Overexpression of integrin αv in the human nasopharyngeal carcinoma associated with metastasis and progression

Xuan, Shihai; Zhou, Yue; Pan, Ji-Qun; Zhu, Wei; Xu, Ping

doi:10.3233/cbm-130361

Cited by 13 publications

(9 citation statements)

References 18 publications

(22 reference statements)

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“…Previous studies have identified many different genes that are involved in metastasis of NPC, accelerating or suppressing metastasis by a mechanism of cooperative or inhibitive interactions between genes, such as LMP1 [18], NGX6 [19]–[20], Fibulin-5 [21], IntergrinαV[22], MIF and DJ-1[23] and so on. Our previous studies also confirmed that high expression of p-eIF4E and p-Mnk1 played a critical role in promoting invasion and metastasis and related with the poor prognosis of NPC patients [24].…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Flotillin-2 Protein as a Novel Biomarker for Lymph Node Metastasis in Nasopharyngeal Carcinoma

Wen

Wang

et al. 2014

PLoS ONE

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Nasopharyngeal carcinoma (NPC) is a head and neck malignant tumor rare throughout most of the world but common in Southeast Asia, especially in Southern China. Flotillin-2 (Flot-2) is not only an important component of cellular membrane, but also involves in various cellular processes such as membrane trafficking, T cell and B cell activation, regulation of several signaling pathways associated with cell growth and malignant transformation, keeping structure and junction of epidermal cells and formation of filopodia. Although such molecular effects of Flot-2 have been reported, whether the expression of Flot-2 protein is associated with clinicopathologic implication for NPC has not been reported. The purpose of this research is to investigate the expression of Flot-2 protein in NPC and control nasopharyngeal epithelial tissues by immunohistochemistry and elucidate the association between the expression of Flot-2 protein and clinicopathological characteristics of NPC. The results showed that the positive percentage of Flot-2 expression in the NPC, nasopharyngeal epithelia with atypical hyperplasia and in the control nasopharyngeal mucosa epithelia was 88.8% (119/134), 76.9% (10/13) and 5.7% (5/88), respectively. There was significantly higher expression of Flot-2 protein in NPC and nasopharyngeal epithelia with atypical hyperplasia compared to the control nasopharyngeal mucosa epithelia (P<0.001, respectively). The positive percentage of Flot-2 protein expression in NPC patients with lymph node metastasis was significantly higher than those without lymph node metastasis. Increasing of Flot-2 expression was obviously correlated with clinical stages of NPC patients. The expression of Flot-2 was proved to be the independent predicted factor for lymph node metastasis by multivariate analysis. The sensitivity of Flot-2 for predicting lymph node metastasis of NPC patients was 93%. Taken together, our results suggest that the increased expression of Flot-2 protein is a novel higher sensitivity biomarker that can predict lymph node metastases in NPC.

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Section: Discussionmentioning

confidence: 99%

Increased Expression of Flotillin-2 Protein as a Novel Biomarker for Lymph Node Metastasis in Nasopharyngeal Carcinoma

Wen

Wang

et al. 2014

PLoS ONE

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“…Additionally, Nakada et al [23] reported that ITGα3 is over-expressed in glioma stem-like cells and promotes invasion. Furthermore, evidence shows that overexpression of ITGαv is closely associated with the metastasis and progression of some cancers [24, 25]. …”

Section: Discussionmentioning

confidence: 99%

CLIC1 Promotes the Progression of Gastric Cancer by Regulating the MAPK/AKT Pathways

