Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance

Jia, Fengjuan; Qi, Shengdong; Li, Hui; Liu, Pu; Li, Pengcheng; Wu, Changai; Zheng, Chengchao; Huang, Jinguang

doi:10.1016/j.bbrc.2014.10.136

Cited by 85 publications

(55 citation statements)

References 28 publications

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“…It has been reported that LEA proteins play pivotal roles in stress tolerance, as osmotic adjustment material and a protection material for cell membrane structure [44,45]. The LEA genes have been shown to enhance plant tolerance to salt stress [3,5].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco

Dang

et al. 2020

IJMS

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In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators that regulate developmental process and responses to phytohormones and stress treatments. However, the regulatory functions of the Vitis vinifera L. (grapevine) Aux/IAA transcription factor gene VvIAA18 have not been reported. In this study, the VvIAA18 gene was successfully cloned from grapevine. Subcellular localization analysis in onion epidermal cells indicated that VvIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length of VvIAA18 exhibited transcriptional activation. Salt tolerance in transgenic tobacco plants and Escherichia. coli was significantly enhanced by VvIAA18 overexpression. Real-time quantitative PCR analysis showed that overexpression of VvIAA18 up-regulated the salt stress-responsive genes, including pyrroline-5-carboxylate synthase (NtP5CS), late embryogenesis abundant protein (NtLEA5), superoxide dismutase (NtSOD), and peroxidase (NtPOD) genes, under salt stress. Enzymatic analyses found that the transgenic plants had higher SOD and POD activities under salt stress. Meanwhile, component analysis showed that the content of proline in transgenic plants increased significantly, while the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) decreased significantly. Based on the above results, the VvIAA18 gene is related to improving the salt tolerance of transgenic tobacco plants. The VvIAA18 gene has the potential to be applied to enhance plant tolerance to abiotic stress.

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Section: Discussionmentioning

confidence: 99%

Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco

Dang

et al. 2020

IJMS

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“…In Arabidopsis, 51 LEA proteins have been identified that belong to nine different groups [60]. Jia et al [61] showed overexpression of AtLEA14, which belongs to the LEA group 2 proteins, overactivates salt-stress-inducible genes such as RD29B, which encode dehydration protective proteins, and subsequently confers salt tolerance in Arabidopsis.…”

Section: Aba and Salt Tolerancementioning

confidence: 99%

Role of ABA in Arabidopsis Salt, Drought, and Desiccation Tolerance

Fernando¹,

Schroeder²

2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

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The ability of plants to respond to environmental stimuli is essential to plant survival. Abscisic acid ABA is a phytohormone with roles at various stages of plant development. ABA also plays a major role in mediating physiological responses to environmental stresses such as salt, osmotic, and cold stress. Plant responses to environmental stress have been widely studied in the model plant Arabidopsis thaliana and ABA signaling mechanisms elucidated. In general, the adaptive responses of plants to various stress conditions can be either ABA-dependent or ABA-independent. Here we focus on the role of ABA in stress signaling and abiotic stress tolerance. We describe the intrinsic mechanisms that confer stress tolerance via ABA, as well as how ABA-regulated gene products play a role in salt and drought tolerance at different stages of the life cycle. In addition, the contribution of ABA to regulation of stomatal aperture and therefore desiccation tolerance will be discussed. Understanding ABA signaling mechanisms in abiotic stress provides avenues for improving plant performance.

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“…Similar behavior was observed for their Arabidopsis homologs in ABA-insensitive1 (abi1) and abi5 and growth insensitive to ABA3 mutants (LopezMolina and Chua, 2000;Lopez-Molina et al, 2002;Kinoshita et al, 2010). Importantly, in Arabidopsis, these genes confer salt tolerance during germination (Jia et al, 2014) and acquisition of desiccation tolerance during seed maturation (Manfre et al, 2009). Decreased levels of these proteins in slggb1 seeds probably contributed to the increased germination rates observed in the presence of external ABA.…”

Section: Slggb1 Attenuates Auxin Responses During Lateral Root Formatmentioning

confidence: 59%

Type B Heterotrimeric G Protein γ-Subunit Regulates Auxin and ABA Signaling in Tomato

Subramaniam

Trusov²,

Encina³

et al. 2015

Plant Physiol.

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Heterotrimeric G proteins composed of a, b, and g subunits are central signal transducers mediating the cellular response to multiple stimuli in most eukaryotes. Gg subunits provide proper cellular localization and functional specificity to the heterotrimer complex. Plant Gg subunits, divided into three structurally distinct types, are more diverse than their animal counterparts. Type B Gg subunits, lacking a carboxyl-terminal isoprenylation motif, are found only in flowering plants. We present the functional characterization of type B Gg subunit (SlGGB1) in tomato (Solanum lycopersicum). We show that SlGGB1 is the most abundant Gg subunit in tomato and strongly interacts with the Gb subunit. Importantly, the green fluorescent proteinSlGGB1 fusion protein as well as the carboxyl-terminal yellow fluorescent protein-SlGGB1/amino-terminal yellow fluorescent protein-Gb heterodimer were localized in the plasma membrane, nucleus, and cytoplasm. RNA interference-mediated silencing of SlGGB1 resulted in smaller seeds, higher number of lateral roots, and pointy fruits. The silenced lines were hypersensitive to exogenous auxin, while levels of endogenous auxins were lower or similar to those of the wild type. SlGGB1-silenced plants also showed strong hyposensitivity to abscisic acid (ABA) during seed germination but not in other related assays. Transcriptome analysis of the transgenic seeds revealed abnormal expression of genes involved in ABA sensing, signaling, and response. We conclude that the type B Gg subunit SlGGB1 mediates auxin and ABA signaling in tomato.

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Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance

Cited by 85 publications

References 28 publications

Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco

Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco

Role of ABA in Arabidopsis Salt, Drought, and Desiccation Tolerance

Type B Heterotrimeric G Protein γ-Subunit Regulates Auxin and ABA Signaling in Tomato

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