Overexpression of lily HsfA3s in Arabidopsis confers increased thermotolerance and salt sensitivity via alterations in proline catabolism

Wu, Ze; Liang, Jiahui; Wang, Chengpeng; Zhao, Xin; Zhong, X.P.; Cao, Xinmin; Li, Guoqing; He, Junna; Yi, Mingfang

doi:10.1093/jxb/ery035

Cited by 69 publications

(46 citation statements)

References 74 publications

(100 reference statements)

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“…These data collectively suggest a role for CaHSL1 as a positive regulator in pepper basal and acquired thermotolerance under high humidity. Similarly, previous studies have found that some regulatory proteins in HSR signaling, for example, AtHsfA3, Aldehyde dehydrogenases (ALDH) (Zhao et al, 2017), ATPase6 (ALA6) (Niu et al, 2017) HsfA2 (Ogawa et al, 2007) in Arabidopsis and LlHsfA3A in lily ( Lilium longiflorum ) (Wu et al, 2018), FaHsfA2c in Festuca arundinacea (Wang et al, 2017) act as positive regulator in both basal and acquired thermotolerance, while LlHsfA3B (Wu et al, 2018) in lily acts as positive regulator only in acquired thermotolerance, salicylic acid dependent signaling (Clarke et al, 2004) promotes only basal thermotolerance, indicating that basal and acquired thermotolerance might be modulated by different signaling pathways with extensive crosstalks between them. Noticeably, our data showed that CaHSP24 and CaHSP24.2 that might localize to chloroplast (Heckathorn et al, 1996) were dramatically downregulated by silencing of CaHSL1 , coupled with significant decrease in Fv/Fm, whereas the transient overexpression of CaHSL1 significantly enhanced transcription of CaHSP24 and CaHSP24.2 as well as enhanced Fv/Fm, it can be speculated that as an indicator of plant thermotolerance, the stability of photochemical efficiency of PSII (Fv/Fm) might be contributed by multiple HSPs including CaHSP24 and CaHSP24.2 , as Fv/Fm differed in timing and amplitudes with CaHSP24 and CaHSP24.2 during response CaHSL1 silenced pepper plant to heat stress.…”

Section: Discussionsupporting

confidence: 52%

CaHSL1 Acts as a Positive Regulator of Pepper Thermotolerance Under High Humidity and Is Transcriptionally Modulated by CaWRKY40

et al. 2018

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Pepper (Capsicum annuum) is an economically important vegetable and heat stress can severely impair pepper growth, development, and productivity. The molecular mechanisms underlying pepper thermotolerance are therefore important to understand but remain elusive. In the present study, we characterized the function of CaHSL1, encoding a HAESA-LIKE (HSL) receptor-like protein kinase (RLK), during the response of pepper to high temperature and high humidity (HTHH). CaHSL1 exhibits the typical structural features of an arginine-aspartate RLK. Transient overexpression of CaHSL1 in the mesophyll cells of Nicotiana benthamiana showed that CaHSL1 localizes throughout the cell, including the plasma membrane, cytoplasm, and the nucleus. CaHSL1 was significantly upregulated by HTHH or the exogenous application of abscisic acid but not by R. solanacearum inoculation. However, CaHSL1 was downregulated by exogenously applied salicylic acid, methyl jasmonate, or ethephon. Silencing of CaHSL1 by virus-induced gene silencing significantly was reduced tolerance to HTHH and downregulated transcript levels of an associated gene CaHSP24. In contrast, transient overexpression of CaHSL1 enhanced the transcript abundance of CaHSP24 and increased tolerance to HTHH, as manifested by enhanced optimal/maximal photochemical efficiency of photosystem II in the dark (Fv/Fm) and actual photochemical efficiency of photosystem II in the light. In addition, CaWRKY40 targeted the promoter of CaHSL1 and induced transcription during HTHH but not in response to R. solanacearum. All of these results suggest that CaHSL1 is directly modulated at the transcriptional level by CaWRKY40 and functions as a positive regulator in the response of pepper to HTHH.

