash crop grown in tropical and subtropical regions. Mungbean production and consumer demand have increased substantially over the last two decades, owing to its agronomic, nutritional and economic benefits. Despite increased breeding efforts and the expansion of mungbean production in various agro‐climatic regions, further production is hindered by low yield and variability, which is partly attributed to the impacts of abiotic stress. Abiotic stress impacts on the physiology, morphology and reproductive ability of mungbean which influences yield. Exposure to abiotic stresses at the reproductive stage is considered the most critical for yield production. In this review, we evaluate how abiotic stress impacts mungbean growth and productivity when occurring during the reproductive stage and traits that may confer adaptation. We present the limitations of current research including limited number of genotypes, lack of field experiments and detailed experimental information. We highlight the opportunities to exploit new tools and technologies, such as high‐throughput phenotyping platforms, gene editing, and genomic selection, to accelerate breeding efforts to develop more resilient mungbean cultivars for today and tomorrow.

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“…In this study, EREBP1 , ICE1 and some stress responsive genes were lowly expressed in AL. ICE1 is an upstream regulator of cold-responsive genes in most plants [ 35 , 36 , 37 ]. Ectopic expression of AtICE1 and OsICE1 can delay stress-induced senescence and improve tolerance to abiotic stresses in tobacco [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Physiology and Transcriptome Analysis Provides Insights into the Regulatory Mechanism of Albinotic Bambusa oldhamii

Qian,

Ye,

et al. 2023

Plants

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Albinism is a unique problem encountered in tissue culture experiments, but the underlying mechanism remains unclear in most bamboo species. In this study, we identified the putative regulatory genes in an albino mutant of Bambusa oldhamii using comparative physiology and transcriptome analysis. The degeneration of chloroplasts, low chlorophyll (Chl) content and reduced photosynthetic capacity were observed in albinotic B. oldhamii compared to normal lines. A total of 6191 unigenes were identified that were clearly differentially expressed between albino and normal lines by transcriptome sequencing. Most genes related to chloroplast development (such as Psa, Psb) and pigment biosynthesis (such as LHC, GUN4, ZEP) were downregulated significantly in albinotic lines, which might be responsible for the albino phenotype. Moreover, some transcription factors (TFs) such as PIF and GLK1 were identified to be involved in chloroplast development and Chl synthesis, indicating the involvement of putative regulatory pathways PIF-LHC and GLK1-LHC/Psa/Psb in albinotic B. oldhamii. Finally, the downregulation of some stress responsive TFs (like ICE1 and EREB1) suggested a reduction in stress resistance of albinotic B. oldhamii. The above findings provided new insights into the molecular mechanism of albinism in bamboo.

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Overexpression of ICE1 gene in mungbean (Vigna radiata L.) for cold tolerance

Cited by 15 publications

References 52 publications

Isolation, Cloning and Agrobacterium Mediated Transformation of Mymv Replicase (Rep) Gene in Vigna Radiata (L.) Wilczek for Mymv Resistance

Isolation, Cloning and Agrobacterium Mediated Transformation of Mymv Replicase (Rep) Gene in Vigna Radiata (L.) Wilczek for Mymv Resistance

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Comparative Physiology and Transcriptome Analysis Provides Insights into the Regulatory Mechanism of Albinotic Bambusa oldhamii

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