Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila

Zhang, Gaoqun; Hao, Yangguang; Jin, Li

doi:10.1016/j.ibmb.2016.08.002

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…Similarly, more than 60% of lymph gland of the Srp> jumu RNAi lymph gland also showed obvious increased numbers of lamellocytes (n = 36) (Figure 9F,G). Our previous study demonstrated that the overexpression of jumu in both hemocytes and the fat body induces the deposition of hemocytes and melanotic nodules through the activation of Toll signaling (Zhang et al, 2016). The constitutive activation of Toll signaling in hemocytes or the fat body can lead to the differentiation of lamellocytes (Schmid et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Our results suggest that the loss of jumu in the entire lymph gland induces lamellocyte generation through the activation of Toll signaling (Figure 10B). Our previous study has showed that the overexpression of jumu in both circulating hemocytes and the fat body activates the Toll pathway in hemocytes that were deposited on the fat body, and subsequently leads to the formation of melanotic nodules (Zhang et al, 2016). These results suggest that the correct genetic dose of jumu is important for the activation of Toll-dependent immune response and chronic inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous study demonstrated that the loss of jumu affects the number and phagocytosis of circulating hemocytes (Jin et al, 2008). Moreover, the overexpression of jumu induces melanotic nodules by activating Toll signaling (Zhang et al, 2016). In this paper, we show that Jumu is expressed in the entire lymph gland and plays crucial roles during Drosophila hematopoiesis.…”

Section: Introductionmentioning

confidence: 99%

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Dual role for Jumu in the control of hematopoietic progenitors in the Drosophila lymph gland

Hao

Jin

2017

eLife

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The Drosophila lymph gland is a hematopoietic organ in which the maintenance of hematopoietic progenitor cell fate relies on intrinsic factors and extensive interaction with cells within a microenvironment. The posterior signaling center (PSC) is required for maintaining the balance between progenitors and their differentiation into mature hemocytes. Moreover, some factors from the progenitors cell-autonomously control blood cell differentiation. Here, we show that Jumeau (Jumu), a member of the forkhead (Fkh) transcription factor family, controls hemocyte differentiation of lymph gland through multiple regulatory mechanisms. Jumu maintains the proper differentiation of prohemocytes by cell-autonomously regulating the expression of Col in medullary zone and by non-cell-autonomously preventing the generation of expanded PSC cells. Jumu can also cell-autonomously control the proliferation of PSC cells through positive regulation of dMyc expression. We also show that a deficiency of jumu throughout the lymph gland can induce the differentiation of lamellocytes via activating Toll signaling.DOI: http://dx.doi.org/10.7554/eLife.25094.001

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dual role for Jumu in the control of hematopoietic progenitors in the Drosophila lymph gland

Hao

Jin

2017

eLife

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“…Phalloidin staining was performed as previously described (13). The fat body was dissected and fixed for 30 min with 4% paraformaldehyde in PBS at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Flos�Chrysanthemi�Indici extract improves a high‑sucrose diet‑induced metabolic disorder in Drosophila

Bai

Shao

et al. 2018

Exp Ther Med

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Flos Chrysanthemi Indici (CI) is a traditional medicinal plant used in the treatment of inflammatory diseases. However, the pharmacological role of CI in metabolic diseases, especially in diseases induced by insulin metabolism disorders, remains poorly understood. In the present study, Drosophila melanogaster (Drosophila) were fed with high-sugar diet (HSD) to induce a model similar to Type 2 diabetes (T2D) in order to determine whether CI extracts improve the metabolic disorder. It was demonstrated that the CI extracts could improve growth rate, body size, lifespan, reproductive capacity and fat storage, and CI especially improved the fat metabolism and cell size in S6k and Akt1 mutant flies. In conclusion, the present study provides novel evidence that CI may be an effective drug for the treatment of T2D.

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“…A recent study indicated that Jumu regulates cardiac progenitor specification by controlling the expression of receptors of the fibroblast growth factor and Wnt signaling pathways [ 31 ]. Additionally, our previous studies have shown that Jumu is also expressed in hemocytes and fat body, involved in proper bacterial phagocytosis and resistance in adult flies, and overexpression of jumu both in the fat body and hemocytes induces melanotic nodules by activating Toll signaling [ 32 , 33 ]. Our recent study showed that Jumu plays crucial roles during Drosophila lymph gland hematopoiesis [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila

Hao

Luo

et al. 2018

Cell Commun Signal

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BackgroundThe regulatory mechanisms of hematopoiesis and cellular immunity show a high degree of similarity between insects and mammals, and Drosophila has become a good model for investigating cellular immune responses. Jumeau (Jumu) is a member of the winged-helix/forkhead (FKH) transcription factor family and is required for Drosophila development. Adult jumu mutant flies show defective hemocyte phagocytosis and a weaker defense capability against pathogen infection. Here, we further investigated the role of jumu in the regulation of larval hemocyte development and phagocytosis.MethodsIn vivo phagocytosis assays, immunohistochemistry, Real-time quantitative PCR and immunoblotting were performed to investigate the effect of Jumu on hemocyte phagocytosis. 5-Bromo-2-deoxyUridine (BrdU) labeling, phospho-histone H3 (PH3) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining were performed to analyze the proliferation and apoptosis of hemocyte; immunohistochemistry and Mosaic analysis with a repressible cell marker (MARCM) clone analysis were performed to investigate the role of Jumu in the activation of Toll pathway.ResultsJumu indirectly controls hemocyte phagocytosis by regulating the expression of NimC1 and cytoskeleton reorganization. The loss of jumu also causes abnormal proliferation and differentiation in circulating hemocytes. Our results suggest that a severe deficiency of jumu leads to the generation of enlarged multinucleate hemocytes by affecting the normal cell mitosis process and induces numerous lamellocytes by activating the Toll pathway.ConclusionsJumu regulates circulating hemocyte differentiation and phagocytosis in Drosophila. Our findings provide new insight into the mechanistic roles of cytoskeleton regulatory proteins in phagocytosis and establish a basis for further analyses of the regulatory mechanism of the mammalian ortholog of Jumu in mammalian innate immunity.Electronic supplementary materialThe online version of this article (10.1186/s12964-018-0305-3) contains supplementary material, which is available to authorized users.

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Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila

Cited by 8 publications

References 37 publications

Dual role for Jumu in the control of hematopoietic progenitors in the Drosophila lymph gland

Dual role for Jumu in the control of hematopoietic progenitors in the Drosophila lymph gland

Flos�Chrysanthemi�Indici extract improves a high‑sucrose diet‑induced metabolic disorder in Drosophila

Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila

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