Overexpression of MdMADS5, an APETALA1-like gene of apple, causes early flowering in transgenic Arabidopsis

Kotoda, Nobuhiro; Wada, Masato; Kusaba, Shinnosuke; Kano-Murakami, Yuriko; Masuda, Takeshi; Soejima, Junichi

doi:10.1016/s0168-9452(02)00024-9

Cited by 84 publications

(50 citation statements)

References 25 publications

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“…A MADS-box gene (oligo 173646) that is related to the AP1 class of MADS-box transcription factors showed a decrease in expression. The apple gene (oligo 173646) has previously been described as MdMADS5 and shown to have AP1-like properties, promoting flowering in Arabidopsis (Kotoda et al, 2002). Also identified were two squamosa-binding proteins that change in expression upon the detection of ethylene (oligos 121375, 139438).…”

Section: The Regulation Of Aroma Biosynthetic Genes By Transcription mentioning

confidence: 99%

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

et al. 2007

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Ethylene is the major effector of ripening in many fleshy fruits. In apples (Malus x domestica) the addition of ethylene causes a climacteric burst of respiration, an increase in aroma, and softening of the flesh. We have generated a transgenic line of 'Royal Gala' apple that produces no detectable levels of ethylene using antisense ACC OXIDASE, resulting in apples with no ethyleneinduced ripening attributes. In response to external ethylene these antisense fruits undergo a normal climacteric burst and produced increasing concentrations of ester, polypropanoid, and terpene volatile compounds over an 8-d period. A total of 186 candidate genes that might be involved in the production of these compounds were mined from expressed sequence tags databases and full sequence obtained. Expression patterns of 179 of these were assessed using a 15,720 oligonucleotide apple microarray. Based on sequence similarity and gene expression patterns we identified 17 candidate genes that are likely to be ethylene control points for aroma production in apple. While many of the biosynthetic steps in these pathways were represented by gene families containing two or more genes, expression patterns revealed that only a single member is typically regulated by ethylene. Only certain points within the aroma biosynthesis pathways were regulated by ethylene. Often the first step, and in all pathways the last steps, contained enzymes that were ethylene regulated. This analysis suggests that the initial and final enzymatic steps with the biosynthetic pathways are important transcriptional regulation points for aroma production in apple.

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Section: The Regulation Of Aroma Biosynthetic Genes By Transcription mentioning

confidence: 99%

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

et al. 2007

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“…Homologues of LFY, AP1, and TFL1 have been isolated from various fruit tree species, such as grapevine (Vitis vinifera), apple (Malus × domestica), Japanese pear (Pyrus pyrifolia), European pear (P. communis), quince (Cydonia oblonga), Chinese quince (Chaenomeles sinensis), and loquat (Eriobotrya japonica) (Carmona et al, 2002;Esumi et al, 2005;Kotoda and Wada, 2005;Kotoda et al, 2002). Furthermore, MdTFL1, a homolog of TFL1, maintains the inflorescence meristem in Arabidopsis (Kotoda and Wada, 2005), and the antisense expression of MdTFL1 shortens the juvenile phase in transgenic apple trees (Kotoda et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Early Flowering Phenotype in Transgenic Pears (Pyrus communis L.) Expressing the CiFT Gene

Matsuda

Ikeda

Kurosaka

et al. 2009

J. Japan. Soc. Hort. Sci.

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We transformed the European pear (Pyrus communis L.) 'La France' and 'Ballade' with the Citrus FLOWERING LOCUS T (CiFT) gene, which induces early flowering. Subsequent DNA blot analysis of the transformed and wild-type plants indicated that the transformed plants carried 1-4 copies of CiFT. Of the 8 transformed 'La France' and 7 transgenic 'Ballade' plant lines obtained, 7 and 5 lines respectively, flowered early when cultivated in vitro in a micropropagation medium. No correlation was found between the CiFT copy number and early flowering in vitro, however, the results of RNA gel blot analysis indicated a strong correlation between the expression of the CiFT gene and floral bud differentiation in vitro. Flowers that developed in vitro differed from wild-type flowers in terms of phenotypic traits, such as the number of pistils, petals, sepals, and stamens. To investigate the inheritance pattern of the CiFT gene, we obtained 15 seeds from the fruits of P. communis L. 'Bartlett' plants that were cross-pollinated with transgenic line No. 6, which had the second highest expression of the transgene. Of 7 seedlings that expressed the CiFT gene, 5 flowered within 10 months after they were transferred to a greenhouse. This result indicated that the CiFT gene induced early flowering in the transformed pear plants and that their progeny inherited the early flowering phenotype.

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“…Recently, investigations of genes related to floral bud, floral organ, and fruit formation have been conducted in the apple. The meristem identity genes AFL1, AFL2, MdTFL1, and MdAP1 (MdMADS5) have been isolated and their expression has been investigated in relation to early flowering (Kotoda and Wada, 2005;Kotoda et al, 2000Kotoda et al, , 2002Kotoda et al, , 2006Sung et al, 1999;Wada et al, 2002). In addition, the floral organ identity genes MdMADS1 to MdMADS15, MdPI, and MdTM6 have been isolated (Kitahara et al, 2004;Sung and An, 1997Van der Linden et al, 2002;Yao et al, 1999Yao et al, , 2001).…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of MdPI, the PISTILLATA Gene Homologue of Apple, in Arabidopsis

Tanaka¹,

Wada²,

Komori³

et al. 2007

J. Japan. Soc. Hort. Sci.

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Some cultivars of apple (Malus × domestica Borkh.) show parthenocarpy. In these cultivars, the MdPISTILLATA (MdPI) gene, which is homologue of the PISTILLATA (PI) gene of Arabidopsis thaliana, does not function by the insertion of a retrotransposon into MdPI. MdPI is a class B floral organ identity gene belonging to the MADSbox gene family. When MdPI was overexpressed in Arabidopsis Columbia ecotype under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the sepals showed petaloid changes. In the case of the pi-1 mutant of Arabidopsis Landsberg erecta, apple MdPI overexpression led the pi-1 mutant to recover normal petals and carpeloid stamens. In detailed observations with a scanning electron microscope, we observed that the filaments were also normally recovered. However, the anthers were not. We isolated the MdPI promoter from the apple cultivar 'Jonathan', and the uidA gene which encodes β-glucuronidase (GUS) was fused as a reporter gene. The MdPI promoter fused with GUS was designated p1MDPI.1G. Just petals and stamens of Arabidopsis plants transformed with p1MDPI.1G had GUS activity. These results indicated that the MdPI gene was related to the development of petals and stamens, and formed petals and filaments. Therefore, the MdPI gene had a function equal to PI, although the relation between MdPI and parthenocarpy in the apple is unknown.

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Overexpression of MdMADS5, an APETALA1-like gene of apple, causes early flowering in transgenic Arabidopsis

Cited by 84 publications

References 25 publications

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

Early Flowering Phenotype in Transgenic Pears (Pyrus communis L.) Expressing the CiFT Gene

Functional Analysis of MdPI, the PISTILLATA Gene Homologue of Apple, in Arabidopsis

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