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Section: Discussionsupporting

confidence: 52%

CaHSL1 Acts as a Positive Regulator of Pepper Thermotolerance Under High Humidity and Is Transcriptionally Modulated by CaWRKY40

et al. 2018

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“…The activation of bZIP17 is controlled by HS in a manner similar to the regulatory mechanism that controls the UPR. In lily ( Lilium longiflorum ), promotion of thermotolerance by LlHSFAs involves regulation of bZip factors (AtbZIP11, AtbZIP44) [ 44 ]. In addition, the response pathway of bZIPs is activated during prolonged warming [ 19 ].…”

Section: Molecular Responses Of Plants Under Hsmentioning

confidence: 99%

Plant Responses to Heat Stress: Physiology, Transcription, Noncoding RNAs, and Epigenetics

Zhao

Wang

et al. 2020

IJMS

244

126

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Global warming has increased the frequency of extreme high temperature events. High temperature is a major abiotic stress that limits the growth and production of plants. Therefore, the plant response to heat stress (HS) has been a focus of research. However, the plant response to HS involves complex physiological traits and molecular or gene networks that are not fully understood. Here, we review recent progress in the physiological (photosynthesis, cell membrane thermostability, oxidative damage, and others), transcriptional, and post-transcriptional (noncoding RNAs) regulation of the plant response to HS. We also summarize advances in understanding of the epigenetic regulation (DNA methylation, histone modification, and chromatin remodeling) and epigenetic memory underlying plant–heat interactions. Finally, we discuss the challenges and opportunities of future research in the plant response to HS.

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“…Although the function of some HSFs in thermotolerance had been well characterized, their functions in other abiotic stresses remain largely unknown (Wu et al , 2018). Many aspects need to be determined, including: Do HSFs only bind to the HSE to regulate the gene expression?…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

Zang

Wang

Zhang

et al. 2019

Journal of Experimental Botany

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Plant heat shock transcription factors (HSFs) are involved in heat and other abiotic stress responses. However, their functions in salt tolerance are little known. In this study, we characterized the function of a HSF from Arabidopsis, AtHSFA7b, in salt tolerance. AtHSFA7b is a nuclear protein with transactivation activity. ChIP-seq combined with an RNA-seq assay indicated that AtHSFA7b preferentially binds to a novel cis-acting element, termed the E-box-like motif, to regulate gene expression; it also binds to the heat shock element motif. Under salt conditions, AtHSFA7b regulates its target genes to mediate serial physiological changes, including maintaining cellular ion homeostasis, reducing water loss rate, decreasing reactive oxygen species accumulation, and adjusting osmotic potential, which ultimately leads to improved salt tolerance. Additionally, most cellulose synthase-like (CSL) and cellulose synthase (CESA) family genes were inhibited by AtHSFA7b; some of them were randomly selected for salt tolerance characterization, and they were mainly found to negatively modulate salt tolerance. By contrast, some transcription factors (TFs) were induced by AtHSFA7b; among them, we randomly identified six TFs that positively regulate salt tolerance. Thus, AtHSFA7b serves as a transactivator that positively mediates salinity tolerance mainly through binding to the E-box-like motif to regulate gene expression.

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Overexpression of lily HsfA3s in Arabidopsis confers increased thermotolerance and salt sensitivity via alterations in proline catabolism

Abstract: Overexpression of lily HsfA3s in Arabidopsis leads to changes in proline metabolism mediated via alterations in the regulation of specific bZIP transcription factors and confers increased thermotolerance and salt sensitivity.

Cited by 69 publications

References 74 publications

CaHSL1 Acts as a Positive Regulator of Pepper Thermotolerance Under High Humidity and Is Transcriptionally Modulated by CaWRKY40

CaHSL1 Acts as a Positive Regulator of Pepper Thermotolerance Under High Humidity and Is Transcriptionally Modulated by CaWRKY40

Plant Responses to Heat Stress: Physiology, Transcription, Noncoding RNAs, and Epigenetics

Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

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