Mao

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Chloride intracellular channel 1 (CLIC1), which is a member of the chloride channel protein family, is associated with various human tumors. Recent studies have shown that CLIC1 is involved in the occurrence and development of gastric cancer (GC). However, the exact mechanism remains unclear in GC. Methods: Effects of CLIC1 on the progression of GC in vivo and in vitro and the potential underlying mechanisms have been investigated by analysing 54 patients with GC, as well as human gastric cell lines SGC-7901 and MGC-803, utilizing proteomics, RT-PCR, Western blotting, flow cytometry, Cell invasion and migration assays and xenograft tumor models. Results: Our study shows that CLIC1 knockdown by targeted-siRNA markedly inhibits GC cell invasion and migration and induces apoptosis in vitro. In total, 54 differentially expressed proteins were identified in GC cells SGC-7901 after CLIC1 silencing by isobaric tags for relative isotope labeled and absolute quantitation (iTRAQ) technology, including integrin α1 (ITGα1) and ITGα3. The expression levels of ITGα3, ITGαv, ITGβ1 and Bcl-2 mRNA and protein were decreased significantly in GC cells after CLIC1 knockdown; ITGα1 and Fas were upregulated, but the level of survivin was not significantly different. GC growth and metabolism were decreased in vivo after CLIC1 silencing, but apoptosis was markedly increased. Further study showed that the expression levels of ITGα3, ITGαv and ITGβ1, as well as AKT-phosphorylation, ERK-phosphorylation and p38-phosphorylation, were reduced in vivo after CLIC1 knockdown, while ITGα1 was upregulated. Conclusions: We speculate that CLIC1 may play an important role in the progression of GC, and its mechanism may be related to the regulation of integrin family proteins, which leads to the sequential regulation of the PI3K/AKT, MAPK/ERK and MAPK/p38 pathways.

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“…The expression of αv integrin subunits is correlated with lymphatic metastasis of laryngeal and hypopharyngeal carcinoma, although the associated integrin β subunit that plays the major role in SCC aggressiveness was not identified [53]. In nasopharyngeal carcinoma, expression levels of integrin αv mRNA and protein are also correlated with tumour size and lymph node spread, and were significantly higher in nasopharyngeal carcinoma tissues than in non-neoplastic inflammation tissues from the same region [54]. …”

Section: Integrins In Hnsccmentioning

confidence: 99%

“…Since expression of the αv and β1 subunits have been associated with proliferation [53] and metastasis [51,53,54,95,107], αvβ3 [65] and αvβ6 [80,84] associated with proliferation and invasion, targeting one or more of these receptors appears to be an attractive strategy to reduce either tumour growth or dissemination. With the exception of cilengitide, very limited preclinical studies have taken place so far [80,88,107].…”

Section: Applications Of Integrin-targeted Agentsmentioning

confidence: 99%

RGD-Binding Integrins in Head and Neck Cancers

Ahmedah

Patterson

Shnyder

et al. 2017

Cancers

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Alterations in integrin expression and function promote tumour growth, invasion, metastasis and neoangiogenesis. Head and neck cancers are highly vascular tumours with a tendency to metastasise. They express a wide range of integrin receptors. Expression of the αv and β1 subunits has been explored relatively extensively and linked to tumour progression and metastasis. Individual receptors αvβ3 and αvβ5 have proved popular targets for diagnostic and therapeutic agents but lesser studied receptors, such as αvβ6, αvβ8, and β1 subfamily members, also show promise. This review presents the current knowledge of integrin expression and function in squamous cell carcinoma of the head and neck (HNSCC), with a particular focus on the arginine-glycine-aspartate (RGD)-binding integrins, in order to highlight the potential of integrins as targets for personalised tumour-specific identification and therapy.

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Overexpression of integrin αv in the human nasopharyngeal carcinoma associated with metastasis and progression

Abstract: All these findings suggest that overexpression of integrin αv is closely associated with metastasis and progression of NPC. Therefore, we can speculate that integrin αv could be effective prognostic markers in the future for individualized treatment of patients with NPC.

Cited by 13 publications

References 18 publications

Increased Expression of Flotillin-2 Protein as a Novel Biomarker for Lymph Node Metastasis in Nasopharyngeal Carcinoma

Increased Expression of Flotillin-2 Protein as a Novel Biomarker for Lymph Node Metastasis in Nasopharyngeal Carcinoma

CLIC1 Promotes the Progression of Gastric Cancer by Regulating the MAPK/AKT Pathways

RGD-Binding Integrins in Head and Neck Cancers

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