Overexpression of mango alcohol dehydrogenase (MiADH1) mimics hypoxia in transgenic tomato and alters fruit flavor components

Singh, Rajesh Kumar; Srivastava, Smriti; Chidley, Hemangi G.; Nath, Paras; Sane, Vidhu A.

doi:10.1016/j.aggene.2017.10.003

Cited by 13 publications

(8 citation statements)

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“…Among the ADH and TPS gene families, the expression levels of PeADH13/26/33 and PeTPS2/3/4/24 increased gradually with fruit development, which is consistent with the increasing trend of ester and terpene metabolite contents. The same results were reported in studies of mango 66 , 67 and pear 68 . These results indicated that these genes may play an important role in passion fruit flavor biosynthesis.…”

Section: Discussionsupporting

confidence: 87%

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

et al. 2021

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Passion fruit (Passiflora edulis Sims) is an economically valuable fruit that is cultivated in tropical and subtropical regions of the world. Here, we report an ~1341.7 Mb chromosome-scale genome assembly of passion fruit, with 98.91% (~1327.18 Mb) of the assembly assigned to nine pseudochromosomes. The genome includes 23,171 protein-coding genes, and most of the assembled sequences are repetitive sequences, with long-terminal repeats (LTRs) being the most abundant. Phylogenetic analysis revealed that passion fruit diverged after Brassicaceae and before Euphorbiaceae. Ks analysis showed that two whole-genome duplication events occurred in passion fruit at 65 MYA and 12 MYA, which may have contributed to its large genome size. An integrated analysis of genomic, transcriptomic, and metabolomic data showed that ‘alpha-linolenic acid metabolism’, ‘metabolic pathways’, and ‘secondary metabolic pathways’ were the main pathways involved in the synthesis of important volatile organic compounds (VOCs) in passion fruit, and this analysis identified some candidate genes, including GDP-fucose Transporter 1-like, Tetratricopeptide repeat protein 33, protein NETWORKED 4B isoform X1, and Golgin Subfamily A member 6-like protein 22. In addition, we identified 13 important gene families in fatty acid pathways and eight important gene families in terpene pathways. Gene family analysis showed that the ACX, ADH, ALDH, and HPL gene families, especially ACX13/14/15/20, ADH13/26/33, ALDH1/4/21, and HPL4/6, were the key genes for ester synthesis, while the TPS gene family, especially PeTPS2/3/4/24, was the key gene family for terpene synthesis. This work provides insights into genome evolution and flavor trait biology and offers valuable resources for the improved cultivation of passion fruit.

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Section: Discussionsupporting

confidence: 87%

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

et al. 2021

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“…Plant metabolite production is affected by both plant development and environmental factors. Alcohols are components of plant volatiles and also act as plant stress signaling molecules [ 1 ]. Alcohol dehydrogenase (ADH, alcohol: NAD + oxidoreductase, EC 1.1.1.1) acts as a dimer that relies on NAD(P) co-factors to interconvert ethanol and acetaldehyde and other short linear alcohols/aldehyde pairs [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

The alcohol dehydrogenase gene family in sugarcane and its involvement in cold stress regulation

Ren

Wang

et al. 2020

BMC Genomics

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Background Alcohol dehydrogenases (ADHs) in plants are encoded by a multigene family. ADHs participate in growth, development, and adaptation in many plant species, but the evolution and function of the ADH gene family in sugarcane is still unclear. Results In the present study, 151 ADH genes from 17 species including 32 ADH genes in Saccharum spontaneum and 6 ADH genes in modern sugarcane cultivar R570 were identified. Phylogenetic analysis demonstrated two groups of ADH genes and suggested that these genes underwent duplication during angiosperm evolution. Whole-genome duplication (WGD)/segmental and dispersed duplications played critical roles in the expansion of ADH family in S. spontaneum and R570, respectively. ScADH3 was cloned and preferentially expressed in response to cold stress. ScADH3 conferred improved cold tolerance in E. coli cells. Ectopic expression showed that ScADH3 can also enhance cold tolerance in transgenic tobacco. The accumulation of reactive oxygen species (ROS) in leaves of transgenic tobacco was significantly lower than in wild-type tobacco. The transcript levels of ROS-related genes in transgenic tobacco increased significantly. ScADH3 seems to affect cold tolerance by regulating the ROS-related genes to maintain the ROS homeostasis. Conclusions This study depicted the size and composition of the ADH gene family in 17 species, and investigated their evolution pattern. Comparative genomics analysis among the ADH gene families of S. bicolor , R570 and S. spontaneum revealed their close evolutionary relationship. Functional analysis suggested that ScADH3 , which maintained the steady state of ROS by regulating ROS-related genes, was related to cold tolerance. These findings will facilitate research on evolutionary and functional aspects of the ADH genes in sugarcane, especially for the understanding of ScADH3 under cold stress.

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“…Alcohol dehydrogenase (ADH) catalyses the interconversion of aldehydes and their corresponding alcohols and is a key enzyme in volatile ester biosynthesis [13]. Recent works show that the overexpression of an alcohol dehydrogenase (ADH) from mango led to a change in alcohols and aldehydes related to flavour [120], with previous works also showing that overexpression of an ADH increased the level of alcohols [121]. Alcohol acyl-transferases (AAT) catalyse the transfer of an acyl-CoA to an alcohol, resulting in the synthesis of a wide range of esters [122].…”

Section: Generation Of Aromas and Flavoursmentioning

confidence: 99%

Aromas and Flavours of Fruits

Gonçalves¹,

Oliveira²,

Bacelar³

et al. 2018

Generation of Aromas and Flavours

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Aromas and flavours play an important role in horticultural crops' quality, namely in fruits. Plant breeders have made considerable advances producing cultivars with higher yields, resistant to pests and diseases, or with high nutritional quality, without paying enough attention to flavour quality. Indeed, consumers have the perception that fruit aromas and flavours have declined in the last years. Attention is given nowadays not only to flavoured compounds but also to compounds with antioxidant activity such as phenolic compounds. Fruit flavour is a combination of aroma and taste sensations. Conjugation of sugars, acids, phenolics, and hundreds of volatile compounds contribute to the fruit flavour. However, flavour and aroma depend on the variety, edaphoclimatic conditions, agronomical practices and postharvest handling. This chapter reviews the aromas and flavours of the most important fruits and discusses the most recent advances in the genomics, biochemistry and biotechnology of aromas and flavours.

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Overexpression of mango alcohol dehydrogenase (MiADH1) mimics hypoxia in transgenic tomato and alters fruit flavor components

Cited by 13 publications

References 62 publications

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

The alcohol dehydrogenase gene family in sugarcane and its involvement in cold stress regulation

Aromas and Flavours of Fruits

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